Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin

Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin

Abstract Indirubin is a bisindole compound for the treatment of chronic myelocytic leukemia. Here, we presented a structure-guided method to improve the activity of a flavin-containing monooxygenase (bFMO) for the efficient production of indirubin in Escherichia coli. A flexible loop interlocked wit...

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Bibliographic Details
Main Authors: Bing-Yao Sun, Hua-Lu Sui, Zi-Wei Liu, Xin-Yi Tao, Bei Gao, Ming Zhao, Yu-Shu Ma, Jian Zhao, Min Liu, Feng-Qing Wang, Dong-Zhi Wei
Format: Article
Language:English
Published: SpringerOpen 2022-06-01
Series:Bioresources and Bioprocessing
Subjects:
Flavin-containing monooxygenase
Indirubin
Loop region
Substrate tunnel
Structure-guided enzyme engineering
Microbial synthesis
Online Access:https://doi.org/10.1186/s40643-022-00559-7

Internet

https://doi.org/10.1186/s40643-022-00559-7

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