Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatica
Context: Stachys guyoniana Noë ex. Batt. and Mentha aquatica L. are two Algerian Lamiaceae used in folk medicine. Objective: To investigate their antioxidant, anticholinesterase and antibacterial activities. Material and methods: n-Butanol (BESG), ethyl acetate (EESG) and chloroform (CESG) extracts...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2016.1238488 |
| _version_ | 1818452958171365376 |
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| author | Maria Ferhat Ebru Erol Khadidja Aya Beladjila Yunus Çetintaş Mehmet Emin Duru Mehmet Öztürk Ahmed Kabouche Zahia Kabouche |
| author_facet | Maria Ferhat Ebru Erol Khadidja Aya Beladjila Yunus Çetintaş Mehmet Emin Duru Mehmet Öztürk Ahmed Kabouche Zahia Kabouche |
| author_sort | Maria Ferhat |
| collection | DOAJ |
| description | Context: Stachys guyoniana Noë ex. Batt. and Mentha aquatica L. are two Algerian Lamiaceae used in folk medicine. Objective: To investigate their antioxidant, anticholinesterase and antibacterial activities. Material and methods: n-Butanol (BESG), ethyl acetate (EESG) and chloroform (CESG) extracts of S. guyoniana and methanol (MEMA) and chloroform (CEMA) aerial part extracts of M. aquatica and methanol (MERMA) and acetone (AERMA) roots extracts of M. aquatica were evaluated for their antioxidant activity by the β-carotene-linoleic acid, DPPH• and ABTS•+ scavenging, CUPRAC and metal chelating assays. The anticholinesterase activity was tested against AChE and BChE. The antibacterial activity was assessed by MICs determination against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella heidelberg, Klebsiella pneumoniae, Enterobacter aerogenes and Morganella morganii strains. Results: In the β-carotene test, the CESG (IC50: 2.3 ± 1.27 μg/mL) exhibited the highest activity. The BESG was the best scavenger of DPPH• (IC50: 2.91 ± 0.14 μg/mL). In the ABTS test, AERMA was the most active (IC50: 4.21 ± 0.28 μg/mL). However, with the CUPRAC, the BESG exhibited the best activity (A0.50: 0.15 ± 0.05 μg/mL) and was active in metal chelating assay with 48% inhibition at 100 μg/mL. The BESG was the best AChE inhibitor (IC50: 5.78 ± 0.01 μg/mL) however, the AERMA showed the highest BChE inhibitory activity (IC50: 19.23 ± 1.42 μg/mL). The tested extracts exhibited a good antibacterial activity. Conclusion: This study demonstrated good antioxidant, anticholinesterase and antibacterial potential of S. guyoniana and M. aquatica, which fits in well with their use in folk medicine. |
| first_indexed | 2024-12-14T21:31:21Z |
| format | Article |
| id | doaj.art-99436016b0504ac6ae5a5142cb314a0a |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-14T21:31:21Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-99436016b0504ac6ae5a5142cb314a0a2022-12-21T22:46:41ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-0155132432910.1080/13880209.2016.12384881238488Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquaticaMaria Ferhat0Ebru Erol1Khadidja Aya Beladjila2Yunus Çetintaş3Mehmet Emin Duru4Mehmet Öztürk5Ahmed Kabouche6Zahia Kabouche7Université des frères Mentouri-ConstantineMugla Sıtkı Koçman UniversityUniversité des frères Mentouri-ConstantineMugla Sıtkı Koçman UniversityUniversité des frères Mentouri-ConstantineMugla Sıtkı Koçman UniversityUniversité des frères Mentouri-ConstantineUniversité des frères Mentouri-ConstantineContext: Stachys guyoniana Noë ex. Batt. and Mentha aquatica L. are two Algerian Lamiaceae used in folk medicine. Objective: To investigate their antioxidant, anticholinesterase and antibacterial activities. Material and methods: n-Butanol (BESG), ethyl acetate (EESG) and chloroform (CESG) extracts of S. guyoniana and methanol (MEMA) and chloroform (CEMA) aerial part extracts of M. aquatica and methanol (MERMA) and acetone (AERMA) roots extracts of M. aquatica were evaluated for their antioxidant activity by the β-carotene-linoleic acid, DPPH• and ABTS•+ scavenging, CUPRAC and metal chelating assays. The anticholinesterase activity was tested against AChE and BChE. The antibacterial activity was assessed by MICs determination against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella heidelberg, Klebsiella pneumoniae, Enterobacter aerogenes and Morganella morganii strains. Results: In the β-carotene test, the CESG (IC50: 2.3 ± 1.27 μg/mL) exhibited the highest activity. The BESG was the best scavenger of DPPH• (IC50: 2.91 ± 0.14 μg/mL). In the ABTS test, AERMA was the most active (IC50: 4.21 ± 0.28 μg/mL). However, with the CUPRAC, the BESG exhibited the best activity (A0.50: 0.15 ± 0.05 μg/mL) and was active in metal chelating assay with 48% inhibition at 100 μg/mL. The BESG was the best AChE inhibitor (IC50: 5.78 ± 0.01 μg/mL) however, the AERMA showed the highest BChE inhibitory activity (IC50: 19.23 ± 1.42 μg/mL). The tested extracts exhibited a good antibacterial activity. Conclusion: This study demonstrated good antioxidant, anticholinesterase and antibacterial potential of S. guyoniana and M. aquatica, which fits in well with their use in folk medicine.http://dx.doi.org/10.1080/13880209.2016.1238488dpphabts+β-carotene/linoleic acidcupracmetal chelatingcholinesterase inhibitory activityagar dilution |
| spellingShingle | Maria Ferhat Ebru Erol Khadidja Aya Beladjila Yunus Çetintaş Mehmet Emin Duru Mehmet Öztürk Ahmed Kabouche Zahia Kabouche Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatica Pharmaceutical Biology dpph abts+ β-carotene/linoleic acid cuprac metal chelating cholinesterase inhibitory activity agar dilution |
| title | Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatica |
| title_full | Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatica |
| title_fullStr | Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatica |
| title_full_unstemmed | Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatica |
| title_short | Antioxidant, anticholinesterase and antibacterial activities of Stachys guyoniana and Mentha aquatica |
| title_sort | antioxidant anticholinesterase and antibacterial activities of stachys guyoniana and mentha aquatica |
| topic | dpph abts+ β-carotene/linoleic acid cuprac metal chelating cholinesterase inhibitory activity agar dilution |
| url | http://dx.doi.org/10.1080/13880209.2016.1238488 |
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