The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability
3-Hydroxypropionaldehyde (3-HPA, reuterin) is a broad-spectrum natural antimicrobial agent used in the food industry and other fields. The low yield from the industrial production of 3-HPA using <i>Lactobacillus reuteri</i> and the spontaneous conversion of 3-HPA to acrolein have limited...
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MDPI AG
2021-09-01
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| author | Jung-Hyun Ju Sang-Gyu Jeon Kyung Min Lee Sun-Yeon Heo Min-Soo Kim Chul-Ho Kim Baek-Rock Oh |
| author_facet | Jung-Hyun Ju Sang-Gyu Jeon Kyung Min Lee Sun-Yeon Heo Min-Soo Kim Chul-Ho Kim Baek-Rock Oh |
| author_sort | Jung-Hyun Ju |
| collection | DOAJ |
| description | 3-Hydroxypropionaldehyde (3-HPA, reuterin) is a broad-spectrum natural antimicrobial agent used in the food industry and other fields. The low yield from the industrial production of 3-HPA using <i>Lactobacillus reuteri</i> and the spontaneous conversion of 3-HPA to acrolein have limited its more widespread use. We isolated <i>L. reuteri</i> BR201 as a biocatalyst for 3-HPA production and confirmed the effect of each factor in the two-step procedure for 3-HPA bioconversion. After initial cultivation for 8 h (late exponential phase), this isolate produced 378 mM of 3-HPA in 1 h at a concentration of OD<sub>600 nm</sub> 100, 30 °C, and an initial glycerol concentration of 500 mM. This is the highest reported biocatalytic yield of 3-HPA from a glycerol aqueous solution without additives. We confirmed that 4 mM of 3-HPA had antimicrobial activity against five pathogens. The degradation of 3-HPA to acrolein was greater at high temperatures, and there was little degradation when 3-HPA was maintained at 4 °C for 4 weeks. Our results may be useful for future applications of 3-HPA. |
| first_indexed | 2024-03-10T06:40:37Z |
| format | Article |
| id | doaj.art-99612a9d5f3f45b6b28db9c0886ad98a |
| institution | Directory Open Access Journal |
| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-10T06:40:37Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-99612a9d5f3f45b6b28db9c0886ad98a2023-11-22T17:42:48ZengMDPI AGCatalysts2073-43442021-09-011110113910.3390/catal11101139The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its StabilityJung-Hyun Ju0Sang-Gyu Jeon1Kyung Min Lee2Sun-Yeon Heo3Min-Soo Kim4Chul-Ho Kim5Baek-Rock Oh6Microbial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, KoreaMicrobial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, KoreaKolon Advanced Research Cluster, Kolon Industries, Gangseo, Seoul 07793, KoreaMicrobial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, KoreaMicrobial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, KoreaMicrobial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, KoreaMicrobial Biotechnology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56212, Korea3-Hydroxypropionaldehyde (3-HPA, reuterin) is a broad-spectrum natural antimicrobial agent used in the food industry and other fields. The low yield from the industrial production of 3-HPA using <i>Lactobacillus reuteri</i> and the spontaneous conversion of 3-HPA to acrolein have limited its more widespread use. We isolated <i>L. reuteri</i> BR201 as a biocatalyst for 3-HPA production and confirmed the effect of each factor in the two-step procedure for 3-HPA bioconversion. After initial cultivation for 8 h (late exponential phase), this isolate produced 378 mM of 3-HPA in 1 h at a concentration of OD<sub>600 nm</sub> 100, 30 °C, and an initial glycerol concentration of 500 mM. This is the highest reported biocatalytic yield of 3-HPA from a glycerol aqueous solution without additives. We confirmed that 4 mM of 3-HPA had antimicrobial activity against five pathogens. The degradation of 3-HPA to acrolein was greater at high temperatures, and there was little degradation when 3-HPA was maintained at 4 °C for 4 weeks. Our results may be useful for future applications of 3-HPA.https://www.mdpi.com/2073-4344/11/10/1139<i>Lactobacillus reuteri</i>3-hydroxypropionaldehydereuterinantibioticstability evaluation |
| spellingShingle | Jung-Hyun Ju Sang-Gyu Jeon Kyung Min Lee Sun-Yeon Heo Min-Soo Kim Chul-Ho Kim Baek-Rock Oh The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability Catalysts <i>Lactobacillus reuteri</i> 3-hydroxypropionaldehyde reuterin antibiotic stability evaluation |
| title | The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability |
| title_full | The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability |
| title_fullStr | The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability |
| title_full_unstemmed | The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability |
| title_short | The Biocatalytic Production of 3-Hydroxypropionaldehyde and Evaluation of Its Stability |
| title_sort | biocatalytic production of 3 hydroxypropionaldehyde and evaluation of its stability |
| topic | <i>Lactobacillus reuteri</i> 3-hydroxypropionaldehyde reuterin antibiotic stability evaluation |
| url | https://www.mdpi.com/2073-4344/11/10/1139 |
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