Establishment of embryo transfer in the musk shrew (Suncus murinus)
The present study established techniques to induce pseudopregnancy, in vitro oocyte cultures from pronuclear to 2- to 4-cell stages, and embryo transfer in musk shrews, a reflex ovulator. Offspring were subsequently obtained by transferring in vivo-developed or in vitro-cultured embryos. Female musk...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2022-09-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/68/5/68_2022-003/_pdf/-char/en |
| _version_ | 1827767640625512448 |
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| author | Naoko INOUE Akinori HOTTA Teppei GOTO Masumi HIRABAYASHI Yoshihisa UENOYAMA Hiroko TSUKAMURA |
| author_facet | Naoko INOUE Akinori HOTTA Teppei GOTO Masumi HIRABAYASHI Yoshihisa UENOYAMA Hiroko TSUKAMURA |
| author_sort | Naoko INOUE |
| collection | DOAJ |
| description | The present study established techniques to induce pseudopregnancy, in vitro oocyte cultures from pronuclear to 2- to 4-cell stages, and embryo transfer in musk shrews, a reflex ovulator. Offspring were subsequently obtained by transferring in vivo-developed or in vitro-cultured embryos. Female musk shrews received human chronic gonadotropin (hCG), with or without mating stimuli, from vasectomized males to produce pseudopregnant recipients. Embryos at the 2- to 4-cell stage were collected 44–48 h after mating. Another set of embryos was collected 26–27 h after mating and then cultured for 20 h from the pronuclear to 2- to 4-cell stages. Subsequently, embryos were transferred into the oviducts of pseudopregnant recipients 24 or 48 h after the induction of pseudopregnancy. Offsprings were successfully obtained from recipients that received hCG 24 h before embryo transfer, regardless of mating stimuli. These techniques may be valuable for producing transgenic musk shrews. |
| first_indexed | 2024-03-11T12:01:51Z |
| format | Article |
| id | doaj.art-994d50ea4445472ba45f9eb2eb3a3c65 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T12:01:51Z |
| publishDate | 2022-09-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-994d50ea4445472ba45f9eb2eb3a3c652023-11-08T05:05:05ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002022-09-0168534034410.1262/jrd.2022-003jrdEstablishment of embryo transfer in the musk shrew (Suncus murinus)Naoko INOUE0Akinori HOTTA1Teppei GOTO2Masumi HIRABAYASHI3Yoshihisa UENOYAMA4Hiroko TSUKAMURA5Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, JapanGraduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, JapanCenter for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Aichi 444-8787, JapanCenter for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Aichi 444-8787, JapanGraduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, JapanGraduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, JapanThe present study established techniques to induce pseudopregnancy, in vitro oocyte cultures from pronuclear to 2- to 4-cell stages, and embryo transfer in musk shrews, a reflex ovulator. Offspring were subsequently obtained by transferring in vivo-developed or in vitro-cultured embryos. Female musk shrews received human chronic gonadotropin (hCG), with or without mating stimuli, from vasectomized males to produce pseudopregnant recipients. Embryos at the 2- to 4-cell stage were collected 44–48 h after mating. Another set of embryos was collected 26–27 h after mating and then cultured for 20 h from the pronuclear to 2- to 4-cell stages. Subsequently, embryos were transferred into the oviducts of pseudopregnant recipients 24 or 48 h after the induction of pseudopregnancy. Offsprings were successfully obtained from recipients that received hCG 24 h before embryo transfer, regardless of mating stimuli. These techniques may be valuable for producing transgenic musk shrews.https://www.jstage.jst.go.jp/article/jrd/68/5/68_2022-003/_pdf/-char/eninsectivorapeudopregnancyreflex ovulation |
| spellingShingle | Naoko INOUE Akinori HOTTA Teppei GOTO Masumi HIRABAYASHI Yoshihisa UENOYAMA Hiroko TSUKAMURA Establishment of embryo transfer in the musk shrew (Suncus murinus) The Journal of Reproduction and Development insectivora peudopregnancy reflex ovulation |
| title | Establishment of embryo transfer in the musk shrew (Suncus murinus) |
| title_full | Establishment of embryo transfer in the musk shrew (Suncus murinus) |
| title_fullStr | Establishment of embryo transfer in the musk shrew (Suncus murinus) |
| title_full_unstemmed | Establishment of embryo transfer in the musk shrew (Suncus murinus) |
| title_short | Establishment of embryo transfer in the musk shrew (Suncus murinus) |
| title_sort | establishment of embryo transfer in the musk shrew suncus murinus |
| topic | insectivora peudopregnancy reflex ovulation |
| url | https://www.jstage.jst.go.jp/article/jrd/68/5/68_2022-003/_pdf/-char/en |
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