Prognostic significance of RBP2-H1 variant of JARID1B in melanoma
Abstract Background Histone demethylase JARID1B plays several context dependent roles in epigenetic regulation of cellular differentiation in normal development and is highly expressed in multiple human cancers. The protein is a strong transcriptional repressor capable of downregulating numerous gen...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | BMC Cancer |
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| Online Access: | http://link.springer.com/article/10.1186/s12885-017-3836-x |
| _version_ | 1818932898982526976 |
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| author | Łukasz Kuźbicki Dariusz Lange Agata Stanek-Widera Barbara W. Chwirot |
| author_facet | Łukasz Kuźbicki Dariusz Lange Agata Stanek-Widera Barbara W. Chwirot |
| author_sort | Łukasz Kuźbicki |
| collection | DOAJ |
| description | Abstract Background Histone demethylase JARID1B plays several context dependent roles in epigenetic regulation of cellular differentiation in normal development and is highly expressed in multiple human cancers. The protein is a strong transcriptional repressor capable of downregulating numerous genes. There are three splicing isoforms of JARID1B, however the links between the protein structure and function are not clear. The expression pattern of JARID1B in human melanoma seems to be different from observed in other cancers. Moreover, up to now no data on the impact of JARID1B expression in cutaneous melanoma on the patients’ prognosis have been reported. Methods We investigated immunohistochemically the association of intratumoral expression of total JARID1B protein and its RBP2-H1 isoform in primary and metastatic melanomas with prognosis for the patients. Results Expression of both total JARID1B protein and its RBP2-H1 variant was found in all the melanomas investigated. Our results indicate, however, that only high (above 90% of the cells) intratumoral expression of RBP2-H1 can be considered prognostic factor associated with worse overall survival of the patients. Conclusions Such results if considered together with data demonstrating a switch to enhanced expression of RBP2-H1 at early stages of malignant transformation of melanocytes are in agreement with hypothetical crucial role of JARID1B in the course of melanoma development and progression and suggest that altered splicing of JARID1B may be important factor increasing melanoma aggressiveness. |
| first_indexed | 2024-12-20T04:39:49Z |
| format | Article |
| id | doaj.art-9939d6d0207b4118b04cbf41d4040e60 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-20T04:39:49Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-9939d6d0207b4118b04cbf41d4040e602022-12-21T19:53:09ZengBMCBMC Cancer1471-24072017-12-011711710.1186/s12885-017-3836-xPrognostic significance of RBP2-H1 variant of JARID1B in melanomaŁukasz Kuźbicki0Dariusz Lange1Agata Stanek-Widera2Barbara W. Chwirot3Department of Medical Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus UniversityDepartment of Tumor Pathology, Oncology Center – Maria Skłodowska-Curie InstituteDepartment of Tumor Pathology, Oncology Center – Maria Skłodowska-Curie InstituteDepartment of Medical Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus UniversityAbstract Background Histone demethylase JARID1B plays several context dependent roles in epigenetic regulation of cellular differentiation in normal development and is highly expressed in multiple human cancers. The protein is a strong transcriptional repressor capable of downregulating numerous genes. There are three splicing isoforms of JARID1B, however the links between the protein structure and function are not clear. The expression pattern of JARID1B in human melanoma seems to be different from observed in other cancers. Moreover, up to now no data on the impact of JARID1B expression in cutaneous melanoma on the patients’ prognosis have been reported. Methods We investigated immunohistochemically the association of intratumoral expression of total JARID1B protein and its RBP2-H1 isoform in primary and metastatic melanomas with prognosis for the patients. Results Expression of both total JARID1B protein and its RBP2-H1 variant was found in all the melanomas investigated. Our results indicate, however, that only high (above 90% of the cells) intratumoral expression of RBP2-H1 can be considered prognostic factor associated with worse overall survival of the patients. Conclusions Such results if considered together with data demonstrating a switch to enhanced expression of RBP2-H1 at early stages of malignant transformation of melanocytes are in agreement with hypothetical crucial role of JARID1B in the course of melanoma development and progression and suggest that altered splicing of JARID1B may be important factor increasing melanoma aggressiveness.http://link.springer.com/article/10.1186/s12885-017-3836-xJARID1BRBP2-H1 variant of JARID1BMelanomaPatient’s survivalImmunohistochemistry |
| spellingShingle | Łukasz Kuźbicki Dariusz Lange Agata Stanek-Widera Barbara W. Chwirot Prognostic significance of RBP2-H1 variant of JARID1B in melanoma BMC Cancer JARID1B RBP2-H1 variant of JARID1B Melanoma Patient’s survival Immunohistochemistry |
| title | Prognostic significance of RBP2-H1 variant of JARID1B in melanoma |
| title_full | Prognostic significance of RBP2-H1 variant of JARID1B in melanoma |
| title_fullStr | Prognostic significance of RBP2-H1 variant of JARID1B in melanoma |
| title_full_unstemmed | Prognostic significance of RBP2-H1 variant of JARID1B in melanoma |
| title_short | Prognostic significance of RBP2-H1 variant of JARID1B in melanoma |
| title_sort | prognostic significance of rbp2 h1 variant of jarid1b in melanoma |
| topic | JARID1B RBP2-H1 variant of JARID1B Melanoma Patient’s survival Immunohistochemistry |
| url | http://link.springer.com/article/10.1186/s12885-017-3836-x |
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