Microbiota DNA isolation, 16S rRNA amplicon sequencing, and bioinformatic analysis for bacterial microbiome profiling of rodent fecal samples

Microbiota DNA isolation, 16S rRNA amplicon sequencing, and bioinformatic analysis for bacterial microbiome profiling of rodent fecal samples

Summary: Fecal samples are frequently used to characterize bacterial populations of the gastrointestinal tract. A protocol is provided to profile gut bacterial populations using rodent fecal samples. We describe the optimal procedures for collecting rodent fecal samples, isolating genomic DNA, 16S r...

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Bibliographic Details
Main Authors:	Chloe J. Love, Carolina Gubert, Saritha Kodikara, Geraldine Kong, Kim-Anh Lê Cao, Anthony J. Hannan
Format:	Article
Language:	English
Published:	Elsevier 2022-12-01
Series:	STAR Protocols
Subjects:	Bioinformatics Single Cell Cancer Health Sciences Genetics Genomics
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722006529

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