An Integrated Centrifugal Degassed PDMS-Based Microfluidic Device for Serial Dilution
We propose an integrated serial dilution generator utilizing centrifugal force with a degassed polydimethylsiloxane (PDMS) microfluidic device. Using gas-soluble PDMS as a centrifugal microfluidic device material, the sample can be dragged in any arbitrary direction using vacuum-driven force, as opp...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/5/482 |
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| author | Anyang Wang Samaneh Moghadasi Boroujeni Philip J. Schneider Liam B. Christie Kyle A. Mancuso Stelios T. Andreadis Kwang W. Oh |
| author_facet | Anyang Wang Samaneh Moghadasi Boroujeni Philip J. Schneider Liam B. Christie Kyle A. Mancuso Stelios T. Andreadis Kwang W. Oh |
| author_sort | Anyang Wang |
| collection | DOAJ |
| description | We propose an integrated serial dilution generator utilizing centrifugal force with a degassed polydimethylsiloxane (PDMS) microfluidic device. Using gas-soluble PDMS as a centrifugal microfluidic device material, the sample can be dragged in any arbitrary direction using vacuum-driven force, as opposed to in a single direction, without adding further actuation components. The vacuum-driven force allows the device to avoid the formation of air bubbles and exhibit high tolerance in the surface condition. The device was then used for sample metering and sample transferring. In addition, centrifugal force was used for sample loading and sample mixing. In this study, a series of ten-fold serial dilutions ranging from <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mrow><mn>10</mn></mrow><mn>0</mn></msup></mrow></semantics></math></inline-formula> to <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mrow><mn>10</mn></mrow><mrow><mo>−</mo><mn>4</mn></mrow></msup></mrow></semantics></math></inline-formula> with about 8 μL in each chamber was achieved, while the serial dilution ratio and chamber volume could easily be altered by changing the geometrical designs of the device. As a proof of concept of our hybrid approach with the centrifugal and vacuum-driven forces, ten-fold serial dilutions of a cDNA (complementary DNA) sample were prepared using the device. Then, the diluted samples were collected by fine needles and subject to a quantitative polymerase chain reaction (qPCR), and the results were found to be in good agreement with those for samples prepared by manual pipetting. |
| first_indexed | 2024-03-10T12:03:01Z |
| format | Article |
| id | doaj.art-994e6e1d164a4d1487730c68c73ca3c1 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T12:03:01Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-994e6e1d164a4d1487730c68c73ca3c12023-11-21T16:49:28ZengMDPI AGMicromachines2072-666X2021-04-0112548210.3390/mi12050482An Integrated Centrifugal Degassed PDMS-Based Microfluidic Device for Serial DilutionAnyang Wang0Samaneh Moghadasi Boroujeni1Philip J. Schneider2Liam B. Christie3Kyle A. Mancuso4Stelios T. Andreadis5Kwang W. Oh6Sensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USADepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York (SUNY-Buffalo), Buffalo, NY 14260, USASensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USASensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USASensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USADepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York (SUNY-Buffalo), Buffalo, NY 14260, USASensors and MicroActuators Learning Lab (SMALL), Department of Electrical Engineering, The State University of New York at Buffalo, Buffalo, NY 14260, USAWe propose an integrated serial dilution generator utilizing centrifugal force with a degassed polydimethylsiloxane (PDMS) microfluidic device. Using gas-soluble PDMS as a centrifugal microfluidic device material, the sample can be dragged in any arbitrary direction using vacuum-driven force, as opposed to in a single direction, without adding further actuation components. The vacuum-driven force allows the device to avoid the formation of air bubbles and exhibit high tolerance in the surface condition. The device was then used for sample metering and sample transferring. In addition, centrifugal force was used for sample loading and sample mixing. In this study, a series of ten-fold serial dilutions ranging from <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mrow><mn>10</mn></mrow><mn>0</mn></msup></mrow></semantics></math></inline-formula> to <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mrow><mn>10</mn></mrow><mrow><mo>−</mo><mn>4</mn></mrow></msup></mrow></semantics></math></inline-formula> with about 8 μL in each chamber was achieved, while the serial dilution ratio and chamber volume could easily be altered by changing the geometrical designs of the device. As a proof of concept of our hybrid approach with the centrifugal and vacuum-driven forces, ten-fold serial dilutions of a cDNA (complementary DNA) sample were prepared using the device. Then, the diluted samples were collected by fine needles and subject to a quantitative polymerase chain reaction (qPCR), and the results were found to be in good agreement with those for samples prepared by manual pipetting.https://www.mdpi.com/2072-666X/12/5/482microfluidicvacuum pumpingcentrifugal pumpingserial dilutionqPCRPDMS |
| spellingShingle | Anyang Wang Samaneh Moghadasi Boroujeni Philip J. Schneider Liam B. Christie Kyle A. Mancuso Stelios T. Andreadis Kwang W. Oh An Integrated Centrifugal Degassed PDMS-Based Microfluidic Device for Serial Dilution Micromachines microfluidic vacuum pumping centrifugal pumping serial dilution qPCR PDMS |
| title | An Integrated Centrifugal Degassed PDMS-Based Microfluidic Device for Serial Dilution |
| title_full | An Integrated Centrifugal Degassed PDMS-Based Microfluidic Device for Serial Dilution |
| title_fullStr | An Integrated Centrifugal Degassed PDMS-Based Microfluidic Device for Serial Dilution |
| title_full_unstemmed | An Integrated Centrifugal Degassed PDMS-Based Microfluidic Device for Serial Dilution |
| title_short | An Integrated Centrifugal Degassed PDMS-Based Microfluidic Device for Serial Dilution |
| title_sort | integrated centrifugal degassed pdms based microfluidic device for serial dilution |
| topic | microfluidic vacuum pumping centrifugal pumping serial dilution qPCR PDMS |
| url | https://www.mdpi.com/2072-666X/12/5/482 |
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