Epidemiological characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic analysis of Aeromonas caviae isolated from extra-intestinal infections
ObjectiveAeromonas caviae (A. caviae) is one of the major etiological agents in human intestinal infections reported to be associated with a broad spectrum of extra-intestinal infections with increasing incidence over recent years. Although previous studies have established its significance as a ca...
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1084352/full |
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| author | Yang Song Li-feng Wang Kuai Zhou Shuang Liu Ling Guo Li-yan Ye Jiang Gu Yan Cheng Ding-xia Shen Ding-xia Shen |
| author_facet | Yang Song Li-feng Wang Kuai Zhou Shuang Liu Ling Guo Li-yan Ye Jiang Gu Yan Cheng Ding-xia Shen Ding-xia Shen |
| author_sort | Yang Song |
| collection | DOAJ |
| description | ObjectiveAeromonas caviae (A. caviae) is one of the major etiological agents in human intestinal infections reported to be associated with a broad spectrum of extra-intestinal infections with increasing incidence over recent years. Although previous studies have established its significance as a causative agent of both bloodstream and gastrointestinal infections, the characteristics of A. caviae that cause extra-intestinal infections remain unilluminated.In this single-center retrospective study, we investigated epidemiological characteristics, antimicrobial resistance genes and phenotypes, virulence genes, and phyloevolution of 47 clinical A. caviae isolated from patients with extra-intestinal infections from 2017 to 2020.MethodsA. caviae strains were identified by biochemical tests and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS), ultimately confirmed to species level by whole-genome sequencing (WGS). Antimicrobial resistance and virulence genes were identified using the Comprehensive Antibiotic Resistance Database (CARD) and the virulence factor database (VFDB), respectively. Phylogenetic analysis of 47 clinical strains was performed by combining with 521 A. caviae strains from NCBI database.ResultsA. caviae was an opportunistic pathogen in immunocompromised patients, especially those with underlying hepatobiliary diseases and malignancies. 19 out of 47 isolates were identified as multidrug resistance (MDR) strains. Piperacillin-tazobactam, levofloxacin, gentamicin, amikacin with a resistance rate of less than 10% remained as options to treat extra-intestinal infections. 24 out of 47 isolates exhibited non-susceptibility to cephalosporins and cephamycins, all of which carried β-lactamase gene, including blaMOX, blaPER-3, blaOXA, blaNDM, and blaCphA. Most stains (98%, 46/47) carried at least one of the virulence genes, but extra-intestinal infections had a low mortality rate. Phylogenetic analysis indicated the risk of nosocomial transmission but revealed no outbreak. However, the emergence of MDR and β-lactamase resistance genes in extra-intestinal isolates of A. caviae is becoming an increasing risk to public health and requires attention.ConclusionsThis study strengthen our understanding of A.caviae isolated from extra-intestinal infections. It may contribute to the management of extra-intestinal infections as well as the prevention and control of drug resistance. |
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| id | doaj.art-994cb12e066f4595b7ece1b6a0a61b48 |
| institution | Directory Open Access Journal |
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| last_indexed | 2024-04-10T07:25:20Z |
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| spelling | doaj.art-994cb12e066f4595b7ece1b6a0a61b482023-02-24T07:57:41ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-02-011310.3389/fcimb.2023.10843521084352Epidemiological characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic analysis of Aeromonas caviae isolated from extra-intestinal infectionsYang Song0Li-feng Wang1Kuai Zhou2Shuang Liu3Ling Guo4Li-yan Ye5Jiang Gu6Yan Cheng7Ding-xia Shen8Ding-xia Shen9Department of Clinical Laboratory, Medical School of Chinese PLA, Beijing, ChinaDepartment of Clinical Laboratory, First Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Clinical Laboratory, Xuanhan People’s Hospital, Xuanhan, Sichuan, ChinaDepartment of Clinical Laboratory, Hainan Modern Women and Children’s Hospital, Haikou, ChinaDepartment of Clinical Laboratory, First Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Clinical Laboratory, First Medical Center of Chinese PLA General Hospital, Beijing, ChinaDepartment of Clinical Microbiology and Immunology, College of Pharmacy and Medical Laboratory, Army Medical University, Chongqing, ChinaDepartment of Basic Medical Laboratory, The 980th Hospital of the PLA Joint Logistical Support Force, Bethune International Peace Hospital, Shijiazhuang, ChinaDepartment of Clinical Laboratory, Medical School of Chinese PLA, Beijing, ChinaDepartment of Clinical Laboratory, First Medical Center of Chinese PLA General Hospital, Beijing, ChinaObjectiveAeromonas caviae (A. caviae) is one of the major etiological agents in human intestinal infections reported to be associated with a broad spectrum of extra-intestinal infections with increasing incidence over recent years. Although previous studies have established its significance as a causative agent of both bloodstream and gastrointestinal infections, the characteristics of A. caviae that cause extra-intestinal infections remain unilluminated.In this single-center retrospective study, we investigated epidemiological characteristics, antimicrobial resistance genes and phenotypes, virulence genes, and phyloevolution of 47 clinical A. caviae isolated from patients with extra-intestinal infections from 2017 to 2020.MethodsA. caviae strains were identified by biochemical tests and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS), ultimately confirmed to species level by whole-genome sequencing (WGS). Antimicrobial resistance and virulence genes were identified using the Comprehensive Antibiotic Resistance Database (CARD) and the virulence factor database (VFDB), respectively. Phylogenetic analysis of 47 clinical strains was performed by combining with 521 A. caviae strains from NCBI database.ResultsA. caviae was an opportunistic pathogen in immunocompromised patients, especially those with underlying hepatobiliary diseases and malignancies. 19 out of 47 isolates were identified as multidrug resistance (MDR) strains. Piperacillin-tazobactam, levofloxacin, gentamicin, amikacin with a resistance rate of less than 10% remained as options to treat extra-intestinal infections. 24 out of 47 isolates exhibited non-susceptibility to cephalosporins and cephamycins, all of which carried β-lactamase gene, including blaMOX, blaPER-3, blaOXA, blaNDM, and blaCphA. Most stains (98%, 46/47) carried at least one of the virulence genes, but extra-intestinal infections had a low mortality rate. Phylogenetic analysis indicated the risk of nosocomial transmission but revealed no outbreak. However, the emergence of MDR and β-lactamase resistance genes in extra-intestinal isolates of A. caviae is becoming an increasing risk to public health and requires attention.ConclusionsThis study strengthen our understanding of A.caviae isolated from extra-intestinal infections. It may contribute to the management of extra-intestinal infections as well as the prevention and control of drug resistance.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1084352/fullAeromonas caviaephylogenetic analysisantimicrobial resistanceextra-intestinal infectionsvirulence |
| spellingShingle | Yang Song Li-feng Wang Kuai Zhou Shuang Liu Ling Guo Li-yan Ye Jiang Gu Yan Cheng Ding-xia Shen Ding-xia Shen Epidemiological characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic analysis of Aeromonas caviae isolated from extra-intestinal infections Frontiers in Cellular and Infection Microbiology Aeromonas caviae phylogenetic analysis antimicrobial resistance extra-intestinal infections virulence |
| title | Epidemiological characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic analysis of Aeromonas caviae isolated from extra-intestinal infections |
| title_full | Epidemiological characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic analysis of Aeromonas caviae isolated from extra-intestinal infections |
| title_fullStr | Epidemiological characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic analysis of Aeromonas caviae isolated from extra-intestinal infections |
| title_full_unstemmed | Epidemiological characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic analysis of Aeromonas caviae isolated from extra-intestinal infections |
| title_short | Epidemiological characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic analysis of Aeromonas caviae isolated from extra-intestinal infections |
| title_sort | epidemiological characteristics virulence potential antimicrobial resistance profiles and phylogenetic analysis of aeromonas caviae isolated from extra intestinal infections |
| topic | Aeromonas caviae phylogenetic analysis antimicrobial resistance extra-intestinal infections virulence |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1084352/full |
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