Engeletin mediates antiarrhythmic effects in mice with isoproterenol-induced cardiac remodeling
Objective: Engeletin is a potent natural compound with antioxidant and anti-inflammatory properties. However, its role in cardiac remodeling remains unclear. Herein, the aim of the present study was to explore the effects of engeletin on cardiac structural and electrical remodeling and its underlyin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223002275 |
| _version_ | 1797857762213888000 |
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| author | Zhao Fang Zhebo Liu Bo Tao Xuejun Jiang |
| author_facet | Zhao Fang Zhebo Liu Bo Tao Xuejun Jiang |
| author_sort | Zhao Fang |
| collection | DOAJ |
| description | Objective: Engeletin is a potent natural compound with antioxidant and anti-inflammatory properties. However, its role in cardiac remodeling remains unclear. Herein, the aim of the present study was to explore the effects of engeletin on cardiac structural and electrical remodeling and its underlying mechanism. Methods: and results: A cardiac remodeling mice model using isoproterenol (ISO)-induced myocardial fibrosis was constructed and divided into the following four groups: control group; engeletin group; ISO group; engeletin + ISO group. Our results demonstrated that engeletin alleviated ISO-induced myocardial fibrosis and dysfunction. Moreover, engeletin significantly prolonged the QT and corrected QT (QTc) intervals, effective refractory period (ERP), and action potential duration (APD), and enhanced connexin protein 43 (Cx43) and ion channel expressions, thereby decreasing ventricular fibrillation (VF) susceptibility. Additionally, dihydroethidium staining illustrated that engeletin decreased reactive oxygen species (ROS) production. Of note, engeletin also increased the levels of superoxide dismutase and glutathione and decreased the activity of malondialdehyde and L-Glutathione oxidized. Moreover, engeletin significantly increased the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). Furthermore, in vitro administration of an Nrf2 inhibitor abolished the anti-oxidant properties of engeletin. Conclusion: Engeletin ameliorated cardiac structural and electrical remodeling, ion channel remodeling, and oxidative stress induced by ISO in mice, thereby reducing VF susceptibility. These effects may be attributed to the anti-oxidant properties of engeletin associated with the Nrf2/HO-1 pathway. |
| first_indexed | 2024-04-09T21:02:01Z |
| format | Article |
| id | doaj.art-9945db4055844fd2a691a4a5f7fb4949 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-09T21:02:01Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-9945db4055844fd2a691a4a5f7fb49492023-03-29T09:21:40ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-05-01161114439Engeletin mediates antiarrhythmic effects in mice with isoproterenol-induced cardiac remodelingZhao Fang0Zhebo Liu1Bo Tao2Xuejun Jiang3Department of Cardiology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan, PR ChinaDepartment of Cardiology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, PR ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan, PR China; Corresponding authors at: Department of Cardiology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, PR China.Department of Cardiology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan, PR China; Corresponding authors at: Department of Cardiology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, PR China.Objective: Engeletin is a potent natural compound with antioxidant and anti-inflammatory properties. However, its role in cardiac remodeling remains unclear. Herein, the aim of the present study was to explore the effects of engeletin on cardiac structural and electrical remodeling and its underlying mechanism. Methods: and results: A cardiac remodeling mice model using isoproterenol (ISO)-induced myocardial fibrosis was constructed and divided into the following four groups: control group; engeletin group; ISO group; engeletin + ISO group. Our results demonstrated that engeletin alleviated ISO-induced myocardial fibrosis and dysfunction. Moreover, engeletin significantly prolonged the QT and corrected QT (QTc) intervals, effective refractory period (ERP), and action potential duration (APD), and enhanced connexin protein 43 (Cx43) and ion channel expressions, thereby decreasing ventricular fibrillation (VF) susceptibility. Additionally, dihydroethidium staining illustrated that engeletin decreased reactive oxygen species (ROS) production. Of note, engeletin also increased the levels of superoxide dismutase and glutathione and decreased the activity of malondialdehyde and L-Glutathione oxidized. Moreover, engeletin significantly increased the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). Furthermore, in vitro administration of an Nrf2 inhibitor abolished the anti-oxidant properties of engeletin. Conclusion: Engeletin ameliorated cardiac structural and electrical remodeling, ion channel remodeling, and oxidative stress induced by ISO in mice, thereby reducing VF susceptibility. These effects may be attributed to the anti-oxidant properties of engeletin associated with the Nrf2/HO-1 pathway.http://www.sciencedirect.com/science/article/pii/S0753332223002275EngeletinCardiac remodelingOxidative stressVentricular fibrillationNrf2/HO-1 |
| spellingShingle | Zhao Fang Zhebo Liu Bo Tao Xuejun Jiang Engeletin mediates antiarrhythmic effects in mice with isoproterenol-induced cardiac remodeling Biomedicine & Pharmacotherapy Engeletin Cardiac remodeling Oxidative stress Ventricular fibrillation Nrf2/HO-1 |
| title | Engeletin mediates antiarrhythmic effects in mice with isoproterenol-induced cardiac remodeling |
| title_full | Engeletin mediates antiarrhythmic effects in mice with isoproterenol-induced cardiac remodeling |
| title_fullStr | Engeletin mediates antiarrhythmic effects in mice with isoproterenol-induced cardiac remodeling |
| title_full_unstemmed | Engeletin mediates antiarrhythmic effects in mice with isoproterenol-induced cardiac remodeling |
| title_short | Engeletin mediates antiarrhythmic effects in mice with isoproterenol-induced cardiac remodeling |
| title_sort | engeletin mediates antiarrhythmic effects in mice with isoproterenol induced cardiac remodeling |
| topic | Engeletin Cardiac remodeling Oxidative stress Ventricular fibrillation Nrf2/HO-1 |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223002275 |
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