<it>In vivo </it>imaging of pancreatic tumours and liver metastases using 7 Tesla MRI in a murine orthotopic pancreatic cancer model and a liver metastases model
<p>Abstract</p> <p>Background</p> <p>Pancreatic cancer is the fourth leading cause of tumour death in the western world. However, appropriate tumour models are scarce. Here we present a syngeneic murine pancreatic cancer model using 7 Tesla MRI and evaluate its clinical...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-01-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/11/40 |
| _version_ | 1818758633827074048 |
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| author | Hadlich Stefan Lerch Markus M Mayerle Julia Weiss Ulrich F Kuehn Sandra Diedrich Stephan Seubert Florian Roese Sebastian Speerforck Sven Kühn Jens-P Albers Nele Sendler Matthias Kaeding André Partecke Ivo L Hosten Norbert Heidecke Claus-D Puls Ralf von Bernstorff Wolfram |
| author_facet | Hadlich Stefan Lerch Markus M Mayerle Julia Weiss Ulrich F Kuehn Sandra Diedrich Stephan Seubert Florian Roese Sebastian Speerforck Sven Kühn Jens-P Albers Nele Sendler Matthias Kaeding André Partecke Ivo L Hosten Norbert Heidecke Claus-D Puls Ralf von Bernstorff Wolfram |
| author_sort | Hadlich Stefan |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Pancreatic cancer is the fourth leading cause of tumour death in the western world. However, appropriate tumour models are scarce. Here we present a syngeneic murine pancreatic cancer model using 7 Tesla MRI and evaluate its clinical relevance and applicability.</p> <p>Methods</p> <p>6606PDA murine pancreatic cancer cells were orthotopically injected into the pancreatic head. Liver metastases were induced through splenic injection. Animals were analyzed by MRI three and five weeks following injection. Tumours were detected using T2-weighted high resolution sequences. Tumour volumes were determined by callipers and MRI. Liver metastases were analyzed using gadolinium-EOB-DTPA and T1-weighted 3D-Flash sequences. Tumour blood flow was measured using low molecular gadobutrol and high molecular gadolinium-DTPA.</p> <p>Results</p> <p>MRI handling and applicability was similar to human systems, resolution as low as 0.1 mm. After 5 weeks tumour volumes differed significantly (p < 0.01) when comparing calliper measurments (n = 5, mean 1065 mm<sup>3</sup>+/-243 mm<sup>3</sup>) with MRI (mean 918 mm<sup>3</sup>+/-193 mm<sup>3</sup>) with MRI being more precise. Histology (n = 5) confirmed MRI tumour measurements (mean size MRI 38.5 mm<sup>2</sup>+/-22.8 mm<sup>2 </sup>versus 32.6 mm<sup>2</sup>+/-22.6 mm<sup>2 </sup>(histology), p < 0,0004) with differences due to fixation and processing of specimens. After splenic injection all mice developed liver metastases with a mean of 8 metastases and a mean volume of 173.8 mm<sup>3</sup>+/-56.7 mm<sup>3 </sup>after 5 weeks. Lymphnodes were also easily identified. Tumour accumulation of gadobutrol was significantly (p < 0.05) higher than gadolinium-DTPA. All imaging experiments could be done repeatedly to comply with the 3R-principle thus reducing the number of experimental animals.</p> <p>Conclusions</p> <p>This model permits monitoring of tumour growth and metastasis formation in longitudinal non-invasive high-resolution MR studies including using contrast agents comparable to human pancreatic cancer. This multidisciplinary environment enables radiologists, surgeons and physicians to further improve translational research and therapies of pancreatic cancer.</p> |
| first_indexed | 2024-12-18T06:29:56Z |
| format | Article |
| id | doaj.art-9939f0bfc5204a639d501b9e1ffd8cdd |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-18T06:29:56Z |
| publishDate | 2011-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-9939f0bfc5204a639d501b9e1ffd8cdd2022-12-21T21:17:56ZengBMCBMC Cancer1471-24072011-01-011114010.1186/1471-2407-11-40<it>In vivo </it>imaging of pancreatic tumours and liver metastases using 7 Tesla MRI in a murine orthotopic pancreatic cancer model and a liver metastases modelHadlich StefanLerch Markus MMayerle JuliaWeiss Ulrich FKuehn SandraDiedrich StephanSeubert FlorianRoese SebastianSpeerforck SvenKühn Jens-PAlbers NeleSendler MatthiasKaeding AndréPartecke Ivo LHosten NorbertHeidecke Claus-DPuls Ralfvon Bernstorff Wolfram<p>Abstract</p> <p>Background</p> <p>Pancreatic cancer is the fourth leading cause of tumour death in the western world. However, appropriate tumour models are scarce. Here we present a syngeneic murine pancreatic cancer model using 7 Tesla MRI and evaluate its clinical relevance and applicability.</p> <p>Methods</p> <p>6606PDA murine pancreatic cancer cells were orthotopically injected into the pancreatic head. Liver metastases were induced through splenic injection. Animals were analyzed by MRI three and five weeks following injection. Tumours were detected using T2-weighted high resolution sequences. Tumour volumes were determined by callipers and MRI. Liver metastases were analyzed using gadolinium-EOB-DTPA and T1-weighted 3D-Flash sequences. Tumour blood flow was measured using low molecular gadobutrol and high molecular gadolinium-DTPA.</p> <p>Results</p> <p>MRI handling and applicability was similar to human systems, resolution as low as 0.1 mm. After 5 weeks tumour volumes differed significantly (p < 0.01) when comparing calliper measurments (n = 5, mean 1065 mm<sup>3</sup>+/-243 mm<sup>3</sup>) with MRI (mean 918 mm<sup>3</sup>+/-193 mm<sup>3</sup>) with MRI being more precise. Histology (n = 5) confirmed MRI tumour measurements (mean size MRI 38.5 mm<sup>2</sup>+/-22.8 mm<sup>2 </sup>versus 32.6 mm<sup>2</sup>+/-22.6 mm<sup>2 </sup>(histology), p < 0,0004) with differences due to fixation and processing of specimens. After splenic injection all mice developed liver metastases with a mean of 8 metastases and a mean volume of 173.8 mm<sup>3</sup>+/-56.7 mm<sup>3 </sup>after 5 weeks. Lymphnodes were also easily identified. Tumour accumulation of gadobutrol was significantly (p < 0.05) higher than gadolinium-DTPA. All imaging experiments could be done repeatedly to comply with the 3R-principle thus reducing the number of experimental animals.</p> <p>Conclusions</p> <p>This model permits monitoring of tumour growth and metastasis formation in longitudinal non-invasive high-resolution MR studies including using contrast agents comparable to human pancreatic cancer. This multidisciplinary environment enables radiologists, surgeons and physicians to further improve translational research and therapies of pancreatic cancer.</p>http://www.biomedcentral.com/1471-2407/11/40 |
| spellingShingle | Hadlich Stefan Lerch Markus M Mayerle Julia Weiss Ulrich F Kuehn Sandra Diedrich Stephan Seubert Florian Roese Sebastian Speerforck Sven Kühn Jens-P Albers Nele Sendler Matthias Kaeding André Partecke Ivo L Hosten Norbert Heidecke Claus-D Puls Ralf von Bernstorff Wolfram <it>In vivo </it>imaging of pancreatic tumours and liver metastases using 7 Tesla MRI in a murine orthotopic pancreatic cancer model and a liver metastases model BMC Cancer |
| title | <it>In vivo </it>imaging of pancreatic tumours and liver metastases using 7 Tesla MRI in a murine orthotopic pancreatic cancer model and a liver metastases model |
| title_full | <it>In vivo </it>imaging of pancreatic tumours and liver metastases using 7 Tesla MRI in a murine orthotopic pancreatic cancer model and a liver metastases model |
| title_fullStr | <it>In vivo </it>imaging of pancreatic tumours and liver metastases using 7 Tesla MRI in a murine orthotopic pancreatic cancer model and a liver metastases model |
| title_full_unstemmed | <it>In vivo </it>imaging of pancreatic tumours and liver metastases using 7 Tesla MRI in a murine orthotopic pancreatic cancer model and a liver metastases model |
| title_short | <it>In vivo </it>imaging of pancreatic tumours and liver metastases using 7 Tesla MRI in a murine orthotopic pancreatic cancer model and a liver metastases model |
| title_sort | it in vivo it imaging of pancreatic tumours and liver metastases using 7 tesla mri in a murine orthotopic pancreatic cancer model and a liver metastases model |
| url | http://www.biomedcentral.com/1471-2407/11/40 |
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