Hydrogen Peroxide Promotes Tomato Leaf Senescence by Regulating Antioxidant System and Hydrogen Sulfide Metabolism
Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is relatively stable among ROS (reactive oxygen species) and could act as a signal in plant cells. In the present work, detached tomato leaves were treated with exogenous H<sub>2</sub>O<sub>2</sub> at 10 m...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/13/4/475 |
| _version_ | 1827342892058804224 |
|---|---|
| author | Yue Yu Siyue Wang Wentong Guo Meihui Geng Ying Sun Wanjie Li Gaifang Yao Danfeng Zhang Hua Zhang Kangdi Hu |
| author_facet | Yue Yu Siyue Wang Wentong Guo Meihui Geng Ying Sun Wanjie Li Gaifang Yao Danfeng Zhang Hua Zhang Kangdi Hu |
| author_sort | Yue Yu |
| collection | DOAJ |
| description | Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is relatively stable among ROS (reactive oxygen species) and could act as a signal in plant cells. In the present work, detached tomato leaves were treated with exogenous H<sub>2</sub>O<sub>2</sub> at 10 mmol/L for 8 h to study the mechanism of how H<sub>2</sub>O<sub>2</sub> regulates leaf senescence. The data indicated that H<sub>2</sub>O<sub>2</sub> treatment significantly accelerated the degradation of chlorophyll and led to the upregulation of the expression of leaf senescence-related genes (<i>NYC1</i>, <i>PAO</i>, <i>PPH</i>, <i>SGR1</i>, <i>SAG12</i> and <i>SAG15</i>) during leaf senescence. H<sub>2</sub>O<sub>2</sub> treatment also induced the accumulation of H<sub>2</sub>O<sub>2</sub> and malondialdehyde (MDA), decreased POD and SOD enzyme activities and inhibited H<sub>2</sub>S production by reducing the expression of <i>LCD1/2</i> and <i>DCD1/2.</i> A correlation analysis indicated that H<sub>2</sub>O<sub>2</sub> was significantly and negatively correlated with chlorophyll, the expression of leaf senescence−related genes, and <i>LCD1/2</i> and <i>DCD1/2</i>. The principal component analysis (PCA) results show that H<sub>2</sub>S showed the highest load value followed by O<sub>2</sub><sup>•−</sup>, H<sub>2</sub>O<sub>2</sub>, <i>DCD1</i>, <i>SAG15</i>, etc. Therefore, these findings provide a basis for studying the role of H<sub>2</sub>O<sub>2</sub> in regulating detached tomato leaf senescence and demonstrated that H<sub>2</sub>O<sub>2</sub> plays a positive role in the senescence of detached leaves by repressing antioxidant enzymes and H<sub>2</sub>S production. |
| first_indexed | 2024-03-07T22:17:11Z |
| format | Article |
| id | doaj.art-9931d3cc9e594a7987895bab9d37b284 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-07T22:17:11Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-9931d3cc9e594a7987895bab9d37b2842024-02-23T15:31:50ZengMDPI AGPlants2223-77472024-02-0113447510.3390/plants13040475Hydrogen Peroxide Promotes Tomato Leaf Senescence by Regulating Antioxidant System and Hydrogen Sulfide MetabolismYue Yu0Siyue Wang1Wentong Guo2Meihui Geng3Ying Sun4Wanjie Li5Gaifang Yao6Danfeng Zhang7Hua Zhang8Kangdi Hu9School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaKey Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, ChinaHydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is relatively stable among ROS (reactive oxygen species) and could act as a signal in plant cells. In the present work, detached tomato leaves were treated with exogenous H<sub>2</sub>O<sub>2</sub> at 10 mmol/L for 8 h to study the mechanism of how H<sub>2</sub>O<sub>2</sub> regulates leaf senescence. The data indicated that H<sub>2</sub>O<sub>2</sub> treatment significantly accelerated the degradation of chlorophyll and led to the upregulation of the expression of leaf senescence-related genes (<i>NYC1</i>, <i>PAO</i>, <i>PPH</i>, <i>SGR1</i>, <i>SAG12</i> and <i>SAG15</i>) during leaf senescence. H<sub>2</sub>O<sub>2</sub> treatment also induced the accumulation of H<sub>2</sub>O<sub>2</sub> and malondialdehyde (MDA), decreased POD and SOD enzyme activities and inhibited H<sub>2</sub>S production by reducing the expression of <i>LCD1/2</i> and <i>DCD1/2.</i> A correlation analysis indicated that H<sub>2</sub>O<sub>2</sub> was significantly and negatively correlated with chlorophyll, the expression of leaf senescence−related genes, and <i>LCD1/2</i> and <i>DCD1/2</i>. The principal component analysis (PCA) results show that H<sub>2</sub>S showed the highest load value followed by O<sub>2</sub><sup>•−</sup>, H<sub>2</sub>O<sub>2</sub>, <i>DCD1</i>, <i>SAG15</i>, etc. Therefore, these findings provide a basis for studying the role of H<sub>2</sub>O<sub>2</sub> in regulating detached tomato leaf senescence and demonstrated that H<sub>2</sub>O<sub>2</sub> plays a positive role in the senescence of detached leaves by repressing antioxidant enzymes and H<sub>2</sub>S production.https://www.mdpi.com/2223-7747/13/4/475tomatohydrogen peroxideleaf senescenceantioxidant systemhydrogen sulfide |
| spellingShingle | Yue Yu Siyue Wang Wentong Guo Meihui Geng Ying Sun Wanjie Li Gaifang Yao Danfeng Zhang Hua Zhang Kangdi Hu Hydrogen Peroxide Promotes Tomato Leaf Senescence by Regulating Antioxidant System and Hydrogen Sulfide Metabolism Plants tomato hydrogen peroxide leaf senescence antioxidant system hydrogen sulfide |
| title | Hydrogen Peroxide Promotes Tomato Leaf Senescence by Regulating Antioxidant System and Hydrogen Sulfide Metabolism |
| title_full | Hydrogen Peroxide Promotes Tomato Leaf Senescence by Regulating Antioxidant System and Hydrogen Sulfide Metabolism |
| title_fullStr | Hydrogen Peroxide Promotes Tomato Leaf Senescence by Regulating Antioxidant System and Hydrogen Sulfide Metabolism |
| title_full_unstemmed | Hydrogen Peroxide Promotes Tomato Leaf Senescence by Regulating Antioxidant System and Hydrogen Sulfide Metabolism |
| title_short | Hydrogen Peroxide Promotes Tomato Leaf Senescence by Regulating Antioxidant System and Hydrogen Sulfide Metabolism |
| title_sort | hydrogen peroxide promotes tomato leaf senescence by regulating antioxidant system and hydrogen sulfide metabolism |
| topic | tomato hydrogen peroxide leaf senescence antioxidant system hydrogen sulfide |
| url | https://www.mdpi.com/2223-7747/13/4/475 |
| work_keys_str_mv | AT yueyu hydrogenperoxidepromotestomatoleafsenescencebyregulatingantioxidantsystemandhydrogensulfidemetabolism AT siyuewang hydrogenperoxidepromotestomatoleafsenescencebyregulatingantioxidantsystemandhydrogensulfidemetabolism AT wentongguo hydrogenperoxidepromotestomatoleafsenescencebyregulatingantioxidantsystemandhydrogensulfidemetabolism AT meihuigeng hydrogenperoxidepromotestomatoleafsenescencebyregulatingantioxidantsystemandhydrogensulfidemetabolism AT yingsun hydrogenperoxidepromotestomatoleafsenescencebyregulatingantioxidantsystemandhydrogensulfidemetabolism AT wanjieli hydrogenperoxidepromotestomatoleafsenescencebyregulatingantioxidantsystemandhydrogensulfidemetabolism AT gaifangyao hydrogenperoxidepromotestomatoleafsenescencebyregulatingantioxidantsystemandhydrogensulfidemetabolism AT danfengzhang hydrogenperoxidepromotestomatoleafsenescencebyregulatingantioxidantsystemandhydrogensulfidemetabolism AT huazhang hydrogenperoxidepromotestomatoleafsenescencebyregulatingantioxidantsystemandhydrogensulfidemetabolism AT kangdihu hydrogenperoxidepromotestomatoleafsenescencebyregulatingantioxidantsystemandhydrogensulfidemetabolism |