Biofilm Formation of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> in a Simulated Chicken Processing Environment
This study aims to investigate the mono- and dual-species biofilm formation of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> incubated in different culture mediums, inoculum ratios, and incubation time. The planktonic cell population and motility were examin...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/11/13/1917 |
| _version_ | 1797480008686501888 |
|---|---|
| author | Qingli Dong Linjun Sun Taisong Fang Yuan Wang Zhuosi Li Xiang Wang Mengjie Wu Hongzhi Zhang |
| author_facet | Qingli Dong Linjun Sun Taisong Fang Yuan Wang Zhuosi Li Xiang Wang Mengjie Wu Hongzhi Zhang |
| author_sort | Qingli Dong |
| collection | DOAJ |
| description | This study aims to investigate the mono- and dual-species biofilm formation of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> incubated in different culture mediums, inoculum ratios, and incubation time. The planktonic cell population and motility were examined to understand the correlation with biofilm formation. The results showed that chicken juice significantly inhibited the biofilm formation of <i>L. monocytogenes</i> (<i>p</i> < 0.05). <i>Pseudomonas aeruginosa</i> was the dominant bacteria in the dual-species biofilm formation in the trypticase soy broth medium. The dynamic changes in biofilm formation were not consistent with the different culture conditions. The growth of planktonic <i>L. monocytogenes</i> and <i>P. aeruginosa</i> in the suspension was inconsistent with their growth in the biofilms. There was no significant correlation between motility and biofilm formation of <i>L. monocytogenes</i> and <i>P. aeruginosa</i>. Moreover, scanning electron microscopy (SEM) results revealed that the biofilm structure of <i>L. monocytogenes</i> was loose. At the same time, <i>P. aeruginosa</i> formed a relatively dense network in mono-species biofilms in an initial adhesion stage (24 h). SEM results also showed that <i>P. aeruginosa</i> was dominant in the dual-species biofilms. Overall, these results could provide a theoretical reference for preventing and controlling the biofilm formation of <i>L. monocytogenes</i> and <i>P. aeruginosa</i> in the food processing environment in the future. |
| first_indexed | 2024-03-09T21:53:57Z |
| format | Article |
| id | doaj.art-99348444ac224978b66b99e52e3d1830 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T21:53:57Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-99348444ac224978b66b99e52e3d18302023-11-23T20:01:24ZengMDPI AGFoods2304-81582022-06-011113191710.3390/foods11131917Biofilm Formation of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> in a Simulated Chicken Processing EnvironmentQingli Dong0Linjun Sun1Taisong Fang2Yuan Wang3Zhuosi Li4Xiang Wang5Mengjie Wu6Hongzhi Zhang7School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaSchool of Food and Drug, Shanghai Zhongqiao Vocational and Technical University, Shanghai 201514, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, ChinaThis study aims to investigate the mono- and dual-species biofilm formation of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> incubated in different culture mediums, inoculum ratios, and incubation time. The planktonic cell population and motility were examined to understand the correlation with biofilm formation. The results showed that chicken juice significantly inhibited the biofilm formation of <i>L. monocytogenes</i> (<i>p</i> < 0.05). <i>Pseudomonas aeruginosa</i> was the dominant bacteria in the dual-species biofilm formation in the trypticase soy broth medium. The dynamic changes in biofilm formation were not consistent with the different culture conditions. The growth of planktonic <i>L. monocytogenes</i> and <i>P. aeruginosa</i> in the suspension was inconsistent with their growth in the biofilms. There was no significant correlation between motility and biofilm formation of <i>L. monocytogenes</i> and <i>P. aeruginosa</i>. Moreover, scanning electron microscopy (SEM) results revealed that the biofilm structure of <i>L. monocytogenes</i> was loose. At the same time, <i>P. aeruginosa</i> formed a relatively dense network in mono-species biofilms in an initial adhesion stage (24 h). SEM results also showed that <i>P. aeruginosa</i> was dominant in the dual-species biofilms. Overall, these results could provide a theoretical reference for preventing and controlling the biofilm formation of <i>L. monocytogenes</i> and <i>P. aeruginosa</i> in the food processing environment in the future.https://www.mdpi.com/2304-8158/11/13/1917<i>Listeria monocytogenes</i><i>Pseudomonas aeruginosa</i>biofilm formationchicken processing environment |
| spellingShingle | Qingli Dong Linjun Sun Taisong Fang Yuan Wang Zhuosi Li Xiang Wang Mengjie Wu Hongzhi Zhang Biofilm Formation of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> in a Simulated Chicken Processing Environment Foods <i>Listeria monocytogenes</i> <i>Pseudomonas aeruginosa</i> biofilm formation chicken processing environment |
| title | Biofilm Formation of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> in a Simulated Chicken Processing Environment |
| title_full | Biofilm Formation of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> in a Simulated Chicken Processing Environment |
| title_fullStr | Biofilm Formation of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> in a Simulated Chicken Processing Environment |
| title_full_unstemmed | Biofilm Formation of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> in a Simulated Chicken Processing Environment |
| title_short | Biofilm Formation of <i>Listeria monocytogenes</i> and <i>Pseudomonas aeruginosa</i> in a Simulated Chicken Processing Environment |
| title_sort | biofilm formation of i listeria monocytogenes i and i pseudomonas aeruginosa i in a simulated chicken processing environment |
| topic | <i>Listeria monocytogenes</i> <i>Pseudomonas aeruginosa</i> biofilm formation chicken processing environment |
| url | https://www.mdpi.com/2304-8158/11/13/1917 |
| work_keys_str_mv | AT qinglidong biofilmformationofilisteriamonocytogenesiandipseudomonasaeruginosaiinasimulatedchickenprocessingenvironment AT linjunsun biofilmformationofilisteriamonocytogenesiandipseudomonasaeruginosaiinasimulatedchickenprocessingenvironment AT taisongfang biofilmformationofilisteriamonocytogenesiandipseudomonasaeruginosaiinasimulatedchickenprocessingenvironment AT yuanwang biofilmformationofilisteriamonocytogenesiandipseudomonasaeruginosaiinasimulatedchickenprocessingenvironment AT zhuosili biofilmformationofilisteriamonocytogenesiandipseudomonasaeruginosaiinasimulatedchickenprocessingenvironment AT xiangwang biofilmformationofilisteriamonocytogenesiandipseudomonasaeruginosaiinasimulatedchickenprocessingenvironment AT mengjiewu biofilmformationofilisteriamonocytogenesiandipseudomonasaeruginosaiinasimulatedchickenprocessingenvironment AT hongzhizhang biofilmformationofilisteriamonocytogenesiandipseudomonasaeruginosaiinasimulatedchickenprocessingenvironment |