Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis
Abstract RNF20, an E3 ligase critical for monoubiquitination of histone H2B at lysine 120 (H2Bub), has been implicated in the regulation of various cellar processes; however, its physiological roles in adipocytes remain poorly characterized. Here, we report that the adipocyte-specific knockout of Rn...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2020-08-01
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| Series: | Protein & Cell |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s13238-020-00770-2 |
| _version_ | 1797698976925876224 |
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| author | Xiaojuan Liang Cong Tao Jianfei Pan Lilan Zhang Lulu Liu Ying Zhao Yiping Fan Chunwei Cao Jiali Liu Jin Zhang Sin Man Lam Guanghou Shui Wanzhu Jin Wei Li Jianguo Zhao Kui Li Yanfang Wang |
| author_facet | Xiaojuan Liang Cong Tao Jianfei Pan Lilan Zhang Lulu Liu Ying Zhao Yiping Fan Chunwei Cao Jiali Liu Jin Zhang Sin Man Lam Guanghou Shui Wanzhu Jin Wei Li Jianguo Zhao Kui Li Yanfang Wang |
| author_sort | Xiaojuan Liang |
| collection | DOAJ |
| description | Abstract RNF20, an E3 ligase critical for monoubiquitination of histone H2B at lysine 120 (H2Bub), has been implicated in the regulation of various cellar processes; however, its physiological roles in adipocytes remain poorly characterized. Here, we report that the adipocyte-specific knockout of Rnf20 (ASKO) in mice led to progressive fat loss, organomegaly and hyperinsulinemia. Despite signs of hyperinsulinemia, normal insulin sensitivity and improved glucose tolerance were observed in the young and aged CD-fed ASKO mice. In addition, high-fat diet-fed ASKO mice developed severe liver steatosis. Moreover, we observed that the ASKO mice were extremely sensitive to a cold environment due to decreased expression levels of brown adipose tissue (BAT) selective genes, including uncoupling protein 1 (Ucp1), and impaired mitochondrial functions. Significantly decreased levels of peroxisome proliferator-activated receptor gamma (Pparγ) were observed in the gonadal white adipose tissues (gWAT) from the ASKO mice, suggesting that Rnf20 regulates adipogenesis, at least in part, through Pparγ. Rosiglitazone-treated ASKO mice exhibited increased fat mass compared to that of the non-treated ASKO mice. Collectively, our results illustrate the critical role of RNF20 in control of white and brown adipose tissue development and physiological function. |
| first_indexed | 2024-03-12T04:01:53Z |
| format | Article |
| id | doaj.art-99303c0053aa495e952c31c70b879b6f |
| institution | Directory Open Access Journal |
| issn | 1674-800X 1674-8018 |
| language | English |
| last_indexed | 2024-03-12T04:01:53Z |
| publishDate | 2020-08-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Protein & Cell |
| spelling | doaj.art-99303c0053aa495e952c31c70b879b6f2023-09-03T11:33:15ZengOxford University PressProtein & Cell1674-800X1674-80182020-08-0112647549210.1007/s13238-020-00770-2Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesisXiaojuan Liang0Cong Tao1Jianfei Pan2Lilan Zhang3Lulu Liu4Ying Zhao5Yiping Fan6Chunwei Cao7Jiali Liu8Jin Zhang9Sin Man Lam10Guanghou Shui11Wanzhu Jin12Wei Li13Jianguo Zhao14Kui Li15Yanfang Wang16State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural SciencesInstitute of Zoology, Chinese Academy of SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural SciencesCollege of Biological, Chemical Sciences and Engineering, Jiaxing UniversityInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesInstitute of Zoology, Chinese Academy of SciencesInstitute of Zoology, Chinese Academy of SciencesInstitute of Zoology, Chinese Academy of SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural SciencesAbstract RNF20, an E3 ligase critical for monoubiquitination of histone H2B at lysine 120 (H2Bub), has been implicated in the regulation of various cellar processes; however, its physiological roles in adipocytes remain poorly characterized. Here, we report that the adipocyte-specific knockout of Rnf20 (ASKO) in mice led to progressive fat loss, organomegaly and hyperinsulinemia. Despite signs of hyperinsulinemia, normal insulin sensitivity and improved glucose tolerance were observed in the young and aged CD-fed ASKO mice. In addition, high-fat diet-fed ASKO mice developed severe liver steatosis. Moreover, we observed that the ASKO mice were extremely sensitive to a cold environment due to decreased expression levels of brown adipose tissue (BAT) selective genes, including uncoupling protein 1 (Ucp1), and impaired mitochondrial functions. Significantly decreased levels of peroxisome proliferator-activated receptor gamma (Pparγ) were observed in the gonadal white adipose tissues (gWAT) from the ASKO mice, suggesting that Rnf20 regulates adipogenesis, at least in part, through Pparγ. Rosiglitazone-treated ASKO mice exhibited increased fat mass compared to that of the non-treated ASKO mice. Collectively, our results illustrate the critical role of RNF20 in control of white and brown adipose tissue development and physiological function.https://doi.org/10.1007/s13238-020-00770-2RNF20fat lossadipose tissue developmentthermogenesis |
| spellingShingle | Xiaojuan Liang Cong Tao Jianfei Pan Lilan Zhang Lulu Liu Ying Zhao Yiping Fan Chunwei Cao Jiali Liu Jin Zhang Sin Man Lam Guanghou Shui Wanzhu Jin Wei Li Jianguo Zhao Kui Li Yanfang Wang Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis Protein & Cell RNF20 fat loss adipose tissue development thermogenesis |
| title | Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis |
| title_full | Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis |
| title_fullStr | Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis |
| title_full_unstemmed | Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis |
| title_short | Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis |
| title_sort | rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis |
| topic | RNF20 fat loss adipose tissue development thermogenesis |
| url | https://doi.org/10.1007/s13238-020-00770-2 |
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