Loop-mediated isothermal amplification as an emerging technology for detection of <it>Yersinia ruckeri </it>the causative agent of enteric red mouth disease in fish
<p>Abstract</p> <p>Background</p> <p>Enteric Redmouth (ERM) disease also known as Yersiniosis is a contagious disease affecting salmonids, mainly rainbow trout. The causative agent is the gram-negative bacterium <it>Yersinia ruckeri</it>. The disease can be...
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| Format: | Article |
| Language: | English |
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BMC
2008-08-01
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| Series: | BMC Veterinary Research |
| Online Access: | http://www.biomedcentral.com/1746-6148/4/31 |
| _version_ | 1811297505677148160 |
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| author | Soliman Hatem Saleh Mona El-Matbouli Mansour |
| author_facet | Soliman Hatem Saleh Mona El-Matbouli Mansour |
| author_sort | Soliman Hatem |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Enteric Redmouth (ERM) disease also known as Yersiniosis is a contagious disease affecting salmonids, mainly rainbow trout. The causative agent is the gram-negative bacterium <it>Yersinia ruckeri</it>. The disease can be diagnosed by isolation and identification of the causative agent, or detection of the <it>Pathogen </it>using fluorescent antibody tests, ELISA and PCR assays. These diagnostic methods are laborious, time consuming and need well trained personnel.</p> <p>Results</p> <p>A loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for detection of <it>Y. ruckeri </it>the etiological agent of enteric red mouth (ERM) disease in salmonids. The assay was optimised to amplify the <it>yruI/yruR </it>gene, which encodes <it>Y. ruckeri </it>quorum sensing system, in the presence of a specific primer set and <it>Bst </it>DNA polymerase at an isothermal temperature of 63°C for one hour. Amplification products were detected by visual inspection, agarose gel electrophoresis and by real-time monitoring of turbidity resulted by formation of LAMP amplicons. Digestion with <it>Hph</it>I restriction enzyme demonstrated that the amplified product was unique. The specificity of the assay was verified by the absence of amplification products when tested against related bacteria. The assay had 10-fold higher sensitivity compared with conventional PCR and successfully detected <it>Y. ruckeri </it>not only in pure bacterial culture but also in tissue homogenates of infected fish.</p> <p>Conclusion</p> <p>The ERM-LAMP assay represents a practical alternative to the microbiological approach for rapid, sensitive and specific detection of <it>Y. ruckeri </it>in fish farms. The assay is carried out in one hour and needs only a heating block or water bath as laboratory furniture. The advantages of the ERM-LAMP assay make it a promising tool for molecular detection of enteric red mouth disease in fish farms.</p> |
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| format | Article |
| id | doaj.art-992b33fabf074a7591130efc97d34039 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-13T06:05:23Z |
| publishDate | 2008-08-01 |
| publisher | BMC |
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| series | BMC Veterinary Research |
| spelling | doaj.art-992b33fabf074a7591130efc97d340392022-12-22T02:59:16ZengBMCBMC Veterinary Research1746-61482008-08-01413110.1186/1746-6148-4-31Loop-mediated isothermal amplification as an emerging technology for detection of <it>Yersinia ruckeri </it>the causative agent of enteric red mouth disease in fishSoliman HatemSaleh MonaEl-Matbouli Mansour<p>Abstract</p> <p>Background</p> <p>Enteric Redmouth (ERM) disease also known as Yersiniosis is a contagious disease affecting salmonids, mainly rainbow trout. The causative agent is the gram-negative bacterium <it>Yersinia ruckeri</it>. The disease can be diagnosed by isolation and identification of the causative agent, or detection of the <it>Pathogen </it>using fluorescent antibody tests, ELISA and PCR assays. These diagnostic methods are laborious, time consuming and need well trained personnel.</p> <p>Results</p> <p>A loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for detection of <it>Y. ruckeri </it>the etiological agent of enteric red mouth (ERM) disease in salmonids. The assay was optimised to amplify the <it>yruI/yruR </it>gene, which encodes <it>Y. ruckeri </it>quorum sensing system, in the presence of a specific primer set and <it>Bst </it>DNA polymerase at an isothermal temperature of 63°C for one hour. Amplification products were detected by visual inspection, agarose gel electrophoresis and by real-time monitoring of turbidity resulted by formation of LAMP amplicons. Digestion with <it>Hph</it>I restriction enzyme demonstrated that the amplified product was unique. The specificity of the assay was verified by the absence of amplification products when tested against related bacteria. The assay had 10-fold higher sensitivity compared with conventional PCR and successfully detected <it>Y. ruckeri </it>not only in pure bacterial culture but also in tissue homogenates of infected fish.</p> <p>Conclusion</p> <p>The ERM-LAMP assay represents a practical alternative to the microbiological approach for rapid, sensitive and specific detection of <it>Y. ruckeri </it>in fish farms. The assay is carried out in one hour and needs only a heating block or water bath as laboratory furniture. The advantages of the ERM-LAMP assay make it a promising tool for molecular detection of enteric red mouth disease in fish farms.</p>http://www.biomedcentral.com/1746-6148/4/31 |
| spellingShingle | Soliman Hatem Saleh Mona El-Matbouli Mansour Loop-mediated isothermal amplification as an emerging technology for detection of <it>Yersinia ruckeri </it>the causative agent of enteric red mouth disease in fish BMC Veterinary Research |
| title | Loop-mediated isothermal amplification as an emerging technology for detection of <it>Yersinia ruckeri </it>the causative agent of enteric red mouth disease in fish |
| title_full | Loop-mediated isothermal amplification as an emerging technology for detection of <it>Yersinia ruckeri </it>the causative agent of enteric red mouth disease in fish |
| title_fullStr | Loop-mediated isothermal amplification as an emerging technology for detection of <it>Yersinia ruckeri </it>the causative agent of enteric red mouth disease in fish |
| title_full_unstemmed | Loop-mediated isothermal amplification as an emerging technology for detection of <it>Yersinia ruckeri </it>the causative agent of enteric red mouth disease in fish |
| title_short | Loop-mediated isothermal amplification as an emerging technology for detection of <it>Yersinia ruckeri </it>the causative agent of enteric red mouth disease in fish |
| title_sort | loop mediated isothermal amplification as an emerging technology for detection of it yersinia ruckeri it the causative agent of enteric red mouth disease in fish |
| url | http://www.biomedcentral.com/1746-6148/4/31 |
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