Histochemistry and infrared microspectroscopy of lignified tissue in young stems of Struthanthus vulgaris Mart.
In this study, we aimed to determine lignified tissue in young stems of Struthanthus vulgaris Mart. by infrared microspectroscopy and histochemical methods as well as by fluorescence microscopy. Struthanthus vulgaris Mart. is a mistletoe species that belongs to the Loranthaceae family. A brief anato...
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| Format: | Article |
| Language: | English |
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Universidade Federal Rural do Rio de Janeiro
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| Series: | Floresta e Ambiente |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872013000200014&lng=en&tlng=en |
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| author | Gisely de Lima Oliveira Heber dos Santos Abreu Tatiana de Felice Elias Bruno Couto da Silva Monique de Moura Gurgel Carlos Henrique Rocha Gonçalves Geraldo Luiz Gonçalves Soares |
| author_facet | Gisely de Lima Oliveira Heber dos Santos Abreu Tatiana de Felice Elias Bruno Couto da Silva Monique de Moura Gurgel Carlos Henrique Rocha Gonçalves Geraldo Luiz Gonçalves Soares |
| author_sort | Gisely de Lima Oliveira |
| collection | DOAJ |
| description | In this study, we aimed to determine lignified tissue in young stems of Struthanthus vulgaris Mart. by infrared microspectroscopy and histochemical methods as well as by fluorescence microscopy. Struthanthus vulgaris Mart. is a mistletoe species that belongs to the Loranthaceae family. A brief anatomical description was also carried out. The first procedure for analysis was to elaborate anatomical cross sections (20-30 µm) from young stems before and after treatment with NaOH 1%. This procedure was applied to release possible low molecular mass phenolic compounds. Safranin-astra blue was used to distinguish anatomical tissues while Wiesner test enabled verification of lignified pericyclic fibers. Infrared microspectroscopy analysis confirmed the presence of lignin in this region according to the following spectral signals: 1600 (shoulder), 1511, 1453, 1338 and 1244 cm-1. Analyses of the cross section of young stems under fluorescence microscopy before and after treatment with NaOH 1% allowed us to confirm the presence of low mass phenolic compounds in the region of pericyclic fibers. |
| first_indexed | 2024-04-12T09:40:06Z |
| format | Article |
| id | doaj.art-9951855f236b4a87a907677ba3f2d03c |
| institution | Directory Open Access Journal |
| issn | 2179-8087 |
| language | English |
| last_indexed | 2024-04-12T09:40:06Z |
| publisher | Universidade Federal Rural do Rio de Janeiro |
| record_format | Article |
| series | Floresta e Ambiente |
| spelling | doaj.art-9951855f236b4a87a907677ba3f2d03c2022-12-22T03:38:06ZengUniversidade Federal Rural do Rio de JaneiroFloresta e Ambiente2179-808720225726310.4322/floram.2013.016S2179-80872013000200014Histochemistry and infrared microspectroscopy of lignified tissue in young stems of Struthanthus vulgaris Mart.Gisely de Lima Oliveira0Heber dos Santos Abreu1Tatiana de Felice Elias2Bruno Couto da Silva3Monique de Moura Gurgel4Carlos Henrique Rocha Gonçalves5Geraldo Luiz Gonçalves Soares6Universidade Federal Rural do Rio de JaneiroUniversidade Federal Rural do Rio de JaneiroUniversidade Federal Rural do Rio de JaneiroUniversidade Federal Rural do Rio de JaneiroUniversidade Federal Rural do Rio de JaneiroUniversidade Federal Rural do Rio de JaneiroUniversidade Federal do Rio Grande do SulIn this study, we aimed to determine lignified tissue in young stems of Struthanthus vulgaris Mart. by infrared microspectroscopy and histochemical methods as well as by fluorescence microscopy. Struthanthus vulgaris Mart. is a mistletoe species that belongs to the Loranthaceae family. A brief anatomical description was also carried out. The first procedure for analysis was to elaborate anatomical cross sections (20-30 µm) from young stems before and after treatment with NaOH 1%. This procedure was applied to release possible low molecular mass phenolic compounds. Safranin-astra blue was used to distinguish anatomical tissues while Wiesner test enabled verification of lignified pericyclic fibers. Infrared microspectroscopy analysis confirmed the presence of lignin in this region according to the following spectral signals: 1600 (shoulder), 1511, 1453, 1338 and 1244 cm-1. Analyses of the cross section of young stems under fluorescence microscopy before and after treatment with NaOH 1% allowed us to confirm the presence of low mass phenolic compounds in the region of pericyclic fibers.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872013000200014&lng=en&tlng=enmistletoe speciesfiberslignifications |
| spellingShingle | Gisely de Lima Oliveira Heber dos Santos Abreu Tatiana de Felice Elias Bruno Couto da Silva Monique de Moura Gurgel Carlos Henrique Rocha Gonçalves Geraldo Luiz Gonçalves Soares Histochemistry and infrared microspectroscopy of lignified tissue in young stems of Struthanthus vulgaris Mart. Floresta e Ambiente mistletoe species fibers lignifications |
| title | Histochemistry and infrared microspectroscopy of lignified tissue in young stems of Struthanthus vulgaris Mart. |
| title_full | Histochemistry and infrared microspectroscopy of lignified tissue in young stems of Struthanthus vulgaris Mart. |
| title_fullStr | Histochemistry and infrared microspectroscopy of lignified tissue in young stems of Struthanthus vulgaris Mart. |
| title_full_unstemmed | Histochemistry and infrared microspectroscopy of lignified tissue in young stems of Struthanthus vulgaris Mart. |
| title_short | Histochemistry and infrared microspectroscopy of lignified tissue in young stems of Struthanthus vulgaris Mart. |
| title_sort | histochemistry and infrared microspectroscopy of lignified tissue in young stems of struthanthus vulgaris mart |
| topic | mistletoe species fibers lignifications |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872013000200014&lng=en&tlng=en |
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