Aggregatibacter actinomycetemcomitans GroEL protein promotes conversion of human CD4+ T cells into IFNγ IL10 producing Tbet+ Th1 cells.
One of the heat shock family protein (Hsp) expressing bacteria is the gram negative, periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa). A. actinomycetemcomitans' Hsp is a 64-kDa GroEL-protein, which has been shown to influence the host cells. In this study we used recombinant A. a...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3495765?pdf=render |
| _version_ | 1831729980887793664 |
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| author | Tahsin Saygılı Semih Can Akıncılar Bünyamin Akgül Ayten Nalbant |
| author_facet | Tahsin Saygılı Semih Can Akıncılar Bünyamin Akgül Ayten Nalbant |
| author_sort | Tahsin Saygılı |
| collection | DOAJ |
| description | One of the heat shock family protein (Hsp) expressing bacteria is the gram negative, periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa). A. actinomycetemcomitans' Hsp is a 64-kDa GroEL-protein, which has been shown to influence the host cells. In this study we used recombinant A. actinomycetemcomitans GroEL (rAaGroEL) protein as a model antigen to study GroEL-mediated T cell immune response. Human peripheral mononuclear cells (PBMCs), when stimulated with recombinant rAaGroEL, expressed early activation marker CD69 and IL-2R (CD25). CD25 and CD69 expressions were higher in CD4+ T cells compared to CD8+ T cells. rAaGroEL-responding CD4+ T cells expressed IL-10, IFNγ and TNFα cytokines. Interestingly, there were also IL-10 and IFNγ double cytokine producing CD4+ T cells. Additionally, IFNγ expressing CD4+ T cells were also T-bet positive. Altogether the results suggest that rAaGroEL protein affects CD4+ T cells to differentiate into IFNγ IL10-secreting T-bet+ Th1 cells. |
| first_indexed | 2024-12-21T09:36:59Z |
| format | Article |
| id | doaj.art-9943a53f7e3d43648546757f2ce99277 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T09:36:59Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9943a53f7e3d43648546757f2ce992772022-12-21T19:08:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e4925210.1371/journal.pone.0049252Aggregatibacter actinomycetemcomitans GroEL protein promotes conversion of human CD4+ T cells into IFNγ IL10 producing Tbet+ Th1 cells.Tahsin SaygılıSemih Can AkıncılarBünyamin AkgülAyten NalbantOne of the heat shock family protein (Hsp) expressing bacteria is the gram negative, periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa). A. actinomycetemcomitans' Hsp is a 64-kDa GroEL-protein, which has been shown to influence the host cells. In this study we used recombinant A. actinomycetemcomitans GroEL (rAaGroEL) protein as a model antigen to study GroEL-mediated T cell immune response. Human peripheral mononuclear cells (PBMCs), when stimulated with recombinant rAaGroEL, expressed early activation marker CD69 and IL-2R (CD25). CD25 and CD69 expressions were higher in CD4+ T cells compared to CD8+ T cells. rAaGroEL-responding CD4+ T cells expressed IL-10, IFNγ and TNFα cytokines. Interestingly, there were also IL-10 and IFNγ double cytokine producing CD4+ T cells. Additionally, IFNγ expressing CD4+ T cells were also T-bet positive. Altogether the results suggest that rAaGroEL protein affects CD4+ T cells to differentiate into IFNγ IL10-secreting T-bet+ Th1 cells.http://europepmc.org/articles/PMC3495765?pdf=render |
| spellingShingle | Tahsin Saygılı Semih Can Akıncılar Bünyamin Akgül Ayten Nalbant Aggregatibacter actinomycetemcomitans GroEL protein promotes conversion of human CD4+ T cells into IFNγ IL10 producing Tbet+ Th1 cells. PLoS ONE |
| title | Aggregatibacter actinomycetemcomitans GroEL protein promotes conversion of human CD4+ T cells into IFNγ IL10 producing Tbet+ Th1 cells. |
| title_full | Aggregatibacter actinomycetemcomitans GroEL protein promotes conversion of human CD4+ T cells into IFNγ IL10 producing Tbet+ Th1 cells. |
| title_fullStr | Aggregatibacter actinomycetemcomitans GroEL protein promotes conversion of human CD4+ T cells into IFNγ IL10 producing Tbet+ Th1 cells. |
| title_full_unstemmed | Aggregatibacter actinomycetemcomitans GroEL protein promotes conversion of human CD4+ T cells into IFNγ IL10 producing Tbet+ Th1 cells. |
| title_short | Aggregatibacter actinomycetemcomitans GroEL protein promotes conversion of human CD4+ T cells into IFNγ IL10 producing Tbet+ Th1 cells. |
| title_sort | aggregatibacter actinomycetemcomitans groel protein promotes conversion of human cd4 t cells into ifnγ il10 producing tbet th1 cells |
| url | http://europepmc.org/articles/PMC3495765?pdf=render |
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