<i>Pleurochrysis carterae</i> Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells
In this study, we examined the effect of a hot-water extract of coccolithophore <i>Pleurochrysis carterae</i> on melanogenesis in B16F1 and B16F10 melanoma cells. <i>P. carterae</i> extract inhibited the α-melanocyte-stimulating hormone (α-MSH)-enhanced mela...
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| Format: | Article |
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MDPI AG
2019-10-01
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| Series: | Cosmetics |
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| Online Access: | https://www.mdpi.com/2079-9284/6/4/60 |
| _version_ | 1811219089795842048 |
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| author | Kazuomi Sato Yuji Yamaguchi Setsuko Sakaki Hiroyuki Takenaka |
| author_facet | Kazuomi Sato Yuji Yamaguchi Setsuko Sakaki Hiroyuki Takenaka |
| author_sort | Kazuomi Sato |
| collection | DOAJ |
| description | In this study, we examined the effect of a hot-water extract of coccolithophore <i>Pleurochrysis carterae</i> on melanogenesis in B16F1 and B16F10 melanoma cells. <i>P. carterae</i> extract inhibited the α-melanocyte-stimulating hormone (α-MSH)-enhanced melanin synthesis in B16F1 melanoma cells. <i>P. carterae</i> also inhibited unstimulated melanin synthesis in B16F10 melanoma cells. Western blotting showed that the <i>P. carterae</i> extract inhibited tyrosinase and microphthalmia-associated transcription factor (MITF) in a dose-dependent manner. The reporter assay also revealed a decline in the tyrosinase promoter activity in the presence of <i>P. carterae</i> extract. Furthermore, quantitative real-time RT-PCR analysis showed that <i>P. carterae</i> extract downregulated the mRNA levels of tyrosinase and MITF. Finally, our study demonstrated that the hot-water extract of <i>P. carterae</i> inhibits melanin synthesis via the down-regulation of MITF mRNA level. Our findings indicate that <i>P. carterae</i> extract could be a possible cosmetic ingredient. |
| first_indexed | 2024-04-12T07:19:34Z |
| format | Article |
| id | doaj.art-992e27a72a5147af9acc77d50e9a4e30 |
| institution | Directory Open Access Journal |
| issn | 2079-9284 |
| language | English |
| last_indexed | 2024-04-12T07:19:34Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cosmetics |
| spelling | doaj.art-992e27a72a5147af9acc77d50e9a4e302022-12-22T03:42:21ZengMDPI AGCosmetics2079-92842019-10-01646010.3390/cosmetics6040060cosmetics6040060<i>Pleurochrysis carterae</i> Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma CellsKazuomi Sato0Yuji Yamaguchi1Setsuko Sakaki2Hiroyuki Takenaka3Division for Animal Science, College of Agriculture, Tamagawa University, 6-1-1 Tamagawa-gakuen, Machida, Tokyo 194-8610, JapanMAC Gifu Research Institute, MicroAlgae Corporation 4-15 Akebono, Gifu 500-8148, JapanMAC Gifu Research Institute, MicroAlgae Corporation 4-15 Akebono, Gifu 500-8148, JapanMAC Gifu Research Institute, MicroAlgae Corporation 4-15 Akebono, Gifu 500-8148, JapanIn this study, we examined the effect of a hot-water extract of coccolithophore <i>Pleurochrysis carterae</i> on melanogenesis in B16F1 and B16F10 melanoma cells. <i>P. carterae</i> extract inhibited the α-melanocyte-stimulating hormone (α-MSH)-enhanced melanin synthesis in B16F1 melanoma cells. <i>P. carterae</i> also inhibited unstimulated melanin synthesis in B16F10 melanoma cells. Western blotting showed that the <i>P. carterae</i> extract inhibited tyrosinase and microphthalmia-associated transcription factor (MITF) in a dose-dependent manner. The reporter assay also revealed a decline in the tyrosinase promoter activity in the presence of <i>P. carterae</i> extract. Furthermore, quantitative real-time RT-PCR analysis showed that <i>P. carterae</i> extract downregulated the mRNA levels of tyrosinase and MITF. Finally, our study demonstrated that the hot-water extract of <i>P. carterae</i> inhibits melanin synthesis via the down-regulation of MITF mRNA level. Our findings indicate that <i>P. carterae</i> extract could be a possible cosmetic ingredient.https://www.mdpi.com/2079-9284/6/4/60coccolithophore<i>pleurochrysis carterae</i>microalgaemelanogenesistyrosinasemitf |
| spellingShingle | Kazuomi Sato Yuji Yamaguchi Setsuko Sakaki Hiroyuki Takenaka <i>Pleurochrysis carterae</i> Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells Cosmetics coccolithophore <i>pleurochrysis carterae</i> microalgae melanogenesis tyrosinase mitf |
| title | <i>Pleurochrysis carterae</i> Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells |
| title_full | <i>Pleurochrysis carterae</i> Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells |
| title_fullStr | <i>Pleurochrysis carterae</i> Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells |
| title_full_unstemmed | <i>Pleurochrysis carterae</i> Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells |
| title_short | <i>Pleurochrysis carterae</i> Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells |
| title_sort | i pleurochrysis carterae i hot water extract inhibits melanogenesis in murine melanoma cells |
| topic | coccolithophore <i>pleurochrysis carterae</i> microalgae melanogenesis tyrosinase mitf |
| url | https://www.mdpi.com/2079-9284/6/4/60 |
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