A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory
The function of biomolecules is tightly linked to their structure, and changes therein. Time-resolved X-ray solution scattering has proven a powerful technique for interrogating structural changes and signal transduction in photoreceptor proteins. However, these only represent a small fraction of th...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
International Union of Crystallography
2022-03-01
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| Series: | Journal of Synchrotron Radiation |
| Subjects: | |
| Online Access: | http://scripts.iucr.org/cgi-bin/paper?S1600577522000996 |
| _version_ | 1818772448947994624 |
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| author | Oskar Berntsson Ann E. Terry Tomás S. Plivelic |
| author_facet | Oskar Berntsson Ann E. Terry Tomás S. Plivelic |
| author_sort | Oskar Berntsson |
| collection | DOAJ |
| description | The function of biomolecules is tightly linked to their structure, and changes therein. Time-resolved X-ray solution scattering has proven a powerful technique for interrogating structural changes and signal transduction in photoreceptor proteins. However, these only represent a small fraction of the biological macromolecules of interest. More recently, laser-induced temperature jumps have been introduced as a more general means of initiating structural changes in biomolecules. Here we present the development of a setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline, primarily using infrared laser light to trigger a temperature increase, and structural changes. We present results that highlight the characteristics of this setup along with data showing structural changes in lysozyme caused by a temperature jump. Further developments and applications of the setup are also discussed. |
| first_indexed | 2024-12-18T10:09:31Z |
| format | Article |
| id | doaj.art-992cef0bed3849549a03d175c859f030 |
| institution | Directory Open Access Journal |
| issn | 1600-5775 |
| language | English |
| last_indexed | 2024-12-18T10:09:31Z |
| publishDate | 2022-03-01 |
| publisher | International Union of Crystallography |
| record_format | Article |
| series | Journal of Synchrotron Radiation |
| spelling | doaj.art-992cef0bed3849549a03d175c859f0302022-12-21T21:11:27ZengInternational Union of CrystallographyJournal of Synchrotron Radiation1600-57752022-03-0129255556210.1107/S1600577522000996ay5592A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV LaboratoryOskar Berntsson0Ann E. Terry1Tomás S. Plivelic2MAX IV Laboratory, Lund University, Lund, SwedenMAX IV Laboratory, Lund University, Lund, SwedenMAX IV Laboratory, Lund University, Lund, SwedenThe function of biomolecules is tightly linked to their structure, and changes therein. Time-resolved X-ray solution scattering has proven a powerful technique for interrogating structural changes and signal transduction in photoreceptor proteins. However, these only represent a small fraction of the biological macromolecules of interest. More recently, laser-induced temperature jumps have been introduced as a more general means of initiating structural changes in biomolecules. Here we present the development of a setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline, primarily using infrared laser light to trigger a temperature increase, and structural changes. We present results that highlight the characteristics of this setup along with data showing structural changes in lysozyme caused by a temperature jump. Further developments and applications of the setup are also discussed.http://scripts.iucr.org/cgi-bin/paper?S1600577522000996beamlinetime-resolved x-ray solution scatteringtime-resolved saxs |
| spellingShingle | Oskar Berntsson Ann E. Terry Tomás S. Plivelic A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory Journal of Synchrotron Radiation beamline time-resolved x-ray solution scattering time-resolved saxs |
| title | A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory |
| title_full | A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory |
| title_fullStr | A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory |
| title_full_unstemmed | A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory |
| title_short | A setup for millisecond time-resolved X-ray solution scattering experiments at the CoSAXS beamline at the MAX IV Laboratory |
| title_sort | setup for millisecond time resolved x ray solution scattering experiments at the cosaxs beamline at the max iv laboratory |
| topic | beamline time-resolved x-ray solution scattering time-resolved saxs |
| url | http://scripts.iucr.org/cgi-bin/paper?S1600577522000996 |
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