Microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows
Abstract This study aimed to explore the effects of artemisinin (ART) on the milk microbiome and metabolites of dairy cow. A total of 12 mid-lactation Holstein dairy cows with similar parity, days in milk were randomly divided into 2 groups receiving either a total mixed ration (TMR) as the control...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-08-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-020-01080-w |
| _version_ | 1818449308110815232 |
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| author | Kun Hou Jinjin Tong Hua Zhang Shan Gao Yuqin Guo Hui Niu Benhai Xiong Linshu Jiang |
| author_facet | Kun Hou Jinjin Tong Hua Zhang Shan Gao Yuqin Guo Hui Niu Benhai Xiong Linshu Jiang |
| author_sort | Kun Hou |
| collection | DOAJ |
| description | Abstract This study aimed to explore the effects of artemisinin (ART) on the milk microbiome and metabolites of dairy cow. A total of 12 mid-lactation Holstein dairy cows with similar parity, days in milk were randomly divided into 2 groups receiving either a total mixed ration (TMR) as the control group or this TMR and 120 g/d/head ART as the ART group. The milk samples were collected weekly to determine the contents, and end-of-trial (week 8) milk samples were used to identify microbial species and metabolite profiles by 16S rRNA sequencing and LC–MS analyses, respectively. We observed that the milk fat content significantly increased by ART treatment (P < 0.05). The bacterial community richness was significantly lower in the ART group (P < 0.05), while the diversity showed no difference (P > 0.05). Compared with its abundance in the control (CON) group, Firmicutes was significantly decreased, whereas Proteobacteria was significantly increased. Furthermore, in the ART group, the relative abundances of the genera Aerococcus, Staphylococcus, Corynebacterium_1 and Facklamia were significantly lower (P < 0.01). Metabolomics analysis revealed that ART significantly increasing the concentrations of glycerophospholipids, glycerolipids and flavonoids compared with those in the CON group. An enrichment analysis of the different metabolites showed that ART mainly affected glycerophospholipid metabolism and the pantothenate and CoA biosynthesis pathways. These findings revealed that ART supplementation could affect the milk microbiota and metabolites, that glycerophospholipids and glycerolipids could be potential biomarkers in the milk response to ART feed in dairy cows, and that ART changes substances in milk by maintaining lipid metabolism in the mammary gland. |
| first_indexed | 2024-12-14T20:33:20Z |
| format | Article |
| id | doaj.art-994c25dd5f694c79850886f14c1e4c93 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-14T20:33:20Z |
| publishDate | 2020-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-994c25dd5f694c79850886f14c1e4c932022-12-21T22:48:28ZengSpringerOpenAMB Express2191-08552020-08-0110111410.1186/s13568-020-01080-wMicrobiome and metabolic changes in milk in response to artemisinin supplementation in dairy cowsKun Hou0Jinjin Tong1Hua Zhang2Shan Gao3Yuqin Guo4Hui Niu5Benhai Xiong6Linshu Jiang7Beijing Key Laboratory for Dairy Cow Nutrition, Beijing University of AgricultureBeijing Key Laboratory for Dairy Cow Nutrition, Beijing University of AgricultureBeijing Key Laboratory for Dairy Cow Nutrition, Beijing University of AgricultureBeijing Key Laboratory for Dairy Cow Nutrition, Beijing University of AgricultureBeijing Key Laboratory for Dairy Cow Nutrition, Beijing University of AgricultureBeijing Key Laboratory for Dairy Cow Nutrition, Beijing University of AgricultureState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural SciencesBeijing Key Laboratory for Dairy Cow Nutrition, Beijing University of AgricultureAbstract This study aimed to explore the effects of artemisinin (ART) on the milk microbiome and metabolites of dairy cow. A total of 12 mid-lactation Holstein dairy cows with similar parity, days in milk were randomly divided into 2 groups receiving either a total mixed ration (TMR) as the control group or this TMR and 120 g/d/head ART as the ART group. The milk samples were collected weekly to determine the contents, and end-of-trial (week 8) milk samples were used to identify microbial species and metabolite profiles by 16S rRNA sequencing and LC–MS analyses, respectively. We observed that the milk fat content significantly increased by ART treatment (P < 0.05). The bacterial community richness was significantly lower in the ART group (P < 0.05), while the diversity showed no difference (P > 0.05). Compared with its abundance in the control (CON) group, Firmicutes was significantly decreased, whereas Proteobacteria was significantly increased. Furthermore, in the ART group, the relative abundances of the genera Aerococcus, Staphylococcus, Corynebacterium_1 and Facklamia were significantly lower (P < 0.01). Metabolomics analysis revealed that ART significantly increasing the concentrations of glycerophospholipids, glycerolipids and flavonoids compared with those in the CON group. An enrichment analysis of the different metabolites showed that ART mainly affected glycerophospholipid metabolism and the pantothenate and CoA biosynthesis pathways. These findings revealed that ART supplementation could affect the milk microbiota and metabolites, that glycerophospholipids and glycerolipids could be potential biomarkers in the milk response to ART feed in dairy cows, and that ART changes substances in milk by maintaining lipid metabolism in the mammary gland.http://link.springer.com/article/10.1186/s13568-020-01080-wMicrobiomeMetabolomicsArtemisininMilkDairy cows |
| spellingShingle | Kun Hou Jinjin Tong Hua Zhang Shan Gao Yuqin Guo Hui Niu Benhai Xiong Linshu Jiang Microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows AMB Express Microbiome Metabolomics Artemisinin Milk Dairy cows |
| title | Microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows |
| title_full | Microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows |
| title_fullStr | Microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows |
| title_full_unstemmed | Microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows |
| title_short | Microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows |
| title_sort | microbiome and metabolic changes in milk in response to artemisinin supplementation in dairy cows |
| topic | Microbiome Metabolomics Artemisinin Milk Dairy cows |
| url | http://link.springer.com/article/10.1186/s13568-020-01080-w |
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