IP-10 response to RD1 antigens might be a useful biomarker for monitoring tuberculosis therapy
<p>Abstract</p> <p>Background</p> <p>There is an urgent need of prognosis markers for tuberculosis (TB) to improve treatment strategies. The results of several studies show that the Interferon (IFN)-γ-specific response to the TB antigens of the QuantiFERON TB Gold (QFT-...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-05-01
|
| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/11/135 |
| _version_ | 1818208111982280704 |
|---|---|
| author | Girardi Enrico Ippolito Giuseppe Leportier Marc Thangaraj Satheesh Raman Balambal Raja Alamelu Kabeer Basirudeen Lagrange Philippe Goletti Delia |
| author_facet | Girardi Enrico Ippolito Giuseppe Leportier Marc Thangaraj Satheesh Raman Balambal Raja Alamelu Kabeer Basirudeen Lagrange Philippe Goletti Delia |
| author_sort | Girardi Enrico |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>There is an urgent need of prognosis markers for tuberculosis (TB) to improve treatment strategies. The results of several studies show that the Interferon (IFN)-γ-specific response to the TB antigens of the QuantiFERON TB Gold (QFT-IT antigens) decreases after successful TB therapy. The objective of this study was to evaluate whether there are factors other than IFN-γ [such as IFN-γ inducible protein (IP)-10 which has also been associated with TB] in response to QFT-IT antigens that can be used as biomarkers for monitoring TB treatment.</p> <p>Methods</p> <p>In this exploratory study we assessed the changes in IP-10 secretion in response to QFT-IT antigens and RD1 peptides selected by computational analysis in 17 patients with active TB at the time of diagnosis and after 6 months of treatment. The IFN-γ response to QFT-IT antigens and RD1 selected peptides was evaluated as a control. A non-parametric Wilcoxon signed-rank test for paired comparisons was used to compare the continuous variables at the time of diagnosis and at therapy completion. A Chi-square test was used to compare proportions.</p> <p>Results</p> <p>We did not observe significant IP-10 changes in whole blood from either NIL or QFT-IT antigen tubes, after 1-day stimulation, between baseline and therapy completion (p = 0.08 and p = 0.7 respectively). Conversely, the level of IP-10 release to RD1 selected peptides was significantly different (p = 0.006). Similar results were obtained when we detected the IFN-γ in response to the QFT-IT antigens (p = 0.06) and RD1 selected peptides (p = 0.0003). The proportion of the IP-10 responders to the QFT-IT antigens did not significantly change between baseline and therapy completion (p = 0.6), whereas it significantly changed in response to RD1 selected peptides (p = 0.002). The proportion of IFN-γ responders between baseline and therapy completion was not significant for QFT-IT antigens (p = 0.2), whereas it was significant for the RD1 selected peptides (p = 0.002), confirming previous observations.</p> <p>Conclusions</p> <p>Our preliminary study provides an interesting hypothesis: IP-10 response to RD1 selected peptides (similar to IFN-γ) might be a useful biomarker for monitoring therapy efficacy in patients with active TB. However, further studies in larger cohorts are needed to confirm the consistency of these study results.</p> |
| first_indexed | 2024-12-12T04:39:38Z |
| format | Article |
| id | doaj.art-994eb48feb4b43ddb0cd1be6afeaf747 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-12T04:39:38Z |
| publishDate | 2011-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-994eb48feb4b43ddb0cd1be6afeaf7472022-12-22T00:37:51ZengBMCBMC Infectious Diseases1471-23342011-05-0111113510.1186/1471-2334-11-135IP-10 response to RD1 antigens might be a useful biomarker for monitoring tuberculosis therapyGirardi EnricoIppolito GiuseppeLeportier MarcThangaraj SatheeshRaman BalambalRaja AlameluKabeer BasirudeenLagrange PhilippeGoletti Delia<p>Abstract</p> <p>Background</p> <p>There is an urgent need of prognosis markers for tuberculosis (TB) to improve treatment strategies. The results of several studies show that the Interferon (IFN)-γ-specific response to the TB antigens of the QuantiFERON TB Gold (QFT-IT antigens) decreases after successful TB therapy. The objective of this study was to evaluate whether there are factors other than IFN-γ [such as IFN-γ inducible protein (IP)-10 which has also been associated with TB] in response to QFT-IT antigens that can be used as biomarkers for monitoring TB treatment.</p> <p>Methods</p> <p>In this exploratory study we assessed the changes in IP-10 secretion in response to QFT-IT antigens and RD1 peptides selected by computational analysis in 17 patients with active TB at the time of diagnosis and after 6 months of treatment. The IFN-γ response to QFT-IT antigens and RD1 selected peptides was evaluated as a control. A non-parametric Wilcoxon signed-rank test for paired comparisons was used to compare the continuous variables at the time of diagnosis and at therapy completion. A Chi-square test was used to compare proportions.</p> <p>Results</p> <p>We did not observe significant IP-10 changes in whole blood from either NIL or QFT-IT antigen tubes, after 1-day stimulation, between baseline and therapy completion (p = 0.08 and p = 0.7 respectively). Conversely, the level of IP-10 release to RD1 selected peptides was significantly different (p = 0.006). Similar results were obtained when we detected the IFN-γ in response to the QFT-IT antigens (p = 0.06) and RD1 selected peptides (p = 0.0003). The proportion of the IP-10 responders to the QFT-IT antigens did not significantly change between baseline and therapy completion (p = 0.6), whereas it significantly changed in response to RD1 selected peptides (p = 0.002). The proportion of IFN-γ responders between baseline and therapy completion was not significant for QFT-IT antigens (p = 0.2), whereas it was significant for the RD1 selected peptides (p = 0.002), confirming previous observations.</p> <p>Conclusions</p> <p>Our preliminary study provides an interesting hypothesis: IP-10 response to RD1 selected peptides (similar to IFN-γ) might be a useful biomarker for monitoring therapy efficacy in patients with active TB. However, further studies in larger cohorts are needed to confirm the consistency of these study results.</p>http://www.biomedcentral.com/1471-2334/11/135 |
| spellingShingle | Girardi Enrico Ippolito Giuseppe Leportier Marc Thangaraj Satheesh Raman Balambal Raja Alamelu Kabeer Basirudeen Lagrange Philippe Goletti Delia IP-10 response to RD1 antigens might be a useful biomarker for monitoring tuberculosis therapy BMC Infectious Diseases |
| title | IP-10 response to RD1 antigens might be a useful biomarker for monitoring tuberculosis therapy |
| title_full | IP-10 response to RD1 antigens might be a useful biomarker for monitoring tuberculosis therapy |
| title_fullStr | IP-10 response to RD1 antigens might be a useful biomarker for monitoring tuberculosis therapy |
| title_full_unstemmed | IP-10 response to RD1 antigens might be a useful biomarker for monitoring tuberculosis therapy |
| title_short | IP-10 response to RD1 antigens might be a useful biomarker for monitoring tuberculosis therapy |
| title_sort | ip 10 response to rd1 antigens might be a useful biomarker for monitoring tuberculosis therapy |
| url | http://www.biomedcentral.com/1471-2334/11/135 |
| work_keys_str_mv | AT girardienrico ip10responsetord1antigensmightbeausefulbiomarkerformonitoringtuberculosistherapy AT ippolitogiuseppe ip10responsetord1antigensmightbeausefulbiomarkerformonitoringtuberculosistherapy AT leportiermarc ip10responsetord1antigensmightbeausefulbiomarkerformonitoringtuberculosistherapy AT thangarajsatheesh ip10responsetord1antigensmightbeausefulbiomarkerformonitoringtuberculosistherapy AT ramanbalambal ip10responsetord1antigensmightbeausefulbiomarkerformonitoringtuberculosistherapy AT rajaalamelu ip10responsetord1antigensmightbeausefulbiomarkerformonitoringtuberculosistherapy AT kabeerbasirudeen ip10responsetord1antigensmightbeausefulbiomarkerformonitoringtuberculosistherapy AT lagrangephilippe ip10responsetord1antigensmightbeausefulbiomarkerformonitoringtuberculosistherapy AT golettidelia ip10responsetord1antigensmightbeausefulbiomarkerformonitoringtuberculosistherapy |