Antibacterial effect of bioactive glass in combination with powdered enamel and dentin
<b>Background and Objectives:</b> In endodontics, various intracanal medications have been advocated to eliminate bacteria after root canal instrumentation. A recent study has revealed that addition of powdered dentin to bioactive glass (BAG) led to increased glass dissolution, and an i...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2010-01-01
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| Series: | Indian Journal of Dental Research |
| Subjects: | |
| Online Access: | http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=1;spage=30;epage=34;aulast=Prabhakar |
| _version_ | 1817979374086914048 |
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| author | Prabhakar A Kumar Santhosh |
| author_facet | Prabhakar A Kumar Santhosh |
| author_sort | Prabhakar A |
| collection | DOAJ |
| description | <b>Background and Objectives:</b> In endodontics, various intracanal medications have been advocated to eliminate bacteria after root canal instrumentation. A recent study has revealed that addition of powdered dentin to bioactive glass (BAG) led to increased glass dissolution, and an increased antibacterial efficacy. Therefore, the present study was undertaken to compare the effect of enamel and dentin powder on the antibacterial efficacy of a commercially available BAG. <b> Materials and Methods:</b> Dentin blocks (dbs) were prepared from single rooted human teeth. These dbs were infected with <i>Enterococcus</i> <i>faecalis</i> for two weeks in Tryptic Soy Broth (TSB), while negative controls were kept in sterile TSB. In group I, the infected dbs were filled with BAG, in group II with BAG + Enamel powder and group III with BAG + Dentin powder. Dentin samples were harvested from the dbs and cultured. <b> Statistical Analysis:</b> Kruskal-Wallis ANOVA was used for multiple group comparison followed by Scheffe′s post hoc test for pair-wise comparisons. <b> Results:</b> All the combinations of BAG evaluated significantly reduced the bacterial counts compared to the control group. However, at the end of 24 hours, three days, and five days BAG + Dentin powder showed significant reduction (<i> P</i> < 0.01) in bacterial counts compared to the other experimental groups. <b>Conclusion:</b> Among the various materials evaluated, it appeared that though BAG exhibits antimicrobial efficacy, the addition of powdered enamel and dentin in aqueous suspension definitely enhanced this property. However, the addition of enamel powder BAG did not significantly alter its antimicrobial efficacy compared to BAG + dentin powder. |
| first_indexed | 2024-04-13T22:41:06Z |
| format | Article |
| id | doaj.art-995d9db25a5048b38532792d4a3484af |
| institution | Directory Open Access Journal |
| issn | 0970-9290 1998-3603 |
| language | English |
| last_indexed | 2024-04-13T22:41:06Z |
| publishDate | 2010-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dental Research |
| spelling | doaj.art-995d9db25a5048b38532792d4a3484af2022-12-22T02:26:36ZengWolters Kluwer Medknow PublicationsIndian Journal of Dental Research0970-92901998-36032010-01-012113034Antibacterial effect of bioactive glass in combination with powdered enamel and dentinPrabhakar AKumar Santhosh<b>Background and Objectives:</b> In endodontics, various intracanal medications have been advocated to eliminate bacteria after root canal instrumentation. A recent study has revealed that addition of powdered dentin to bioactive glass (BAG) led to increased glass dissolution, and an increased antibacterial efficacy. Therefore, the present study was undertaken to compare the effect of enamel and dentin powder on the antibacterial efficacy of a commercially available BAG. <b> Materials and Methods:</b> Dentin blocks (dbs) were prepared from single rooted human teeth. These dbs were infected with <i>Enterococcus</i> <i>faecalis</i> for two weeks in Tryptic Soy Broth (TSB), while negative controls were kept in sterile TSB. In group I, the infected dbs were filled with BAG, in group II with BAG + Enamel powder and group III with BAG + Dentin powder. Dentin samples were harvested from the dbs and cultured. <b> Statistical Analysis:</b> Kruskal-Wallis ANOVA was used for multiple group comparison followed by Scheffe′s post hoc test for pair-wise comparisons. <b> Results:</b> All the combinations of BAG evaluated significantly reduced the bacterial counts compared to the control group. However, at the end of 24 hours, three days, and five days BAG + Dentin powder showed significant reduction (<i> P</i> < 0.01) in bacterial counts compared to the other experimental groups. <b>Conclusion:</b> Among the various materials evaluated, it appeared that though BAG exhibits antimicrobial efficacy, the addition of powdered enamel and dentin in aqueous suspension definitely enhanced this property. However, the addition of enamel powder BAG did not significantly alter its antimicrobial efficacy compared to BAG + dentin powder.http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=1;spage=30;epage=34;aulast=PrabhakarAntimicrobial efficacybioactive glassenamel powderdentin powderdentin blocksintracanal medicament |
| spellingShingle | Prabhakar A Kumar Santhosh Antibacterial effect of bioactive glass in combination with powdered enamel and dentin Indian Journal of Dental Research Antimicrobial efficacy bioactive glass enamel powder dentin powder dentin blocks intracanal medicament |
| title | Antibacterial effect of bioactive glass in combination with powdered enamel and dentin |
| title_full | Antibacterial effect of bioactive glass in combination with powdered enamel and dentin |
| title_fullStr | Antibacterial effect of bioactive glass in combination with powdered enamel and dentin |
| title_full_unstemmed | Antibacterial effect of bioactive glass in combination with powdered enamel and dentin |
| title_short | Antibacterial effect of bioactive glass in combination with powdered enamel and dentin |
| title_sort | antibacterial effect of bioactive glass in combination with powdered enamel and dentin |
| topic | Antimicrobial efficacy bioactive glass enamel powder dentin powder dentin blocks intracanal medicament |
| url | http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=1;spage=30;epage=34;aulast=Prabhakar |
| work_keys_str_mv | AT prabhakara antibacterialeffectofbioactiveglassincombinationwithpowderedenamelanddentin AT kumarsanthosh antibacterialeffectofbioactiveglassincombinationwithpowderedenamelanddentin |