The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats
Abstract When orthodontic forces are applied to teeth, bone remodeling, which consists of bone resorption and bone formation, occurs around the teeth. Transient receptor potential vanilloid 2 (TRPV2) is a cation channel expressed in various cell types that responds to various stimuli, including mech...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-08-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-41019-2 |
| _version_ | 1797453225458139136 |
|---|---|
| author | Shohei Shigemi Tadasu Sato Mayuri Sakamoto Takehiro Yajima Takahiro Honda Hiroka Tsumaki Toru Deguchi Hiroyuki Ichikawa Tomohiro Fukunaga Itaru Mizoguchi |
| author_facet | Shohei Shigemi Tadasu Sato Mayuri Sakamoto Takehiro Yajima Takahiro Honda Hiroka Tsumaki Toru Deguchi Hiroyuki Ichikawa Tomohiro Fukunaga Itaru Mizoguchi |
| author_sort | Shohei Shigemi |
| collection | DOAJ |
| description | Abstract When orthodontic forces are applied to teeth, bone remodeling, which consists of bone resorption and bone formation, occurs around the teeth. Transient receptor potential vanilloid 2 (TRPV2) is a cation channel expressed in various cell types that responds to various stimuli, including mechanical stress, and involved in calcium oscillations during the early stages of osteoclast differentiation. However, in vivo expression of TRPV2 in osteoclasts has not yet been reported, and temporo-spatial expression of TRPV2 during osteoclast differentiation is unclear. In this study, we examined the TRPV2 expression during experimental tooth movement and assessed the effect of TRPV2 on osteoclast differentiation. TRPV2 was detected on day 1 after experimental tooth movement on the compression side, and the number of TRPV2-expressing cells significantly increased on day 7. These TRPV2-expressing cells had a single, or multiple nuclei and were positive for TRAP activity. Consistent with these in vivo findings, in vitro experiments using RAW264.7 osteoclast progenitor cells showed that TRPV2 mRNA was increased at the early stage of osteoclast differentiation and maintained until the late stage. Furthermore, a TRPV2 channel selective antagonist significantly inhibited osteoclast differentiation. These findings suggest that TRPV2 may have a regulatory role in osteoclast differentiation during orthodontic tooth movement. |
| first_indexed | 2024-03-09T15:19:43Z |
| format | Article |
| id | doaj.art-995393689b9448ed8811fa939ec4fa58 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:19:43Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-995393689b9448ed8811fa939ec4fa582023-11-26T12:54:31ZengNature PortfolioScientific Reports2045-23222023-08-0113111310.1038/s41598-023-41019-2The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in ratsShohei Shigemi0Tadasu Sato1Mayuri Sakamoto2Takehiro Yajima3Takahiro Honda4Hiroka Tsumaki5Toru Deguchi6Hiroyuki Ichikawa7Tomohiro Fukunaga8Itaru Mizoguchi9Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of DentistryDivision of Oral and Craniofacial Anatomy, Tohoku University Graduate School of DentistryDivision of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of DentistryDivision of Oral and Craniofacial Anatomy, Tohoku University Graduate School of DentistryDivision of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of DentistryDivision of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of DentistryDivision of Orthodontics and Prosthodontics, University of LouisvilleDivision of Oral and Craniofacial Anatomy, Tohoku University Graduate School of DentistryDivision of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of DentistryDivision of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of DentistryAbstract When orthodontic forces are applied to teeth, bone remodeling, which consists of bone resorption and bone formation, occurs around the teeth. Transient receptor potential vanilloid 2 (TRPV2) is a cation channel expressed in various cell types that responds to various stimuli, including mechanical stress, and involved in calcium oscillations during the early stages of osteoclast differentiation. However, in vivo expression of TRPV2 in osteoclasts has not yet been reported, and temporo-spatial expression of TRPV2 during osteoclast differentiation is unclear. In this study, we examined the TRPV2 expression during experimental tooth movement and assessed the effect of TRPV2 on osteoclast differentiation. TRPV2 was detected on day 1 after experimental tooth movement on the compression side, and the number of TRPV2-expressing cells significantly increased on day 7. These TRPV2-expressing cells had a single, or multiple nuclei and were positive for TRAP activity. Consistent with these in vivo findings, in vitro experiments using RAW264.7 osteoclast progenitor cells showed that TRPV2 mRNA was increased at the early stage of osteoclast differentiation and maintained until the late stage. Furthermore, a TRPV2 channel selective antagonist significantly inhibited osteoclast differentiation. These findings suggest that TRPV2 may have a regulatory role in osteoclast differentiation during orthodontic tooth movement.https://doi.org/10.1038/s41598-023-41019-2 |
| spellingShingle | Shohei Shigemi Tadasu Sato Mayuri Sakamoto Takehiro Yajima Takahiro Honda Hiroka Tsumaki Toru Deguchi Hiroyuki Ichikawa Tomohiro Fukunaga Itaru Mizoguchi The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats Scientific Reports |
| title | The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
| title_full | The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
| title_fullStr | The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
| title_full_unstemmed | The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
| title_short | The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
| title_sort | role of trpv2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats |
| url | https://doi.org/10.1038/s41598-023-41019-2 |
| work_keys_str_mv | AT shoheishigemi theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT tadasusato theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT mayurisakamoto theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT takehiroyajima theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT takahirohonda theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT hirokatsumaki theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT torudeguchi theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT hiroyukiichikawa theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT tomohirofukunaga theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT itarumizoguchi theroleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT shoheishigemi roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT tadasusato roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT mayurisakamoto roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT takehiroyajima roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT takahirohonda roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT hirokatsumaki roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT torudeguchi roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT hiroyukiichikawa roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT tomohirofukunaga roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats AT itarumizoguchi roleoftrpv2asaregulatorontheosteoclastdifferentiationduringorthodontictoothmovementinrats |