Beta-glucan enhanced immune response to Newcastle disease vaccine and changed mRNA expression of spleen in chickens
ABSTRACT: The present study was performed to investigate the effect of oral administration of β-glucan (G70), a product obtained from the cell wall of yeast, on Newcastle disease virus (NDV)-specific hemagglutination inhibition (HI) titers, lymphocyte proliferation, and the role of T lymphocyte subp...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122007088 |
| _version_ | 1811175488826114048 |
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| author | Liting Cao Jun Li Jianrong Zhang Huan Huang Fuxing Gui Wei Xu Li Zhang Shicheng Bi |
| author_facet | Liting Cao Jun Li Jianrong Zhang Huan Huang Fuxing Gui Wei Xu Li Zhang Shicheng Bi |
| author_sort | Liting Cao |
| collection | DOAJ |
| description | ABSTRACT: The present study was performed to investigate the effect of oral administration of β-glucan (G70), a product obtained from the cell wall of yeast, on Newcastle disease virus (NDV)-specific hemagglutination inhibition (HI) titers, lymphocyte proliferation, and the role of T lymphocyte subpopulations in chickens treated with live NDV vaccine. In addition, the influence of β-glucan on splenic gene expression was investigated by transcriptome sequencing. The results revealed that the supplementation of β-glucan boosted the titer of serum NDV HI increased the NDV stimulation index of lymphocytes in peripheral blood and intestinal tract, and promoted the differentiation of T lymphocytes into CD4+ T cells. The RNA sequencing (RNA-seq) analysis demonstrated that G70 upregulated the mRNA expressions related to G-protein coupled receptor and MHC class I polypeptide, and downregulated the mRNA expressions related to cathelicidin and beta-defensin. The immunomodulatory effect of G70 might function through mitogen-activated protein kinase signaling pathway. To sum up, G70 could boost the immunological efficacy of live NDV vaccine in chickens and could be applied as a potential adjuvant candidate in the poultry industry. |
| first_indexed | 2024-04-10T19:37:52Z |
| format | Article |
| id | doaj.art-993b06374beb4ea58a018ebe2557c838 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-10T19:37:52Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-993b06374beb4ea58a018ebe2557c8382023-01-30T04:11:41ZengElsevierPoultry Science0032-57912023-02-011022102414Beta-glucan enhanced immune response to Newcastle disease vaccine and changed mRNA expression of spleen in chickensLiting Cao0Jun Li1Jianrong Zhang2Huan Huang3Fuxing Gui4Wei Xu5Li Zhang6Shicheng Bi7Department of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. ChinaDepartment of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. ChinaDepartment of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. ChinaDepartment of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. ChinaDepartment of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. ChinaZhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, MOA Key Laboratory of Animal Virology, Center for Veterinary Sciences, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. ChinaImmunology Research Center, Medical Research Institute, Southwest University, Rongchang, Chongqing 402460, P. R. ChinaDepartment of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Southwest University, Rongchang, Chongqing, 402460, P. R. China; Correspondence author:ABSTRACT: The present study was performed to investigate the effect of oral administration of β-glucan (G70), a product obtained from the cell wall of yeast, on Newcastle disease virus (NDV)-specific hemagglutination inhibition (HI) titers, lymphocyte proliferation, and the role of T lymphocyte subpopulations in chickens treated with live NDV vaccine. In addition, the influence of β-glucan on splenic gene expression was investigated by transcriptome sequencing. The results revealed that the supplementation of β-glucan boosted the titer of serum NDV HI increased the NDV stimulation index of lymphocytes in peripheral blood and intestinal tract, and promoted the differentiation of T lymphocytes into CD4+ T cells. The RNA sequencing (RNA-seq) analysis demonstrated that G70 upregulated the mRNA expressions related to G-protein coupled receptor and MHC class I polypeptide, and downregulated the mRNA expressions related to cathelicidin and beta-defensin. The immunomodulatory effect of G70 might function through mitogen-activated protein kinase signaling pathway. To sum up, G70 could boost the immunological efficacy of live NDV vaccine in chickens and could be applied as a potential adjuvant candidate in the poultry industry.http://www.sciencedirect.com/science/article/pii/S0032579122007088Newcastle disease vaccinebeta-glucanadjuvantblack-bone chickenRNA-seq |
| spellingShingle | Liting Cao Jun Li Jianrong Zhang Huan Huang Fuxing Gui Wei Xu Li Zhang Shicheng Bi Beta-glucan enhanced immune response to Newcastle disease vaccine and changed mRNA expression of spleen in chickens Poultry Science Newcastle disease vaccine beta-glucan adjuvant black-bone chicken RNA-seq |
| title | Beta-glucan enhanced immune response to Newcastle disease vaccine and changed mRNA expression of spleen in chickens |
| title_full | Beta-glucan enhanced immune response to Newcastle disease vaccine and changed mRNA expression of spleen in chickens |
| title_fullStr | Beta-glucan enhanced immune response to Newcastle disease vaccine and changed mRNA expression of spleen in chickens |
| title_full_unstemmed | Beta-glucan enhanced immune response to Newcastle disease vaccine and changed mRNA expression of spleen in chickens |
| title_short | Beta-glucan enhanced immune response to Newcastle disease vaccine and changed mRNA expression of spleen in chickens |
| title_sort | beta glucan enhanced immune response to newcastle disease vaccine and changed mrna expression of spleen in chickens |
| topic | Newcastle disease vaccine beta-glucan adjuvant black-bone chicken RNA-seq |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122007088 |
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