Synthesis of Yellow-Fluorescent Carbon Nano-dots by Microplasma for Imaging and Photocatalytic Inactivation of Cancer Cells
Abstract In recent years, multifunctional nanoparticles with combined diagnostic and therapeutic functions show great promise in nanomedicine. In this study, we report the environmentally friendly synthesis of fluorescent carbon nano-dots such as carbon quantum dots (CQDs) by microplasma using o-phe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-01-01
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| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s11671-021-03478-2 |
| _version_ | 1797760572122464256 |
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| author | Xing Qin Jinlin Liu Qing Zhang Wantao Chen Xiaoxia Zhong Jie He |
| author_facet | Xing Qin Jinlin Liu Qing Zhang Wantao Chen Xiaoxia Zhong Jie He |
| author_sort | Xing Qin |
| collection | DOAJ |
| description | Abstract In recent years, multifunctional nanoparticles with combined diagnostic and therapeutic functions show great promise in nanomedicine. In this study, we report the environmentally friendly synthesis of fluorescent carbon nano-dots such as carbon quantum dots (CQDs) by microplasma using o-phenylenediamine. The produced CQDs exhibited a wide absorption peaks at 380–500 nm and emitted bright yellow fluorescence with a peak at 550 nm. The CQDs were rapidly taken up by HeLa cancer cells. When excited under blue light, a bright yellow fluorescence signal and intense reactive oxygen species (ROS) were efficiently produced, enabling simultaneous fluorescent cancer cell imaging and photodynamic inactivation, with a 40% decrease in relative cell viability. Furthermore, about 98% cells were active after the incubation with 400 μg mL−1 CQDs in the dark, which revealed the excellent biocompatibility of CQDs. Hence, the newly prepared CQDs are thus demonstrated to be materials which might be effective and safe to use for in vivo bioimaging and imaging-guided cancer therapy. |
| first_indexed | 2024-03-12T19:00:31Z |
| format | Article |
| id | doaj.art-99534a204c374288bcd1469f633844d8 |
| institution | Directory Open Access Journal |
| issn | 1556-276X |
| language | English |
| last_indexed | 2024-03-12T19:00:31Z |
| publishDate | 2021-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-99534a204c374288bcd1469f633844d82023-08-02T06:40:57ZengSpringerOpenNanoscale Research Letters1556-276X2021-01-011611910.1186/s11671-021-03478-2Synthesis of Yellow-Fluorescent Carbon Nano-dots by Microplasma for Imaging and Photocatalytic Inactivation of Cancer CellsXing Qin0Jinlin Liu1Qing Zhang2Wantao Chen3Xiaoxia Zhong4Jie He5Department of Oral and Maxillofacial-Head and Neck Surgery, Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineHunan Key Laboratory of Oral Health Research and Hunan 3D Printing Engineering Research Center of Oral Care and Hunan Clinical Research Center of Oral Major Diseases and Oral Health and Xiangya Stomatological Hospital and Xiangya School of Stomatology, Central South UniversityState Key Laboratory of Advanced Optical Communication Systems and Networks, Key Laboratory for Laser Plasmas (Ministry of Education), School of Physics and Astronomy, Shanghai Jiao Tong UniversityDepartment of Oral and Maxillofacial-Head and Neck Surgery, Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineState Key Laboratory of Advanced Optical Communication Systems and Networks, Key Laboratory for Laser Plasmas (Ministry of Education), School of Physics and Astronomy, Shanghai Jiao Tong UniversityDepartment of Oral and Maxillofacial-Head and Neck Surgery, Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineAbstract In recent years, multifunctional nanoparticles with combined diagnostic and therapeutic functions show great promise in nanomedicine. In this study, we report the environmentally friendly synthesis of fluorescent carbon nano-dots such as carbon quantum dots (CQDs) by microplasma using o-phenylenediamine. The produced CQDs exhibited a wide absorption peaks at 380–500 nm and emitted bright yellow fluorescence with a peak at 550 nm. The CQDs were rapidly taken up by HeLa cancer cells. When excited under blue light, a bright yellow fluorescence signal and intense reactive oxygen species (ROS) were efficiently produced, enabling simultaneous fluorescent cancer cell imaging and photodynamic inactivation, with a 40% decrease in relative cell viability. Furthermore, about 98% cells were active after the incubation with 400 μg mL−1 CQDs in the dark, which revealed the excellent biocompatibility of CQDs. Hence, the newly prepared CQDs are thus demonstrated to be materials which might be effective and safe to use for in vivo bioimaging and imaging-guided cancer therapy.https://doi.org/10.1186/s11671-021-03478-2Atmospheric pressure microplasmaBioimagingCarbon quantum dotsPhotodynamic therapyYellow emission |
| spellingShingle | Xing Qin Jinlin Liu Qing Zhang Wantao Chen Xiaoxia Zhong Jie He Synthesis of Yellow-Fluorescent Carbon Nano-dots by Microplasma for Imaging and Photocatalytic Inactivation of Cancer Cells Nanoscale Research Letters Atmospheric pressure microplasma Bioimaging Carbon quantum dots Photodynamic therapy Yellow emission |
| title | Synthesis of Yellow-Fluorescent Carbon Nano-dots by Microplasma for Imaging and Photocatalytic Inactivation of Cancer Cells |
| title_full | Synthesis of Yellow-Fluorescent Carbon Nano-dots by Microplasma for Imaging and Photocatalytic Inactivation of Cancer Cells |
| title_fullStr | Synthesis of Yellow-Fluorescent Carbon Nano-dots by Microplasma for Imaging and Photocatalytic Inactivation of Cancer Cells |
| title_full_unstemmed | Synthesis of Yellow-Fluorescent Carbon Nano-dots by Microplasma for Imaging and Photocatalytic Inactivation of Cancer Cells |
| title_short | Synthesis of Yellow-Fluorescent Carbon Nano-dots by Microplasma for Imaging and Photocatalytic Inactivation of Cancer Cells |
| title_sort | synthesis of yellow fluorescent carbon nano dots by microplasma for imaging and photocatalytic inactivation of cancer cells |
| topic | Atmospheric pressure microplasma Bioimaging Carbon quantum dots Photodynamic therapy Yellow emission |
| url | https://doi.org/10.1186/s11671-021-03478-2 |
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