Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231
Metabolic reprogramming in tumor cells is considered one of the hallmarks of cancer. Many studies have been carried out in order to elucidate the effects of tumor cell metabolism on invasion and tumor progression. However, little is known about the immediate substrate preference in tumor cells. In t...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-01-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/9/1/16 |
| _version_ | 1797728502246539264 |
|---|---|
| author | Mª Carmen Ocaña Beatriz Martínez-Poveda Ana R. Quesada Miguel Ángel Medina |
| author_facet | Mª Carmen Ocaña Beatriz Martínez-Poveda Ana R. Quesada Miguel Ángel Medina |
| author_sort | Mª Carmen Ocaña |
| collection | DOAJ |
| description | Metabolic reprogramming in tumor cells is considered one of the hallmarks of cancer. Many studies have been carried out in order to elucidate the effects of tumor cell metabolism on invasion and tumor progression. However, little is known about the immediate substrate preference in tumor cells. In this work, we wanted to study this short-time preference using the highly invasive, hormone independent breast cancer cell line MDA-MB-231. By means of Seahorse and uptake experiments, our results point to a preference for glucose. However, although both glucose and glutamine are required for tumor cell proliferation, MDA-MB-231 cells can survive two days in the absence of glucose, but not in the absence of glutamine. On the other hand, the presence of glucose increased palmitate uptake in this cell line, which accumulates in the cytosol instead of going to the plasma membrane. In order to exert this effect, glucose needs to be converted to glycerol-3 phosphate, leading to palmitate metabolism through lipid synthesis, most likely to the synthesis of triacylglycerides. The effect of glucose on the palmitate uptake was also found in other triple-negative, invasive breast cancer cell lines, but not in the non-invasive ones. The results presented in this work suggest an important and specific role of glucose in lipid biosynthesis in triple-negative breast cancer. |
| first_indexed | 2024-03-12T11:14:57Z |
| format | Article |
| id | doaj.art-994b47f567bf44e79ecbc83a1b47b5b9 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-12T11:14:57Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-994b47f567bf44e79ecbc83a1b47b5b92023-09-02T02:17:52ZengMDPI AGBiology2079-77372020-01-01911610.3390/biology9010016biology9010016Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231Mª Carmen Ocaña0Beatriz Martínez-Poveda1Ana R. Quesada2Miguel Ángel Medina3Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Andalucía Tech, Universidad de Málaga, E-29071 Málaga, SpainDepartamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Andalucía Tech, Universidad de Málaga, E-29071 Málaga, SpainDepartamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Andalucía Tech, Universidad de Málaga, E-29071 Málaga, SpainDepartamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Andalucía Tech, Universidad de Málaga, E-29071 Málaga, SpainMetabolic reprogramming in tumor cells is considered one of the hallmarks of cancer. Many studies have been carried out in order to elucidate the effects of tumor cell metabolism on invasion and tumor progression. However, little is known about the immediate substrate preference in tumor cells. In this work, we wanted to study this short-time preference using the highly invasive, hormone independent breast cancer cell line MDA-MB-231. By means of Seahorse and uptake experiments, our results point to a preference for glucose. However, although both glucose and glutamine are required for tumor cell proliferation, MDA-MB-231 cells can survive two days in the absence of glucose, but not in the absence of glutamine. On the other hand, the presence of glucose increased palmitate uptake in this cell line, which accumulates in the cytosol instead of going to the plasma membrane. In order to exert this effect, glucose needs to be converted to glycerol-3 phosphate, leading to palmitate metabolism through lipid synthesis, most likely to the synthesis of triacylglycerides. The effect of glucose on the palmitate uptake was also found in other triple-negative, invasive breast cancer cell lines, but not in the non-invasive ones. The results presented in this work suggest an important and specific role of glucose in lipid biosynthesis in triple-negative breast cancer.https://www.mdpi.com/2079-7737/9/1/16cancerlipid metabolismglucose metabolismglutamine metabolism |
| spellingShingle | Mª Carmen Ocaña Beatriz Martínez-Poveda Ana R. Quesada Miguel Ángel Medina Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231 Biology cancer lipid metabolism glucose metabolism glutamine metabolism |
| title | Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231 |
| title_full | Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231 |
| title_fullStr | Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231 |
| title_full_unstemmed | Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231 |
| title_short | Glucose Favors Lipid Anabolic Metabolism in the Invasive Breast Cancer Cell Line MDA-MB-231 |
| title_sort | glucose favors lipid anabolic metabolism in the invasive breast cancer cell line mda mb 231 |
| topic | cancer lipid metabolism glucose metabolism glutamine metabolism |
| url | https://www.mdpi.com/2079-7737/9/1/16 |
| work_keys_str_mv | AT macarmenocana glucosefavorslipidanabolicmetabolismintheinvasivebreastcancercelllinemdamb231 AT beatrizmartinezpoveda glucosefavorslipidanabolicmetabolismintheinvasivebreastcancercelllinemdamb231 AT anarquesada glucosefavorslipidanabolicmetabolismintheinvasivebreastcancercelllinemdamb231 AT miguelangelmedina glucosefavorslipidanabolicmetabolismintheinvasivebreastcancercelllinemdamb231 |