Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance
This paper proposes a simple approach to optimize the operating frequency band of a lab-on-a-chip based on bio-impedance cytometry for a single cell. It mainly concerns applications in low-conductivity media. Bio-impedance allows for the characterization of low cell concentration or single cells by...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/19/15/3366 |
| _version_ | 1811187592126791680 |
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| author | Julien Claudel Arthur Luiz Alves De Araujo Mustapha Nadi Djilali Kourtiche |
| author_facet | Julien Claudel Arthur Luiz Alves De Araujo Mustapha Nadi Djilali Kourtiche |
| author_sort | Julien Claudel |
| collection | DOAJ |
| description | This paper proposes a simple approach to optimize the operating frequency band of a lab-on-a-chip based on bio-impedance cytometry for a single cell. It mainly concerns applications in low-conductivity media. Bio-impedance allows for the characterization of low cell concentration or single cells by providing an electrical signature. Thus, it may be necessary to perform impedance measurements up to several tens of megahertz in order to extract the internal cell signature. In the case of single cells, characterization is performed in a very small volume down to 1 pL. At the same time, measured impedances increase from tens of kilo-ohms for physiological liquids up to several mega-ohms for low conductivity media. This is, for example, the case for water analysis. At frequencies above hundreds of kilohertz, parasitic effects, such as coupling capacitances, can prevail over the impedance of the sample and completely short-circuit measurements. To optimize the sensor under these conditions, a complete model of a cytometry device was developed, including parasitic coupling capacitances of the sensor to take into account all the impedances. It appears that it is possible to increase the pass band by optimizing track geometries and placement without changing the sensing area. This assumption was obtained by measuring and comparing electrical properties of yeast cells in a low-conductivity medium (tap water). Decreased coupling capacitance by a factor higher than 10 was obtained compared with a previous non-optimized sensor, which allowed for the impedance measurement of all electrical properties of cells as small as yeast cells in a low-conductivity medium. |
| first_indexed | 2024-04-11T14:06:05Z |
| format | Article |
| id | doaj.art-9939730419844662bb0a756f1bb30c51 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T14:06:05Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-9939730419844662bb0a756f1bb30c512022-12-22T04:19:54ZengMDPI AGSensors1424-82202019-07-011915336610.3390/s19153366s19153366Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-ImpedanceJulien Claudel0Arthur Luiz Alves De Araujo1Mustapha Nadi2Djilali Kourtiche3Institut Jean Lamour Lorraine University, French National Center for Scientific Research (CNRS–Unité Mixte de Recherche 7198), 54011 Nancy, FranceInstitut Jean Lamour Lorraine University, French National Center for Scientific Research (CNRS–Unité Mixte de Recherche 7198), 54011 Nancy, FranceInstitut Jean Lamour Lorraine University, French National Center for Scientific Research (CNRS–Unité Mixte de Recherche 7198), 54011 Nancy, FranceInstitut Jean Lamour Lorraine University, French National Center for Scientific Research (CNRS–Unité Mixte de Recherche 7198), 54011 Nancy, FranceThis paper proposes a simple approach to optimize the operating frequency band of a lab-on-a-chip based on bio-impedance cytometry for a single cell. It mainly concerns applications in low-conductivity media. Bio-impedance allows for the characterization of low cell concentration or single cells by providing an electrical signature. Thus, it may be necessary to perform impedance measurements up to several tens of megahertz in order to extract the internal cell signature. In the case of single cells, characterization is performed in a very small volume down to 1 pL. At the same time, measured impedances increase from tens of kilo-ohms for physiological liquids up to several mega-ohms for low conductivity media. This is, for example, the case for water analysis. At frequencies above hundreds of kilohertz, parasitic effects, such as coupling capacitances, can prevail over the impedance of the sample and completely short-circuit measurements. To optimize the sensor under these conditions, a complete model of a cytometry device was developed, including parasitic coupling capacitances of the sensor to take into account all the impedances. It appears that it is possible to increase the pass band by optimizing track geometries and placement without changing the sensing area. This assumption was obtained by measuring and comparing electrical properties of yeast cells in a low-conductivity medium (tap water). Decreased coupling capacitance by a factor higher than 10 was obtained compared with a previous non-optimized sensor, which allowed for the impedance measurement of all electrical properties of cells as small as yeast cells in a low-conductivity medium.https://www.mdpi.com/1424-8220/19/15/3366biosensorimpedance spectroscopymicrofluidicsingle cellcytometry |
| spellingShingle | Julien Claudel Arthur Luiz Alves De Araujo Mustapha Nadi Djilali Kourtiche Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance Sensors biosensor impedance spectroscopy microfluidic single cell cytometry |
| title | Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance |
| title_full | Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance |
| title_fullStr | Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance |
| title_full_unstemmed | Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance |
| title_short | Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance |
| title_sort | lab on a chip device for yeast cell characterization in low conductivity media combining cytometry and bio impedance |
| topic | biosensor impedance spectroscopy microfluidic single cell cytometry |
| url | https://www.mdpi.com/1424-8220/19/15/3366 |
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