ZAP-70 expression is associated with increased CD4 central memory T cells in chronic lymphocytic leukemia: cross-sectional study
Background: Although chronic lymphocytic leukemia is basically a B cell disease, its pathophysiology and evolution are thought to be significantly influenced by T cells, as these are probably the most important interaction partner of neoplastic B cells, participating in their expansion, differentiat...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-10-01
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| Series: | Hematology, Transfusion and Cell Therapy |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2531137918300907 |
| _version_ | 1818676779372511232 |
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| author | Rodolfo Patussi Correia Flávia Amoroso Matos e Silva Nydia Strachman Bacal Paulo Vidal Campregher Nelson Hamerschlak Gustavo Pessini Amarante-Mendes |
| author_facet | Rodolfo Patussi Correia Flávia Amoroso Matos e Silva Nydia Strachman Bacal Paulo Vidal Campregher Nelson Hamerschlak Gustavo Pessini Amarante-Mendes |
| author_sort | Rodolfo Patussi Correia |
| collection | DOAJ |
| description | Background: Although chronic lymphocytic leukemia is basically a B cell disease, its pathophysiology and evolution are thought to be significantly influenced by T cells, as these are probably the most important interaction partner of neoplastic B cells, participating in their expansion, differentiation and survival. Chronic lymphocytic leukemia B cells may also drive functional and phenotypic changes of non-malignant T cells. There are few data about the association between memory T cells and prognosis, especially related to ZAP-70, a common reliable surrogate of the gold standard chronic lymphocytic leukemia prognostic markers. Objective: The aim of this study was to investigate whether the expression of ZAP-70 in chronic lymphocytic leukemia patients is associated with abnormal patterns of the distribution of naïve and memory T cells related to crosstalk between these cells. Methods: In this cross-sectional, controlled study, patients with chronic lymphocytic leukemia were compared with healthy blood donors regarding the expression of ZAP-70 and the distribution of naïve and memory T cell subsets in peripheral blood as measured by flow cytometry. Results: ZAP-70 positive patients presented an increased frequency and absolute number of central memory CD4+ T cells, but not CD8+ T cells, compared to ZAP-70 negative patients and age-matched apparently healthy donors. Conclusions: Because central memory CD4+ T cells are located in lymph nodes and express CD40L, we consider that malignant ZAP-70-positive B cells may receive beneficial signals from central memory CD4+ T cells as they accumulate, which could contribute to more aggressive disease. Keywords: Chronic lymphocytic leukemia, ZAP-70 protein-tyrosine kinase, Memory T cells |
| first_indexed | 2024-12-17T08:48:54Z |
| format | Article |
| id | doaj.art-994209e8abb942ef9d42db48e11d8ead |
| institution | Directory Open Access Journal |
| issn | 2531-1379 |
| language | English |
| last_indexed | 2024-12-17T08:48:54Z |
| publishDate | 2018-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Hematology, Transfusion and Cell Therapy |
| spelling | doaj.art-994209e8abb942ef9d42db48e11d8ead2022-12-21T21:56:08ZengElsevierHematology, Transfusion and Cell Therapy2531-13792018-10-01404317325ZAP-70 expression is associated with increased CD4 central memory T cells in chronic lymphocytic leukemia: cross-sectional studyRodolfo Patussi Correia0Flávia Amoroso Matos e Silva1Nydia Strachman Bacal2Paulo Vidal Campregher3Nelson Hamerschlak4Gustavo Pessini Amarante-Mendes5Instituto Israelita de Ensino e Pesquisa, São Paulo, SP, Brazil; Hospital Israelita Albert Einstein, São Paulo, SP, Brazil; Corresponding author at: Laboratory of Clinical Pathology, Flow Cytometry Division, Hospital Israelita Albert Einstein, Av. Albert Einstein, 627, Morumbi, São Paulo, SP, CEP: 05652-900, Brazil.Instituto de Ciências Biomédicas, Universidade de São Paulo (ICB USP), São Paulo, SP, BrazilHospital Israelita Albert Einstein, São Paulo, SP, Brazil; Centro de Hematologia de São Paulo (CHSP), São Paulo, SP, BrazilInstituto Israelita de Ensino e Pesquisa, São Paulo, SP, Brazil; Hospital Israelita Albert Einstein, São Paulo, SP, BrazilInstituto Israelita de Ensino e Pesquisa, São Paulo, SP, Brazil; Hospital Israelita Albert Einstein, São Paulo, SP, BrazilInstituto de Ciências Biomédicas, Universidade de São Paulo (ICB USP), São Paulo, SP, Brazil; Instituto Nacional de Ciência e Tecnologia (INCT), BrazilBackground: Although chronic lymphocytic leukemia is basically a B cell disease, its pathophysiology and evolution are thought to be significantly influenced by T cells, as these are probably the most important interaction partner of neoplastic B cells, participating in their expansion, differentiation and survival. Chronic lymphocytic leukemia B cells may also drive functional and phenotypic changes of non-malignant T cells. There are few data about the association between memory T cells and prognosis, especially related to ZAP-70, a common reliable surrogate of the gold standard chronic lymphocytic leukemia prognostic markers. Objective: The aim of this study was to investigate whether the expression of ZAP-70 in chronic lymphocytic leukemia patients is associated with abnormal patterns of the distribution of naïve and memory T cells related to crosstalk between these cells. Methods: In this cross-sectional, controlled study, patients with chronic lymphocytic leukemia were compared with healthy blood donors regarding the expression of ZAP-70 and the distribution of naïve and memory T cell subsets in peripheral blood as measured by flow cytometry. Results: ZAP-70 positive patients presented an increased frequency and absolute number of central memory CD4+ T cells, but not CD8+ T cells, compared to ZAP-70 negative patients and age-matched apparently healthy donors. Conclusions: Because central memory CD4+ T cells are located in lymph nodes and express CD40L, we consider that malignant ZAP-70-positive B cells may receive beneficial signals from central memory CD4+ T cells as they accumulate, which could contribute to more aggressive disease. Keywords: Chronic lymphocytic leukemia, ZAP-70 protein-tyrosine kinase, Memory T cellshttp://www.sciencedirect.com/science/article/pii/S2531137918300907 |
| spellingShingle | Rodolfo Patussi Correia Flávia Amoroso Matos e Silva Nydia Strachman Bacal Paulo Vidal Campregher Nelson Hamerschlak Gustavo Pessini Amarante-Mendes ZAP-70 expression is associated with increased CD4 central memory T cells in chronic lymphocytic leukemia: cross-sectional study Hematology, Transfusion and Cell Therapy |
| title | ZAP-70 expression is associated with increased CD4 central memory T cells in chronic lymphocytic leukemia: cross-sectional study |
| title_full | ZAP-70 expression is associated with increased CD4 central memory T cells in chronic lymphocytic leukemia: cross-sectional study |
| title_fullStr | ZAP-70 expression is associated with increased CD4 central memory T cells in chronic lymphocytic leukemia: cross-sectional study |
| title_full_unstemmed | ZAP-70 expression is associated with increased CD4 central memory T cells in chronic lymphocytic leukemia: cross-sectional study |
| title_short | ZAP-70 expression is associated with increased CD4 central memory T cells in chronic lymphocytic leukemia: cross-sectional study |
| title_sort | zap 70 expression is associated with increased cd4 central memory t cells in chronic lymphocytic leukemia cross sectional study |
| url | http://www.sciencedirect.com/science/article/pii/S2531137918300907 |
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