An evaluation of the anti-neoplastic activity of curcumin in prostate cancer cell lines
OBJECTIVE: The aim of our study is to investigate the anti-neoplastic effect of curcumin in prostate cancer cell lines. Specifically, we are using the LNCaP cell line and another prostate cell line developed in our laboratory, PcBra1. The PcBra1 cells were derived from a localized, obstructive prost...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Urologia
2009-06-01
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| Series: | International Brazilian Journal of Urology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382009000300012 |
| _version_ | 1811342910296162304 |
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| author | Camila B. Piantino Fernanda A. Salvadori Pedro P. Ayres Raphael B. Kato Victor Srougi Katia R. Leite Miguel Srougi |
| author_facet | Camila B. Piantino Fernanda A. Salvadori Pedro P. Ayres Raphael B. Kato Victor Srougi Katia R. Leite Miguel Srougi |
| author_sort | Camila B. Piantino |
| collection | DOAJ |
| description | OBJECTIVE: The aim of our study is to investigate the anti-neoplastic effect of curcumin in prostate cancer cell lines. Specifically, we are using the LNCaP cell line and another prostate cell line developed in our laboratory, PcBra1. The PcBra1 cells were derived from a localized, obstructive prostate cancer with a Gleason score of 9 (4+5). MATERIAL AND METHODS: A prostate cancer cell line was isolated from a localized, obstructive prostate cancer with a Gleason score of 9 (4+5), and it was characterized using immunohistochemistry. After six passages, the new cell line was treated with varying doses of curcumin: 10 µM, 25 µM or 50 µM. Apoptosis was detected by flow cytometry using Annexin V FITC. For comparison, the same experiment was performed using the well-established metastatic prostate cancer cell line, LNCaP. RESULTS: Increasing concentrations of curcumin promoted more apoptosis in the PcBra1 cells. Exposure to 10 and 25 µM curcumin induced apoptosis in 31.9% and 52.2% of cells, respectively. Late apoptosis was induced in 37% of cells after treatment with 10 µM curcumin and 35% of cells with a 25 µM treatment. Necrosis accounted for less than 10% of the death in these cells at those two concentrations. When curcumin was used at 50 µM, apoptosis was observed in 64.3% of the cells. Including late apoptosis and necrosis, 98.6% of the cells died in response to 50 µM curcumin. Results with the LNCaP cells were similar although late apoptosis was the main phenomenon at 25 µM. CONCLUSION: We have shown that curcumin acts on localized prostate cancer to induce apoptosis and may therefore be an option as a future therapeutic agent. |
| first_indexed | 2024-04-13T19:20:28Z |
| format | Article |
| id | doaj.art-9948efb3327d49bcae2b76ee5b236813 |
| institution | Directory Open Access Journal |
| issn | 1677-5538 1677-6119 |
| language | English |
| last_indexed | 2024-04-13T19:20:28Z |
| publishDate | 2009-06-01 |
| publisher | Sociedade Brasileira de Urologia |
| record_format | Article |
| series | International Brazilian Journal of Urology |
| spelling | doaj.art-9948efb3327d49bcae2b76ee5b2368132022-12-22T02:33:34ZengSociedade Brasileira de UrologiaInternational Brazilian Journal of Urology1677-55381677-61192009-06-0135335436110.1590/S1677-55382009000300012An evaluation of the anti-neoplastic activity of curcumin in prostate cancer cell linesCamila B. PiantinoFernanda A. SalvadoriPedro P. AyresRaphael B. KatoVictor SrougiKatia R. LeiteMiguel SrougiOBJECTIVE: The aim of our study is to investigate the anti-neoplastic effect of curcumin in prostate cancer cell lines. Specifically, we are using the LNCaP cell line and another prostate cell line developed in our laboratory, PcBra1. The PcBra1 cells were derived from a localized, obstructive prostate cancer with a Gleason score of 9 (4+5). MATERIAL AND METHODS: A prostate cancer cell line was isolated from a localized, obstructive prostate cancer with a Gleason score of 9 (4+5), and it was characterized using immunohistochemistry. After six passages, the new cell line was treated with varying doses of curcumin: 10 µM, 25 µM or 50 µM. Apoptosis was detected by flow cytometry using Annexin V FITC. For comparison, the same experiment was performed using the well-established metastatic prostate cancer cell line, LNCaP. RESULTS: Increasing concentrations of curcumin promoted more apoptosis in the PcBra1 cells. Exposure to 10 and 25 µM curcumin induced apoptosis in 31.9% and 52.2% of cells, respectively. Late apoptosis was induced in 37% of cells after treatment with 10 µM curcumin and 35% of cells with a 25 µM treatment. Necrosis accounted for less than 10% of the death in these cells at those two concentrations. When curcumin was used at 50 µM, apoptosis was observed in 64.3% of the cells. Including late apoptosis and necrosis, 98.6% of the cells died in response to 50 µM curcumin. Results with the LNCaP cells were similar although late apoptosis was the main phenomenon at 25 µM. CONCLUSION: We have shown that curcumin acts on localized prostate cancer to induce apoptosis and may therefore be an option as a future therapeutic agent.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382009000300012curcumincurcuma longaprostate cancerapoptosis |
| spellingShingle | Camila B. Piantino Fernanda A. Salvadori Pedro P. Ayres Raphael B. Kato Victor Srougi Katia R. Leite Miguel Srougi An evaluation of the anti-neoplastic activity of curcumin in prostate cancer cell lines International Brazilian Journal of Urology curcumin curcuma longa prostate cancer apoptosis |
| title | An evaluation of the anti-neoplastic activity of curcumin in prostate cancer cell lines |
| title_full | An evaluation of the anti-neoplastic activity of curcumin in prostate cancer cell lines |
| title_fullStr | An evaluation of the anti-neoplastic activity of curcumin in prostate cancer cell lines |
| title_full_unstemmed | An evaluation of the anti-neoplastic activity of curcumin in prostate cancer cell lines |
| title_short | An evaluation of the anti-neoplastic activity of curcumin in prostate cancer cell lines |
| title_sort | evaluation of the anti neoplastic activity of curcumin in prostate cancer cell lines |
| topic | curcumin curcuma longa prostate cancer apoptosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382009000300012 |
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