Longitudinal Gut Bacterial Colonization and Its Influencing Factors of Low Birth Weight Infants During the First 3 Months of Life
Establishment of low birth weight (LBW) infant gut microbiota may have lifelong implications for the health of individuals. However, no longitudinal cohort studies have been conducted to characterize the gut microbial profiles of LBW infants and their influencing factors. Our objective was to unders...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01105/full |
| _version_ | 1819173223093239808 |
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| author | Cheng Chi Yong Xue Yong Xue Na Lv Yanan Hao Ruixia Liu Yanxin Wang Xin Ding Huihui Zeng Geng Li Qun Shen Xiaosong Hu Lijun Chen Tiemin Jiang Junying Zhao Nicholas Buys Jing Sun Chenghong Yin Baoli Zhu Baoli Zhu Baoli Zhu Baoli Zhu |
| author_facet | Cheng Chi Yong Xue Yong Xue Na Lv Yanan Hao Ruixia Liu Yanxin Wang Xin Ding Huihui Zeng Geng Li Qun Shen Xiaosong Hu Lijun Chen Tiemin Jiang Junying Zhao Nicholas Buys Jing Sun Chenghong Yin Baoli Zhu Baoli Zhu Baoli Zhu Baoli Zhu |
| author_sort | Cheng Chi |
| collection | DOAJ |
| description | Establishment of low birth weight (LBW) infant gut microbiota may have lifelong implications for the health of individuals. However, no longitudinal cohort studies have been conducted to characterize the gut microbial profiles of LBW infants and their influencing factors. Our objective was to understand how the gut bacterial community structure of LBW and normal birth weight (NBW) infants varies across the first 3 months of life and assess the influencing factors. In this observational cohort study, gut bacterial composition was identified with sequencing of the 16S rRNA gene in fecal samples of 69 LBW infants and 65 NBW controls at 0 day, 3 days, 2 weeks, 6 weeks, and 3 months (defined as stages 1–5) after birth. Alpha-diversity of both groups displayed a decreasing trend followed by slight variations. There were significant differences on the Shannon index of the two groups at stages 1 to 3 (P = 0.041, P = 0.032, and P = 0.014, respectively). The microbiota community structure of LBW infants were significantly different from NBW infants throughout the 3 months (all P < 0.05) but not at stage 2 (P = 0.054). There was a significant increase in abundance in Firmicutes while a decrease in Proteobacteria, and at genus level the abundance of Enterococcus, Klebsiella, and Streptococcus increased while it decreased for Haemophilus in LBW group. Birth weight was the main factor explaining the observed variation at all stages, except at stage 2. Delivery mode (4.78%) and antibiotic usage (3.50%) contributed to explain the observed variation at stage 3, and pregestational BMI (4.61%) partially explained the observed variation at stage 4. In conclusion, gut microbial communities differed in NBW and LBW infants from birth to 3 months of life, and were affected by birth weight, delivery mode, antibiotic treatment, and pregestational BMI. |
| first_indexed | 2024-12-22T20:19:39Z |
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| id | doaj.art-9962b6470e824a70873cb4f59de1cb57 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T20:19:39Z |
| publishDate | 2019-05-01 |
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| spelling | doaj.art-9962b6470e824a70873cb4f59de1cb572022-12-21T18:13:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-05-011010.3389/fmicb.2019.01105434361Longitudinal Gut Bacterial Colonization and Its Influencing Factors of Low Birth Weight Infants During the First 3 Months of LifeCheng Chi0Yong Xue1Yong Xue2Na Lv3Yanan Hao4Ruixia Liu5Yanxin Wang6Xin Ding7Huihui Zeng8Geng Li9Qun Shen10Xiaosong Hu11Lijun Chen12Tiemin Jiang13Junying Zhao14Nicholas Buys15Jing Sun16Chenghong Yin17Baoli Zhu18Baoli Zhu19Baoli Zhu20Baoli Zhu21Department of Internal Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaDepartment of Central Laboratory, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, ChinaDepartment of Internal Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, ChinaDepartment of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, ChinaDepartment of Neonatology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, ChinaDepartment of Neonatology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaNational Engineering Center of Dairy for Early Life Health, Beijing Sanyuan Foods Co., Ltd., Beijing, ChinaNational Engineering Center of Dairy for Early Life Health, Beijing Sanyuan Foods Co., Ltd., Beijing, ChinaNational Engineering Center of Dairy for Early Life Health, Beijing Sanyuan Foods Co., Ltd., Beijing, ChinaSchool of Medicine, Griffith University, Brisbane, QLD, AustraliaSchool of Medicine, Griffith University, Brisbane, QLD, AustraliaDepartment of Internal Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaBeijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing, China0Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China1Department of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical University, Chengdu, ChinaEstablishment of low birth weight (LBW) infant gut microbiota may have lifelong implications for the health of individuals. However, no longitudinal cohort studies have been conducted to characterize the gut microbial profiles of LBW infants and their influencing factors. Our objective was to understand how the gut bacterial community structure of LBW and normal birth weight (NBW) infants varies across the first 3 months of life and assess the influencing factors. In this observational cohort study, gut bacterial composition was identified with sequencing of the 16S rRNA gene in fecal samples of 69 LBW infants and 65 NBW controls at 0 day, 3 days, 2 weeks, 6 weeks, and 3 months (defined as stages 1–5) after birth. Alpha-diversity of both groups displayed a decreasing trend followed by slight variations. There were significant differences on the Shannon index of the two groups at stages 1 to 3 (P = 0.041, P = 0.032, and P = 0.014, respectively). The microbiota community structure of LBW infants were significantly different from NBW infants throughout the 3 months (all P < 0.05) but not at stage 2 (P = 0.054). There was a significant increase in abundance in Firmicutes while a decrease in Proteobacteria, and at genus level the abundance of Enterococcus, Klebsiella, and Streptococcus increased while it decreased for Haemophilus in LBW group. Birth weight was the main factor explaining the observed variation at all stages, except at stage 2. Delivery mode (4.78%) and antibiotic usage (3.50%) contributed to explain the observed variation at stage 3, and pregestational BMI (4.61%) partially explained the observed variation at stage 4. In conclusion, gut microbial communities differed in NBW and LBW infants from birth to 3 months of life, and were affected by birth weight, delivery mode, antibiotic treatment, and pregestational BMI.https://www.frontiersin.org/article/10.3389/fmicb.2019.01105/fulllow birth weight infantgut microbiotaintestinal colonizationmicrobial diversityinfluencing factor |
| spellingShingle | Cheng Chi Yong Xue Yong Xue Na Lv Yanan Hao Ruixia Liu Yanxin Wang Xin Ding Huihui Zeng Geng Li Qun Shen Xiaosong Hu Lijun Chen Tiemin Jiang Junying Zhao Nicholas Buys Jing Sun Chenghong Yin Baoli Zhu Baoli Zhu Baoli Zhu Baoli Zhu Longitudinal Gut Bacterial Colonization and Its Influencing Factors of Low Birth Weight Infants During the First 3 Months of Life Frontiers in Microbiology low birth weight infant gut microbiota intestinal colonization microbial diversity influencing factor |
| title | Longitudinal Gut Bacterial Colonization and Its Influencing Factors of Low Birth Weight Infants During the First 3 Months of Life |
| title_full | Longitudinal Gut Bacterial Colonization and Its Influencing Factors of Low Birth Weight Infants During the First 3 Months of Life |
| title_fullStr | Longitudinal Gut Bacterial Colonization and Its Influencing Factors of Low Birth Weight Infants During the First 3 Months of Life |
| title_full_unstemmed | Longitudinal Gut Bacterial Colonization and Its Influencing Factors of Low Birth Weight Infants During the First 3 Months of Life |
| title_short | Longitudinal Gut Bacterial Colonization and Its Influencing Factors of Low Birth Weight Infants During the First 3 Months of Life |
| title_sort | longitudinal gut bacterial colonization and its influencing factors of low birth weight infants during the first 3 months of life |
| topic | low birth weight infant gut microbiota intestinal colonization microbial diversity influencing factor |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01105/full |
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