SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo
Understanding whether SARS-CoV-2 could infect cells and tissues handled during ART is crucial for risk mitigation, especially during the implantation window when either endometrial biopsies are often practiced for endometrial receptivity assessment or embryo transfer is performed. To address this qu...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/2075-1729/11/12/1378 |
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| author | Delphine Haouzi Frida Entezami Edward Tuaillon Anna Gala Alice Ferrières-Hoa Sophie Brouillet Alain R. Thierry Samir Hamamah |
| author_facet | Delphine Haouzi Frida Entezami Edward Tuaillon Anna Gala Alice Ferrières-Hoa Sophie Brouillet Alain R. Thierry Samir Hamamah |
| author_sort | Delphine Haouzi |
| collection | DOAJ |
| description | Understanding whether SARS-CoV-2 could infect cells and tissues handled during ART is crucial for risk mitigation, especially during the implantation window when either endometrial biopsies are often practiced for endometrial receptivity assessment or embryo transfer is performed. To address this question, this review analyzed current knowledge of the field and retrospectively examined the gene expression profiles of SARS-CoV-2-associated receptors and proteases in a cohort of ART candidates using our previous Affymetrix microarray data. Human endometrial tissue under natural and controlled ovarian stimulation cycles and preimplantation embryos were analyzed. A focus was particularly drawn on the renin-angiotensin system, which plays a prominent role in the virus infection, and we compared the gene expression levels of receptors and proteases related to SARS-CoV-2 infection in the samples. High prevalence of genes related to the <i>ACE2</i> pathway during both cycle phases and mainly during the mid-secretory phase for <i>ACE2</i> were reported. The impact of COS protocols on endometrial gene expression profile of SARS-CoV-2-associated receptors and proteases is minimal, suggesting no additional potential risks during stimulated ART procedure. In blastocysts, <i>ACE2</i>, <i>BSG</i>, <i>CTSL</i>, <i>CTSA</i> and <i>FURIN</i> were detectable in the entire cohort at high expression level. Specimens from female genital tract should be considered as potential targets for SARS-CoV-2, especially during the implantation window. |
| first_indexed | 2024-03-10T03:43:10Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T03:43:10Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-994a45fd66ec495d88269d41843396ea2023-11-23T09:14:36ZengMDPI AGLife2075-17292021-12-011112137810.3390/life11121378SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation EmbryoDelphine Haouzi0Frida Entezami1Edward Tuaillon2Anna Gala3Alice Ferrières-Hoa4Sophie Brouillet5Alain R. Thierry6Samir Hamamah7Univ Montpellier, INSERM U1203, DEFE, 34295 Montpellier, FranceUniv Montpellier, INSERM U1203, DEFE, 34295 Montpellier, FranceCHU Montpellier, Bacteriology-Virology Department, 34295 Montpellier, FranceUniv Montpellier, INSERM U1203, DEFE, 34295 Montpellier, FranceUniv Montpellier, INSERM U1203, DEFE, 34295 Montpellier, FranceUniv Montpellier, INSERM U1203, DEFE, 34295 Montpellier, FranceRegional Institute of Cancer of Montpellier, 34090 Montpellier, FranceUniv Montpellier, INSERM U1203, DEFE, 34295 Montpellier, FranceUnderstanding whether SARS-CoV-2 could infect cells and tissues handled during ART is crucial for risk mitigation, especially during the implantation window when either endometrial biopsies are often practiced for endometrial receptivity assessment or embryo transfer is performed. To address this question, this review analyzed current knowledge of the field and retrospectively examined the gene expression profiles of SARS-CoV-2-associated receptors and proteases in a cohort of ART candidates using our previous Affymetrix microarray data. Human endometrial tissue under natural and controlled ovarian stimulation cycles and preimplantation embryos were analyzed. A focus was particularly drawn on the renin-angiotensin system, which plays a prominent role in the virus infection, and we compared the gene expression levels of receptors and proteases related to SARS-CoV-2 infection in the samples. High prevalence of genes related to the <i>ACE2</i> pathway during both cycle phases and mainly during the mid-secretory phase for <i>ACE2</i> were reported. The impact of COS protocols on endometrial gene expression profile of SARS-CoV-2-associated receptors and proteases is minimal, suggesting no additional potential risks during stimulated ART procedure. In blastocysts, <i>ACE2</i>, <i>BSG</i>, <i>CTSL</i>, <i>CTSA</i> and <i>FURIN</i> were detectable in the entire cohort at high expression level. Specimens from female genital tract should be considered as potential targets for SARS-CoV-2, especially during the implantation window.https://www.mdpi.com/2075-1729/11/12/1378SARS-CoV-2humanendometrial receptivitypreimplantation embryos |
| spellingShingle | Delphine Haouzi Frida Entezami Edward Tuaillon Anna Gala Alice Ferrières-Hoa Sophie Brouillet Alain R. Thierry Samir Hamamah SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo Life SARS-CoV-2 human endometrial receptivity preimplantation embryos |
| title | SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo |
| title_full | SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo |
| title_fullStr | SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo |
| title_full_unstemmed | SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo |
| title_short | SARS-CoV-2 and Implantation Window: Gene Expression Mapping of Human Endometrium and Preimplantation Embryo |
| title_sort | sars cov 2 and implantation window gene expression mapping of human endometrium and preimplantation embryo |
| topic | SARS-CoV-2 human endometrial receptivity preimplantation embryos |
| url | https://www.mdpi.com/2075-1729/11/12/1378 |
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