Chrysin 7‐O‐β‐d‐glucopyranoside increases hepatic low‐density lipoprotein receptor expression through AMP‐activated protein kinase activation
Elevated plasma low‐density lipoprotein (LDL) cholesterol level is a risk factor for developing atherosclerosis. Increased LDL receptor (LDLR) expression is expected to reduce the risk of atherosclerotic disease since hepatic LDLR is essential for clearing plasma LDL cholesterol. Here, we screened h...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-08-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13665 |
| _version_ | 1797767343501213696 |
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| author | Naoki Kaname Takahiro Fujimaki Shotaro Horikoshi Kaito Fujimura Manami Kodaka Shinnosuke Wakamori Ryo Katsuta Ken Ishigami Tsukasa Suzuki Yuji Yamamoto Jun Inoue |
| author_facet | Naoki Kaname Takahiro Fujimaki Shotaro Horikoshi Kaito Fujimura Manami Kodaka Shinnosuke Wakamori Ryo Katsuta Ken Ishigami Tsukasa Suzuki Yuji Yamamoto Jun Inoue |
| author_sort | Naoki Kaname |
| collection | DOAJ |
| description | Elevated plasma low‐density lipoprotein (LDL) cholesterol level is a risk factor for developing atherosclerosis. Increased LDL receptor (LDLR) expression is expected to reduce the risk of atherosclerotic disease since hepatic LDLR is essential for clearing plasma LDL cholesterol. Here, we screened human LDLR promoter effectors and observed that extracts from peduncles of sweet cherry (Prunus avium) ‘Sato‐Nishiki’ induce LDLR gene promoter activity. We used several analytical and chemical methods to show that chrysin 7‐O‐β‐d‐glucopyranoside (chrysin‐7G) is one of the compounds that stimulate LDLR gene promoter activity in cherry peduncle extracts. Furthermore, synthetic chrysin‐7G increased the expression and activity of LDLR. The chrysin‐7G–mediated increase in LDLR expression and activity was completely abolished by treatment with an AMP‐activated protein kinase (AMPK) inhibitor, compound C. These results indicate that chrysin‐7G increases LDLR expression through AMPK activation and may be a useful compound that can be recycled from waste parts of agricultural products. |
| first_indexed | 2024-03-12T20:38:20Z |
| format | Article |
| id | doaj.art-994be1a1443d44779258cce88d130033 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-12T20:38:20Z |
| publishDate | 2023-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-994be1a1443d44779258cce88d1300332023-08-01T12:00:58ZengWileyFEBS Open Bio2211-54632023-08-011381447145810.1002/2211-5463.13665Chrysin 7‐O‐β‐d‐glucopyranoside increases hepatic low‐density lipoprotein receptor expression through AMP‐activated protein kinase activationNaoki Kaname0Takahiro Fujimaki1Shotaro Horikoshi2Kaito Fujimura3Manami Kodaka4Shinnosuke Wakamori5Ryo Katsuta6Ken Ishigami7Tsukasa Suzuki8Yuji Yamamoto9Jun Inoue10Department of Agricultural Chemistry, Faculty of Applied Biosciences Tokyo University of Agriculture JapanDepartment of Agricultural Chemistry, Faculty of Applied Biosciences Tokyo University of Agriculture JapanDepartment of Agricultural Chemistry, Faculty of Applied Biosciences Tokyo University of Agriculture JapanDepartment of Chemistry for Life Sciences and Agriculture, Faculty of Life Sciences Tokyo University of Agriculture JapanDepartment of Agricultural Chemistry, Faculty of Applied Biosciences Tokyo University of Agriculture JapanDepartment of Chemistry for Life Sciences and Agriculture, Faculty of Life Sciences Tokyo University of Agriculture JapanDepartment of Chemistry for Life Sciences and Agriculture, Faculty of Life Sciences Tokyo University of Agriculture JapanDepartment of Chemistry for Life Sciences and Agriculture, Faculty of Life Sciences Tokyo University of Agriculture JapanDepartment of Agricultural Chemistry, Faculty of Applied Biosciences Tokyo University of Agriculture JapanDepartment of Agricultural Chemistry, Faculty of Applied Biosciences Tokyo University of Agriculture JapanDepartment of Agricultural Chemistry, Faculty of Applied Biosciences Tokyo University of Agriculture JapanElevated plasma low‐density lipoprotein (LDL) cholesterol level is a risk factor for developing atherosclerosis. Increased LDL receptor (LDLR) expression is expected to reduce the risk of atherosclerotic disease since hepatic LDLR is essential for clearing plasma LDL cholesterol. Here, we screened human LDLR promoter effectors and observed that extracts from peduncles of sweet cherry (Prunus avium) ‘Sato‐Nishiki’ induce LDLR gene promoter activity. We used several analytical and chemical methods to show that chrysin 7‐O‐β‐d‐glucopyranoside (chrysin‐7G) is one of the compounds that stimulate LDLR gene promoter activity in cherry peduncle extracts. Furthermore, synthetic chrysin‐7G increased the expression and activity of LDLR. The chrysin‐7G–mediated increase in LDLR expression and activity was completely abolished by treatment with an AMP‐activated protein kinase (AMPK) inhibitor, compound C. These results indicate that chrysin‐7G increases LDLR expression through AMPK activation and may be a useful compound that can be recycled from waste parts of agricultural products.https://doi.org/10.1002/2211-5463.13665AMPKchrysinLDL receptorpolyphenolssweet cherry |
| spellingShingle | Naoki Kaname Takahiro Fujimaki Shotaro Horikoshi Kaito Fujimura Manami Kodaka Shinnosuke Wakamori Ryo Katsuta Ken Ishigami Tsukasa Suzuki Yuji Yamamoto Jun Inoue Chrysin 7‐O‐β‐d‐glucopyranoside increases hepatic low‐density lipoprotein receptor expression through AMP‐activated protein kinase activation FEBS Open Bio AMPK chrysin LDL receptor polyphenols sweet cherry |
| title | Chrysin 7‐O‐β‐d‐glucopyranoside increases hepatic low‐density lipoprotein receptor expression through AMP‐activated protein kinase activation |
| title_full | Chrysin 7‐O‐β‐d‐glucopyranoside increases hepatic low‐density lipoprotein receptor expression through AMP‐activated protein kinase activation |
| title_fullStr | Chrysin 7‐O‐β‐d‐glucopyranoside increases hepatic low‐density lipoprotein receptor expression through AMP‐activated protein kinase activation |
| title_full_unstemmed | Chrysin 7‐O‐β‐d‐glucopyranoside increases hepatic low‐density lipoprotein receptor expression through AMP‐activated protein kinase activation |
| title_short | Chrysin 7‐O‐β‐d‐glucopyranoside increases hepatic low‐density lipoprotein receptor expression through AMP‐activated protein kinase activation |
| title_sort | chrysin 7 o β d glucopyranoside increases hepatic low density lipoprotein receptor expression through amp activated protein kinase activation |
| topic | AMPK chrysin LDL receptor polyphenols sweet cherry |
| url | https://doi.org/10.1002/2211-5463.13665 |
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