Chenopodium Ambrosioides Linn Mitigates Bone Loss in Rats with Periodontitis
Statement of the Problem: Periodontitis is an inflammatory disease that causes bone loss. Some patients do not respond well to the classic treatment and need therapies that minimize bone loss, the main sequel of the disease. Chenopodium ambrosioides L. has stood out due to its anti-inflammatory and...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shiraz University of Medical Sciences
2024-03-01
|
| Series: | Journal of Dentistry |
| Subjects: | |
| Online Access: | https://dentjods.sums.ac.ir/article_49295_907744a4047c5918373c3f408f5c30f6.pdf |
| _version_ | 1797277033126952960 |
|---|---|
| author | Diego Carneiro Michael da Silva Karla Vanessa Vasconcelos Romero Dias Vanessa Costa Raquel Vasconcelos Bárbara Carneiro Gisele Barreto Mirna Marques Hellíada Vasconcelos Howard Ribeiro Júnior Jonas Gusmão Helson da Silveira Renata Leitão Gerly Anne Brito Karuza Maria Pereira Delane Gondim Paula Goes |
| author_facet | Diego Carneiro Michael da Silva Karla Vanessa Vasconcelos Romero Dias Vanessa Costa Raquel Vasconcelos Bárbara Carneiro Gisele Barreto Mirna Marques Hellíada Vasconcelos Howard Ribeiro Júnior Jonas Gusmão Helson da Silveira Renata Leitão Gerly Anne Brito Karuza Maria Pereira Delane Gondim Paula Goes |
| author_sort | Diego Carneiro |
| collection | DOAJ |
| description | Statement of the Problem: Periodontitis is an inflammatory disease that causes bone loss. Some patients do not respond well to the classic treatment and need therapies that minimize bone loss, the main sequel of the disease. Chenopodium ambrosioides L. has stood out due to its anti-inflammatory and anti-oxidative activities. However, no study has yet investigated its effect on periodontitis. Purpose: This study aimed to evaluate the bone protective effect of Chenopodium ambrosioides L. (CAL) extract on ligature-induced periodontitis model in rats.Materials and Method: For this, a pre-clinical assay was performed, using male Wistar rats divided into 3 groups: Naive (N) (n=6), not submitted to any procedure; Saline (SAL) (n=6), submitted to ligature-induced periodontitis and receiving 2 ml/kg of 0.9% saline solution; and CAL extract, which was subdivided into 3 subgroups (n=6/subgroup) receiving the CAL at 3 (CAL3), 10 (CAL10) or 30 mg/kg (CAL30). All agents were given, by oral gavage, 30 min before periodontitis induction and daily until euthanasia (11th day). By then, maxillae were removed for macroscopic, histological, and histometric analyses. Kidneys, liver, and stomach were collected to evaluate the safety of CAL extract. High-performance liquid chromatography (HPLC) assay was used to investigate the flavonoid content in the extract. Results: Chenopodium ambrosioides L. extract at 30mg/kg showed a reduction by 58% in bone loss marked by an increase (+35%) in the number of osteoblasts and a reduction (-51%) on the number of osteoclasts (p< 0.05). No significant alteration in the liver, kidney, or stomach was seen. Rutin was the main flavonoid found.Conclusion: In summary, it was observed that Chenopodium ambrosioides L. extract has shown important anti-inflammatory and bone anabolic and anti-resorptive properties without causing toxicity in the main organs. Rutin, as the main flavonoid of the extract, seems to be responsible for the beneficial effect of this agent. |
| first_indexed | 2024-03-07T15:37:48Z |
| format | Article |
| id | doaj.art-99637b30e8414662ba4247d0fef263f7 |
| institution | Directory Open Access Journal |
| issn | 2345-6485 2345-6418 |
| language | English |
| last_indexed | 2024-03-07T15:37:48Z |
| publishDate | 2024-03-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Journal of Dentistry |
| spelling | doaj.art-99637b30e8414662ba4247d0fef263f72024-03-05T09:30:50ZengShiraz University of Medical SciencesJournal of Dentistry2345-64852345-64182024-03-01251596710.30476/dentjods.2023.95767.189149295Chenopodium Ambrosioides Linn Mitigates Bone Loss in Rats with PeriodontitisDiego Carneiro0Michael da Silva1Karla Vanessa Vasconcelos2Romero Dias3Vanessa Costa4Raquel Vasconcelos5Bárbara Carneiro6Gisele Barreto7Mirna Marques8Hellíada Vasconcelos9Howard Ribeiro Júnior10Jonas Gusmão11Helson da Silveira12Renata Leitão13Gerly Anne Brito14Karuza Maria Pereira15Delane Gondim16Paula Goes17Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Post Graduation Program of Dentistry, School of Dentistry, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Post Graduation Program in Health Science, School of Medicine, Federal University of Ceará, Sobral, Brazil.Post Graduation Program in Health Science, School of Medicine, Federal University of Ceará, Sobral, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Dept. of Pathology and Legal Medicine, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.Statement of the Problem: Periodontitis is an inflammatory disease that causes bone loss. Some patients do not respond well to the classic treatment and need therapies that minimize bone loss, the main sequel of the disease. Chenopodium ambrosioides L. has stood out due to its anti-inflammatory and anti-oxidative activities. However, no study has yet investigated its effect on periodontitis. Purpose: This study aimed to evaluate the bone protective effect of Chenopodium ambrosioides L. (CAL) extract on ligature-induced periodontitis model in rats.Materials and Method: For this, a pre-clinical assay was performed, using male Wistar rats divided into 3 groups: Naive (N) (n=6), not submitted to any procedure; Saline (SAL) (n=6), submitted to ligature-induced periodontitis and receiving 2 ml/kg of 0.9% saline solution; and CAL extract, which was subdivided into 3 subgroups (n=6/subgroup) receiving the CAL at 3 (CAL3), 10 (CAL10) or 30 mg/kg (CAL30). All agents were given, by oral gavage, 30 min before periodontitis induction and daily until euthanasia (11th day). By then, maxillae were removed for macroscopic, histological, and histometric analyses. Kidneys, liver, and stomach were collected to evaluate the safety of CAL extract. High-performance liquid chromatography (HPLC) assay was used to investigate the flavonoid content in the extract. Results: Chenopodium ambrosioides L. extract at 30mg/kg showed a reduction by 58% in bone loss marked by an increase (+35%) in the number of osteoblasts and a reduction (-51%) on the number of osteoclasts (p< 0.05). No significant alteration in the liver, kidney, or stomach was seen. Rutin was the main flavonoid found.Conclusion: In summary, it was observed that Chenopodium ambrosioides L. extract has shown important anti-inflammatory and bone anabolic and anti-resorptive properties without causing toxicity in the main organs. Rutin, as the main flavonoid of the extract, seems to be responsible for the beneficial effect of this agent.https://dentjods.sums.ac.ir/article_49295_907744a4047c5918373c3f408f5c30f6.pdfperiodontitisinflammationchenopodium ambrosioidesflavonoidsrutin |
| spellingShingle | Diego Carneiro Michael da Silva Karla Vanessa Vasconcelos Romero Dias Vanessa Costa Raquel Vasconcelos Bárbara Carneiro Gisele Barreto Mirna Marques Hellíada Vasconcelos Howard Ribeiro Júnior Jonas Gusmão Helson da Silveira Renata Leitão Gerly Anne Brito Karuza Maria Pereira Delane Gondim Paula Goes Chenopodium Ambrosioides Linn Mitigates Bone Loss in Rats with Periodontitis Journal of Dentistry periodontitis inflammation chenopodium ambrosioides flavonoids rutin |
| title | Chenopodium Ambrosioides Linn Mitigates Bone Loss in Rats with Periodontitis |
| title_full | Chenopodium Ambrosioides Linn Mitigates Bone Loss in Rats with Periodontitis |
| title_fullStr | Chenopodium Ambrosioides Linn Mitigates Bone Loss in Rats with Periodontitis |
| title_full_unstemmed | Chenopodium Ambrosioides Linn Mitigates Bone Loss in Rats with Periodontitis |
| title_short | Chenopodium Ambrosioides Linn Mitigates Bone Loss in Rats with Periodontitis |
| title_sort | chenopodium ambrosioides linn mitigates bone loss in rats with periodontitis |
| topic | periodontitis inflammation chenopodium ambrosioides flavonoids rutin |
| url | https://dentjods.sums.ac.ir/article_49295_907744a4047c5918373c3f408f5c30f6.pdf |
| work_keys_str_mv | AT diegocarneiro chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT michaeldasilva chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT karlavanessavasconcelos chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT romerodias chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT vanessacosta chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT raquelvasconcelos chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT barbaracarneiro chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT giselebarreto chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT mirnamarques chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT helliadavasconcelos chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT howardribeirojunior chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT jonasgusmao chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT helsondasilveira chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT renataleitao chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT gerlyannebrito chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT karuzamariapereira chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT delanegondim chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis AT paulagoes chenopodiumambrosioideslinnmitigatesbonelossinratswithperiodontitis |