Protein extraction from intercellular space of banana leaves, cv. ‘Grande naine’ (<i>Musa</i> AAA)
The extraction and characterization of proteins involved in plant pathogenesis process, has been an important tool for pathosystem study. The study of secreted proteins to intercellular space of leaves in response to biotic and abiotic factors constitutes an important step to reach this purpose. Int...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2006-01-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/404 |
| _version_ | 1830286862415036416 |
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| author | Milady Mendoza-Rodríguez Aminael Sánchez Michel Leiva-Mora Mayra Acosta-Suárez Luis Rojas Elio Jiménez Orelvis Portal |
| author_facet | Milady Mendoza-Rodríguez Aminael Sánchez Michel Leiva-Mora Mayra Acosta-Suárez Luis Rojas Elio Jiménez Orelvis Portal |
| author_sort | Milady Mendoza-Rodríguez |
| collection | DOAJ |
| description | The extraction and characterization of proteins involved in plant pathogenesis process, has been an important tool for pathosystem study. The study of secreted proteins to intercellular space of leaves in response to biotic and abiotic factors constitutes an important step to reach this purpose. Intercellular proteins, from healthy and infected leaves tissue of banana plants Musa AAA (subgroup Cavendish) cultivar ‘Grande naine’ at different stages of development of the Black Sigatoka disease were extracted. Leaves fragments of 2 cm2 were infiltrated at vacuum, centrifuged and 80% (w/v) of ammonium sulphate for protein precipitation was added to the collected intercellular fluid , which were analyzed in a denaturant polyacrilamide gel at 12%. The average protein concentration obtained was 0.43 mg.ml-1 for healthy and 0.83 until 1.0 mg.ml-1 for infected samples from intercellular space. The results are the first step in Musa-Mycosphaerella fijiensis pathosystem study, allowing to know about the proteins involved in the interaction, as an essential aspect to take into account for the development of future strategies of resistance to pathogen.
Key words: denatured electrophoresis, protein purification, salt precipitation |
| first_indexed | 2024-12-19T04:05:59Z |
| format | Article |
| id | doaj.art-993d0755edbf4876a5e66fb3f21c66cc |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-19T04:05:59Z |
| publishDate | 2006-01-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-993d0755edbf4876a5e66fb3f21c66cc2022-12-21T20:36:31ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472006-01-0161374Protein extraction from intercellular space of banana leaves, cv. ‘Grande naine’ (<i>Musa</i> AAA)Milady Mendoza-RodríguezAminael SánchezMichel Leiva-MoraMayra Acosta-SuárezLuis RojasElio JiménezOrelvis PortalThe extraction and characterization of proteins involved in plant pathogenesis process, has been an important tool for pathosystem study. The study of secreted proteins to intercellular space of leaves in response to biotic and abiotic factors constitutes an important step to reach this purpose. Intercellular proteins, from healthy and infected leaves tissue of banana plants Musa AAA (subgroup Cavendish) cultivar ‘Grande naine’ at different stages of development of the Black Sigatoka disease were extracted. Leaves fragments of 2 cm2 were infiltrated at vacuum, centrifuged and 80% (w/v) of ammonium sulphate for protein precipitation was added to the collected intercellular fluid , which were analyzed in a denaturant polyacrilamide gel at 12%. The average protein concentration obtained was 0.43 mg.ml-1 for healthy and 0.83 until 1.0 mg.ml-1 for infected samples from intercellular space. The results are the first step in Musa-Mycosphaerella fijiensis pathosystem study, allowing to know about the proteins involved in the interaction, as an essential aspect to take into account for the development of future strategies of resistance to pathogen. Key words: denatured electrophoresis, protein purification, salt precipitationhttps://revista.ibp.co.cu/index.php/BV/article/view/404 |
| spellingShingle | Milady Mendoza-Rodríguez Aminael Sánchez Michel Leiva-Mora Mayra Acosta-Suárez Luis Rojas Elio Jiménez Orelvis Portal Protein extraction from intercellular space of banana leaves, cv. ‘Grande naine’ (<i>Musa</i> AAA) Biotecnología Vegetal |
| title | Protein extraction from intercellular space of banana leaves, cv. ‘Grande naine’ (<i>Musa</i> AAA) |
| title_full | Protein extraction from intercellular space of banana leaves, cv. ‘Grande naine’ (<i>Musa</i> AAA) |
| title_fullStr | Protein extraction from intercellular space of banana leaves, cv. ‘Grande naine’ (<i>Musa</i> AAA) |
| title_full_unstemmed | Protein extraction from intercellular space of banana leaves, cv. ‘Grande naine’ (<i>Musa</i> AAA) |
| title_short | Protein extraction from intercellular space of banana leaves, cv. ‘Grande naine’ (<i>Musa</i> AAA) |
| title_sort | protein extraction from intercellular space of banana leaves cv grande naine i musa i aaa |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/404 |
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