Genome-Wide Association Analysis Identifies Resistance Loci for Bacterial Leaf Streak Resistance in Rice (<i>Oryza sativa</i> L.)
Bacterial leaf streak (BLS) caused by <i>Xanthomonas oryzae</i> pv. <i>oryzicola</i> (<i>Xoc</i>) is one of the most devastating diseases in rice production areas, especially in humid tropical and subtropical zones throughout Asia and worldwide. A genome-wide asso...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/12/1673 |
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| author | Wannapa Sattayachiti Samart Wanchana Siwaret Arikit Phakchana Nubankoh Sujin Patarapuwadol Apichart Vanavichit Clive T. Darwell Theerayut Toojinda |
| author_facet | Wannapa Sattayachiti Samart Wanchana Siwaret Arikit Phakchana Nubankoh Sujin Patarapuwadol Apichart Vanavichit Clive T. Darwell Theerayut Toojinda |
| author_sort | Wannapa Sattayachiti |
| collection | DOAJ |
| description | Bacterial leaf streak (BLS) caused by <i>Xanthomonas oryzae</i> pv. <i>oryzicola</i> (<i>Xoc</i>) is one of the most devastating diseases in rice production areas, especially in humid tropical and subtropical zones throughout Asia and worldwide. A genome-wide association study (GWAS) analysis conducted on a collection of 236 diverse rice accessions, mainly <i>indica</i> varieties, identified 12 quantitative trait loci (QTLs) on chromosomes 1, 2, 3, 4, 5, 8, 9 and 11, conferring resistance to five representative isolates of Thai <i>Xoc</i>. Of these, five QTLs conferred resistance to more than one <i>Xoc</i> isolates. Two QTLs, <i>qBLS5.1</i> and <i>qBLS2.3,</i> were considered promising QTLs for broad-spectrum resistance to BLS. The <i>xa5</i> gene was proposed as a potential candidate gene for <i>qBLS5.1</i> and three genes, encoding pectinesterase inhibitor <i>(OsPEI)</i>, eukaryotic zinc-binding protein (<i>OsRAR1)</i>, and NDP epimerase function, were proposed as candidate genes for <i>qBLS2.3</i>. Results from this study provide an insight into the potential QTLs and candidate genes for BLS resistance in rice. The recessive <i>xa5</i> gene is suggested as a potential candidate for strong influence on broad-spectrum resistance and as a focal target in rice breeding programs for BLS resistance. |
| first_indexed | 2024-03-10T14:27:20Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T14:27:20Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-9954fb41c8e341c59d8f1904908468622023-11-20T22:51:54ZengMDPI AGPlants2223-77472020-11-01912167310.3390/plants9121673Genome-Wide Association Analysis Identifies Resistance Loci for Bacterial Leaf Streak Resistance in Rice (<i>Oryza sativa</i> L.)Wannapa Sattayachiti0Samart Wanchana1Siwaret Arikit2Phakchana Nubankoh3Sujin Patarapuwadol4Apichart Vanavichit5Clive T. Darwell6Theerayut Toojinda7Plant Breeding Program, Faculty of Agriculture at Kamphaeng Saen, Kesetsart University, Nakhon Pathom 73140, ThailandNational Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Pahonyothin Road, Khlong Nueng, Khlong Luang, PathumThani 12120, ThailandDepartment of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, ThailandNational Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Pahonyothin Road, Khlong Nueng, Khlong Luang, PathumThani 12120, ThailandDepartment of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, ThailandDepartment of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, ThailandNational Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Pahonyothin Road, Khlong Nueng, Khlong Luang, PathumThani 12120, ThailandNational Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Pahonyothin Road, Khlong Nueng, Khlong Luang, PathumThani 12120, ThailandBacterial leaf streak (BLS) caused by <i>Xanthomonas oryzae</i> pv. <i>oryzicola</i> (<i>Xoc</i>) is one of the most devastating diseases in rice production areas, especially in humid tropical and subtropical zones throughout Asia and worldwide. A genome-wide association study (GWAS) analysis conducted on a collection of 236 diverse rice accessions, mainly <i>indica</i> varieties, identified 12 quantitative trait loci (QTLs) on chromosomes 1, 2, 3, 4, 5, 8, 9 and 11, conferring resistance to five representative isolates of Thai <i>Xoc</i>. Of these, five QTLs conferred resistance to more than one <i>Xoc</i> isolates. Two QTLs, <i>qBLS5.1</i> and <i>qBLS2.3,</i> were considered promising QTLs for broad-spectrum resistance to BLS. The <i>xa5</i> gene was proposed as a potential candidate gene for <i>qBLS5.1</i> and three genes, encoding pectinesterase inhibitor <i>(OsPEI)</i>, eukaryotic zinc-binding protein (<i>OsRAR1)</i>, and NDP epimerase function, were proposed as candidate genes for <i>qBLS2.3</i>. Results from this study provide an insight into the potential QTLs and candidate genes for BLS resistance in rice. The recessive <i>xa5</i> gene is suggested as a potential candidate for strong influence on broad-spectrum resistance and as a focal target in rice breeding programs for BLS resistance.https://www.mdpi.com/2223-7747/9/12/1673bacterial leaf streak disease<i>Xanthomonas oryzae</i> pv. <i>oryzicola</i> (<i>Xoc</i>)ricegenome-wide association (GWAS)broad-spectrum resistance |
| spellingShingle | Wannapa Sattayachiti Samart Wanchana Siwaret Arikit Phakchana Nubankoh Sujin Patarapuwadol Apichart Vanavichit Clive T. Darwell Theerayut Toojinda Genome-Wide Association Analysis Identifies Resistance Loci for Bacterial Leaf Streak Resistance in Rice (<i>Oryza sativa</i> L.) Plants bacterial leaf streak disease <i>Xanthomonas oryzae</i> pv. <i>oryzicola</i> (<i>Xoc</i>) rice genome-wide association (GWAS) broad-spectrum resistance |
| title | Genome-Wide Association Analysis Identifies Resistance Loci for Bacterial Leaf Streak Resistance in Rice (<i>Oryza sativa</i> L.) |
| title_full | Genome-Wide Association Analysis Identifies Resistance Loci for Bacterial Leaf Streak Resistance in Rice (<i>Oryza sativa</i> L.) |
| title_fullStr | Genome-Wide Association Analysis Identifies Resistance Loci for Bacterial Leaf Streak Resistance in Rice (<i>Oryza sativa</i> L.) |
| title_full_unstemmed | Genome-Wide Association Analysis Identifies Resistance Loci for Bacterial Leaf Streak Resistance in Rice (<i>Oryza sativa</i> L.) |
| title_short | Genome-Wide Association Analysis Identifies Resistance Loci for Bacterial Leaf Streak Resistance in Rice (<i>Oryza sativa</i> L.) |
| title_sort | genome wide association analysis identifies resistance loci for bacterial leaf streak resistance in rice i oryza sativa i l |
| topic | bacterial leaf streak disease <i>Xanthomonas oryzae</i> pv. <i>oryzicola</i> (<i>Xoc</i>) rice genome-wide association (GWAS) broad-spectrum resistance |
| url | https://www.mdpi.com/2223-7747/9/12/1673 |
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