Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome
Faecal microbiota transfer (FMT) consists of the introduction of new microbial communities into the intestine of a patient, with the aim of restoring a disturbed gut microbiota. Even though it is used as a potential treatment for various diseases, it is unknown how the host mucosa responds to FMT. T...
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MDPI AG
2019-10-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/10/586 |
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| author | Savanne Holster Guido J. Hooiveld Dirk Repsilber Willem M. de Vos Robert J. Brummer Julia König |
| author_facet | Savanne Holster Guido J. Hooiveld Dirk Repsilber Willem M. de Vos Robert J. Brummer Julia König |
| author_sort | Savanne Holster |
| collection | DOAJ |
| description | Faecal microbiota transfer (FMT) consists of the introduction of new microbial communities into the intestine of a patient, with the aim of restoring a disturbed gut microbiota. Even though it is used as a potential treatment for various diseases, it is unknown how the host mucosa responds to FMT. This study aims to investigate the colonic mucosa gene expression response to allogenic (from a donor) or autologous (own) FMT in patients with irritable bowel syndrome (IBS). In a recently conducted randomised, double-blinded, controlled clinical study, 17 IBS patients were treated with FMT by colonoscopy. RNA was isolated from colonic biopsies collected by sigmoidoscopy at baseline, as well as two weeks and eight weeks after FMT. In patients treated with allogenic FMT, predominantly immune response-related gene sets were induced, with the strongest response two weeks after the FMT. In patients treated with autologous FMT, predominantly metabolism-related gene sets were affected. Furthermore, several microbiota genera showed correlations with immune-related gene sets, with different correlations found after allogenic compared to autologous FMT. This study shows that the microbe−host response is influenced by FMT on the mucosal gene expression level, and that there are clear differences in response to allogenic compared to autologous FMT. |
| first_indexed | 2024-12-19T08:23:05Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-19T08:23:05Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj.art-996413598e3940849aa143d21fc2ff6d2022-12-21T20:29:22ZengMDPI AGBiomolecules2218-273X2019-10-0191058610.3390/biom9100586biom9100586Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel SyndromeSavanne Holster0Guido J. Hooiveld1Dirk Repsilber2Willem M. de Vos3Robert J. Brummer4Julia König5Nutrition-Gut-Brain Interactions Research Centre, Faculty of Health and Medicine, School of Medical Sciences, Örebro University, 701 82 Örebro, SwedenNutrition, Metabolism and Genomics group, Division of Human Nutrition and Health, Wageningen University, 6708 PB Wageningen, The NetherlandsNutrition-Gut-Brain Interactions Research Centre, Faculty of Health and Medicine, School of Medical Sciences, Örebro University, 701 82 Örebro, SwedenLaboratory of Microbiology, Wageningen University and Research Centre, 6708 PB Wageningen, The NetherlandsNutrition-Gut-Brain Interactions Research Centre, Faculty of Health and Medicine, School of Medical Sciences, Örebro University, 701 82 Örebro, SwedenNutrition-Gut-Brain Interactions Research Centre, Faculty of Health and Medicine, School of Medical Sciences, Örebro University, 701 82 Örebro, SwedenFaecal microbiota transfer (FMT) consists of the introduction of new microbial communities into the intestine of a patient, with the aim of restoring a disturbed gut microbiota. Even though it is used as a potential treatment for various diseases, it is unknown how the host mucosa responds to FMT. This study aims to investigate the colonic mucosa gene expression response to allogenic (from a donor) or autologous (own) FMT in patients with irritable bowel syndrome (IBS). In a recently conducted randomised, double-blinded, controlled clinical study, 17 IBS patients were treated with FMT by colonoscopy. RNA was isolated from colonic biopsies collected by sigmoidoscopy at baseline, as well as two weeks and eight weeks after FMT. In patients treated with allogenic FMT, predominantly immune response-related gene sets were induced, with the strongest response two weeks after the FMT. In patients treated with autologous FMT, predominantly metabolism-related gene sets were affected. Furthermore, several microbiota genera showed correlations with immune-related gene sets, with different correlations found after allogenic compared to autologous FMT. This study shows that the microbe−host response is influenced by FMT on the mucosal gene expression level, and that there are clear differences in response to allogenic compared to autologous FMT.https://www.mdpi.com/2218-273X/9/10/586faecal microbiota transplantationirritable bowel syndromegene expressionmicrobiotahost-microbe interaction |
| spellingShingle | Savanne Holster Guido J. Hooiveld Dirk Repsilber Willem M. de Vos Robert J. Brummer Julia König Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome Biomolecules faecal microbiota transplantation irritable bowel syndrome gene expression microbiota host-microbe interaction |
| title | Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome |
| title_full | Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome |
| title_fullStr | Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome |
| title_full_unstemmed | Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome |
| title_short | Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel Syndrome |
| title_sort | allogenic faecal microbiota transfer induces immune related gene sets in the colon mucosa of patients with irritable bowel syndrome |
| topic | faecal microbiota transplantation irritable bowel syndrome gene expression microbiota host-microbe interaction |
| url | https://www.mdpi.com/2218-273X/9/10/586 |
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