Vitamin D receptor (VDR) expression in different molecular subtypes of canine mammary carcinoma
Abstract Background The molecular-based classification of canine mammary carcinomas (CMCs) has been the focus of much current research. Both in canines and humans, the triple-negative (TN) molecular subtype of mammary cancer is defined by a lack of expression of progesterone receptor (PR), oestrogen...
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| Format: | Article |
| Language: | English |
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BMC
2021-05-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-021-02901-1 |
| _version_ | 1818648216122425344 |
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| author | R. Sánchez-Céspedes M. D. Fernández-Martínez A. I. Raya C. Pineda I. López Y. Millán |
| author_facet | R. Sánchez-Céspedes M. D. Fernández-Martínez A. I. Raya C. Pineda I. López Y. Millán |
| author_sort | R. Sánchez-Céspedes |
| collection | DOAJ |
| description | Abstract Background The molecular-based classification of canine mammary carcinomas (CMCs) has been the focus of much current research. Both in canines and humans, the triple-negative (TN) molecular subtype of mammary cancer is defined by a lack of expression of progesterone receptor (PR), oestrogen receptor (ER) and HER2. It has a poor prognosis; no effective targeted therapy is available. Vitamin D displays anticarcinogenic properties, and the expression of its receptor (VDR) has been found in different molecular subtypes, being about 30–40 % of TN breast cancer (TNBC) positive to it. We assessed the VDR expression in the different molecular subtypes of 58 CMCs from 45 female dogs using an immunohistochemical panel for the molecular classification of included: PR, ER, HER2, cytokeratin (CK) 5, CK14, and Ki67. In addition, we studied the relationship among the molecular subtypes of CMCs and clinicopathologic parameters. Results Investigation showed VDR positivity in 45.0 % of the triple-negative CMCs (TNCMCs), 27.3 % of luminal B and 19.0 % of luminal A. Luminal A was the most molecular subtype represented of the total tumours (36.2 %), followed of TNCMCs (34.5 %), luminal B (20.7 %) and HER2-overexpression (10.3 %). Both HER2-overexpression and TNCMC subtypes were positively related to lymphatic invasion (P = 0.028), simple histologic subtype (P = 0.007), a higher histological grade (P = 0.045) and a trend to higher proliferation index (P = 0.09). Conclusions The highest VDR expression was observed in TNCMC, being almost half of them (45 %) positive to this receptor. VDR expression was absent in HER2-overexpression tumours and low in luminal A and B molecular subtypes. |
| first_indexed | 2024-12-17T01:14:54Z |
| format | Article |
| id | doaj.art-99403e26632944cb800e24e95a27347e |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-17T01:14:54Z |
| publishDate | 2021-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-99403e26632944cb800e24e95a27347e2022-12-21T22:09:01ZengBMCBMC Veterinary Research1746-61482021-05-0117111410.1186/s12917-021-02901-1Vitamin D receptor (VDR) expression in different molecular subtypes of canine mammary carcinomaR. Sánchez-Céspedes0M. D. Fernández-Martínez1A. I. Raya2C. Pineda3I. López4Y. Millán5Department of Comparative Pathology, Veterinary Faculty, University of CórdobaDepartment of Comparative Pathology, Veterinary Faculty, University of CórdobaDepartment of Medicine and Animal Surgery, Veterinary Faculty, University of CórdobaDepartment of Medicine and Animal Surgery, Veterinary Faculty, University of CórdobaDepartment of Medicine and Animal Surgery, Veterinary Faculty, University of CórdobaDepartment of Comparative Pathology, Veterinary Faculty, University of CórdobaAbstract Background The molecular-based classification of canine mammary carcinomas (CMCs) has been the focus of much current research. Both in canines and humans, the triple-negative (TN) molecular subtype of mammary cancer is defined by a lack of expression of progesterone receptor (PR), oestrogen receptor (ER) and HER2. It has a poor prognosis; no effective targeted therapy is available. Vitamin D displays anticarcinogenic properties, and the expression of its receptor (VDR) has been found in different molecular subtypes, being about 30–40 % of TN breast cancer (TNBC) positive to it. We assessed the VDR expression in the different molecular subtypes of 58 CMCs from 45 female dogs using an immunohistochemical panel for the molecular classification of included: PR, ER, HER2, cytokeratin (CK) 5, CK14, and Ki67. In addition, we studied the relationship among the molecular subtypes of CMCs and clinicopathologic parameters. Results Investigation showed VDR positivity in 45.0 % of the triple-negative CMCs (TNCMCs), 27.3 % of luminal B and 19.0 % of luminal A. Luminal A was the most molecular subtype represented of the total tumours (36.2 %), followed of TNCMCs (34.5 %), luminal B (20.7 %) and HER2-overexpression (10.3 %). Both HER2-overexpression and TNCMC subtypes were positively related to lymphatic invasion (P = 0.028), simple histologic subtype (P = 0.007), a higher histological grade (P = 0.045) and a trend to higher proliferation index (P = 0.09). Conclusions The highest VDR expression was observed in TNCMC, being almost half of them (45 %) positive to this receptor. VDR expression was absent in HER2-overexpression tumours and low in luminal A and B molecular subtypes.https://doi.org/10.1186/s12917-021-02901-1DogCanine mammary tumorComparative oncologyImmunohistochemistryVitamin D 3 receptor |
| spellingShingle | R. Sánchez-Céspedes M. D. Fernández-Martínez A. I. Raya C. Pineda I. López Y. Millán Vitamin D receptor (VDR) expression in different molecular subtypes of canine mammary carcinoma BMC Veterinary Research Dog Canine mammary tumor Comparative oncology Immunohistochemistry Vitamin D 3 receptor |
| title | Vitamin D receptor (VDR) expression in different molecular subtypes of canine mammary carcinoma |
| title_full | Vitamin D receptor (VDR) expression in different molecular subtypes of canine mammary carcinoma |
| title_fullStr | Vitamin D receptor (VDR) expression in different molecular subtypes of canine mammary carcinoma |
| title_full_unstemmed | Vitamin D receptor (VDR) expression in different molecular subtypes of canine mammary carcinoma |
| title_short | Vitamin D receptor (VDR) expression in different molecular subtypes of canine mammary carcinoma |
| title_sort | vitamin d receptor vdr expression in different molecular subtypes of canine mammary carcinoma |
| topic | Dog Canine mammary tumor Comparative oncology Immunohistochemistry Vitamin D 3 receptor |
| url | https://doi.org/10.1186/s12917-021-02901-1 |
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