Glucose-6-phosphate dehydrogenase activity measured by spectrophotometry and associated genetic variants from the Oromiya zone, Ethiopia
Abstract Background The study aimed to gain first data on the prevalence of G6PD enzyme deficiency measured by spectrophotometry and associated genetic variants in Jimma and surroundings, Ethiopia. The area is a Plasmodium vivax endemic region, but 8-aminoquinolines such as primaquine are not recomm...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-10-01
|
| Series: | Malaria Journal |
| Online Access: | http://link.springer.com/article/10.1186/s12936-018-2510-3 |
| _version_ | 1828407821133152256 |
|---|---|
| author | Nora Kießling Joaquin Brintrup Ahmed Zeynudin Nuredin Abduselam Sylvia Götz Margith Mack Michael Pritsch Andreas Wieser Elisabeth Kohne Nicole Berens-Riha |
| author_facet | Nora Kießling Joaquin Brintrup Ahmed Zeynudin Nuredin Abduselam Sylvia Götz Margith Mack Michael Pritsch Andreas Wieser Elisabeth Kohne Nicole Berens-Riha |
| author_sort | Nora Kießling |
| collection | DOAJ |
| description | Abstract Background The study aimed to gain first data on the prevalence of G6PD enzyme deficiency measured by spectrophotometry and associated genetic variants in Jimma and surroundings, Ethiopia. The area is a Plasmodium vivax endemic region, but 8-aminoquinolines such as primaquine are not recommended as G6PD testing is not available. Methods Healthy volunteers were recruited at Jimma University, Ethiopia. Enzyme activity was tested by spectrophotometry at the University of Ulm, Germany. A G6PD RDT (Binax NOW® G6PD, Alere, USA) was additionally performed. The G6PD gene was analysed for polymorphisms in a sub-population. Tests for haemoglobinopathies and the presence of malaria parasites were conducted. Results No severe or moderate (cut-off 60%) G6PD deficiency was found in 206 volunteers. Median male activity was 6.1 U/g Hb. Eleven participants (5.4%) showed activities between 70 and 80%. No haemoglobinopathy was detected. None of the subjects showed asymptomatic parasitaemia. One G6PD-A+ variant (A376G) and one new non-synonymous mutation (G445A) were found. Conclusions As the prevalence of G6PD deficiency seems low in this area, the use of 8-aminoquinolines should be encouraged. However, reliable G6PD testing methods have to be implemented and safe cut-off levels need to be defined. |
| first_indexed | 2024-12-10T11:30:06Z |
| format | Article |
| id | doaj.art-995904eeabe94787a0e79a957cc55437 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-10T11:30:06Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-995904eeabe94787a0e79a957cc554372022-12-22T01:50:37ZengBMCMalaria Journal1475-28752018-10-0117111010.1186/s12936-018-2510-3Glucose-6-phosphate dehydrogenase activity measured by spectrophotometry and associated genetic variants from the Oromiya zone, EthiopiaNora Kießling0Joaquin Brintrup1Ahmed Zeynudin2Nuredin Abduselam3Sylvia Götz4Margith Mack5Michael Pritsch6Andreas Wieser7Elisabeth Kohne8Nicole Berens-Riha9Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU)Hemoglobin Laboratory, Department of Pediatrics, University Hospital UlmDepartment of Medical Laboratory Sciences, Jimma UniversityDepartment of Medical Laboratory Sciences, Jimma UniversityHemoglobin Laboratory, Department of Pediatrics, University Hospital UlmHemoglobin Laboratory, Department of Pediatrics, University Hospital UlmDivision of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU)Division of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU)Hemoglobin Laboratory, Department of Pediatrics, University Hospital UlmDivision of Infectious Diseases and Tropical Medicine, Medical Center of the University of Munich (LMU)Abstract Background The study aimed to gain first data on the prevalence of G6PD enzyme deficiency measured by spectrophotometry and associated genetic variants in Jimma and surroundings, Ethiopia. The area is a Plasmodium vivax endemic region, but 8-aminoquinolines such as primaquine are not recommended as G6PD testing is not available. Methods Healthy volunteers were recruited at Jimma University, Ethiopia. Enzyme activity was tested by spectrophotometry at the University of Ulm, Germany. A G6PD RDT (Binax NOW® G6PD, Alere, USA) was additionally performed. The G6PD gene was analysed for polymorphisms in a sub-population. Tests for haemoglobinopathies and the presence of malaria parasites were conducted. Results No severe or moderate (cut-off 60%) G6PD deficiency was found in 206 volunteers. Median male activity was 6.1 U/g Hb. Eleven participants (5.4%) showed activities between 70 and 80%. No haemoglobinopathy was detected. None of the subjects showed asymptomatic parasitaemia. One G6PD-A+ variant (A376G) and one new non-synonymous mutation (G445A) were found. Conclusions As the prevalence of G6PD deficiency seems low in this area, the use of 8-aminoquinolines should be encouraged. However, reliable G6PD testing methods have to be implemented and safe cut-off levels need to be defined.http://link.springer.com/article/10.1186/s12936-018-2510-3 |
| spellingShingle | Nora Kießling Joaquin Brintrup Ahmed Zeynudin Nuredin Abduselam Sylvia Götz Margith Mack Michael Pritsch Andreas Wieser Elisabeth Kohne Nicole Berens-Riha Glucose-6-phosphate dehydrogenase activity measured by spectrophotometry and associated genetic variants from the Oromiya zone, Ethiopia Malaria Journal |
| title | Glucose-6-phosphate dehydrogenase activity measured by spectrophotometry and associated genetic variants from the Oromiya zone, Ethiopia |
| title_full | Glucose-6-phosphate dehydrogenase activity measured by spectrophotometry and associated genetic variants from the Oromiya zone, Ethiopia |
| title_fullStr | Glucose-6-phosphate dehydrogenase activity measured by spectrophotometry and associated genetic variants from the Oromiya zone, Ethiopia |
| title_full_unstemmed | Glucose-6-phosphate dehydrogenase activity measured by spectrophotometry and associated genetic variants from the Oromiya zone, Ethiopia |
| title_short | Glucose-6-phosphate dehydrogenase activity measured by spectrophotometry and associated genetic variants from the Oromiya zone, Ethiopia |
| title_sort | glucose 6 phosphate dehydrogenase activity measured by spectrophotometry and associated genetic variants from the oromiya zone ethiopia |
| url | http://link.springer.com/article/10.1186/s12936-018-2510-3 |
| work_keys_str_mv | AT norakießling glucose6phosphatedehydrogenaseactivitymeasuredbyspectrophotometryandassociatedgeneticvariantsfromtheoromiyazoneethiopia AT joaquinbrintrup glucose6phosphatedehydrogenaseactivitymeasuredbyspectrophotometryandassociatedgeneticvariantsfromtheoromiyazoneethiopia AT ahmedzeynudin glucose6phosphatedehydrogenaseactivitymeasuredbyspectrophotometryandassociatedgeneticvariantsfromtheoromiyazoneethiopia AT nuredinabduselam glucose6phosphatedehydrogenaseactivitymeasuredbyspectrophotometryandassociatedgeneticvariantsfromtheoromiyazoneethiopia AT sylviagotz glucose6phosphatedehydrogenaseactivitymeasuredbyspectrophotometryandassociatedgeneticvariantsfromtheoromiyazoneethiopia AT margithmack glucose6phosphatedehydrogenaseactivitymeasuredbyspectrophotometryandassociatedgeneticvariantsfromtheoromiyazoneethiopia AT michaelpritsch glucose6phosphatedehydrogenaseactivitymeasuredbyspectrophotometryandassociatedgeneticvariantsfromtheoromiyazoneethiopia AT andreaswieser glucose6phosphatedehydrogenaseactivitymeasuredbyspectrophotometryandassociatedgeneticvariantsfromtheoromiyazoneethiopia AT elisabethkohne glucose6phosphatedehydrogenaseactivitymeasuredbyspectrophotometryandassociatedgeneticvariantsfromtheoromiyazoneethiopia AT nicoleberensriha glucose6phosphatedehydrogenaseactivitymeasuredbyspectrophotometryandassociatedgeneticvariantsfromtheoromiyazoneethiopia |