Lipid-lowering and anti-lipase properties of powder fractions of Dichrostachys glomerata fruits
Background: Fruits of Dichrostachys glomerata have in the last ten years benefited from special attention as a lipid-lowering plant. Recent studies show that biological activities of some plants depend on granulometry of their powder particles. Aims and Objective: The purpose of this study was to...
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| Format: | Article |
| Language: | English |
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Manipal College of Medical Sciences, Pokhara
2020-09-01
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| Series: | Asian Journal of Medical Sciences |
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| Online Access: | https://www.nepjol.info/index.php/AJMS/article/view/29229 |
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| author | Ngatchic Metsagang Therese Josiane Douanla Nodem Forestine Nikaise Ndjantou Elie Baudelaire Njintang Yanou Nicolas |
| author_facet | Ngatchic Metsagang Therese Josiane Douanla Nodem Forestine Nikaise Ndjantou Elie Baudelaire Njintang Yanou Nicolas |
| author_sort | Ngatchic Metsagang Therese Josiane |
| collection | DOAJ |
| description | Background: Fruits of Dichrostachys glomerata have in the last ten years benefited from special attention as a lipid-lowering plant. Recent studies show that biological activities of some plants depend on granulometry of their powder particles.
Aims and Objective: The purpose of this study was to assess antihyperlipidemic, hypolipidemic, and anti-lipase properties of powder fractions of the fruits of Dichrostachys glomerata.
Materials and Methods: The groups of rats on which the antihyperlipidemic test was done were fed with High Fat Diet and supplemented with powder fractions: ≥180μm, 212–180μm, 315–212μm, ≥315 μm and unsieved powder of Dichrostachys glomerata fruits at dose of 250 mg/kg for four weeks. For the hypolipidemic test, the diet was changed to normal diet and the powder fraction: 212 – 180μm, was given to rats for four weeks. Lipase inhibitory activity was determined using olive oil as substrate.
Results: The antihyperlipidemic test showed that powder fractions reduced levels of total cholesterol, LDL-Cholesterol and triglycerides, groups taken powder fractions 212 –180µm and <180µm, presented the lowest values. HDL-Cholesterol levels increased, significant increases were observed in groups fed with powder fractions 315–212µm, 212–180µm, <180µm and unsieved powder. Concerning hypolipidemic test, powder fraction 212–180 µm decreased levels of total cholesterol, LDL-Cholesterol and triglycerides. 86.43% of the activity of pancreatic lipase was reduced to a concentration of 25mg/ml with an IC50 of 5mg/ml.
Conclusion: Antihyperlipidemic properties of Dichrostachys glomerata fruits depended on powder fractions. Fraction 180-212μm exhibits hypolipidemic effects. The inhibition of pancreatic lipase is one of the mechanisms which could explain lipid- lowering properties of Dichrostachys glomerata fruits. |
| first_indexed | 2024-12-19T18:28:46Z |
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| id | doaj.art-996444b8efc74fa09c9ad5fdbd8964c0 |
| institution | Directory Open Access Journal |
| issn | 2467-9100 2091-0576 |
| language | English |
| last_indexed | 2024-12-19T18:28:46Z |
| publishDate | 2020-09-01 |
| publisher | Manipal College of Medical Sciences, Pokhara |
| record_format | Article |
| series | Asian Journal of Medical Sciences |
| spelling | doaj.art-996444b8efc74fa09c9ad5fdbd8964c02022-12-21T20:10:46ZengManipal College of Medical Sciences, PokharaAsian Journal of Medical Sciences2467-91002091-05762020-09-011156976https://doi.org/10.3126/ajms.v11i5.29229Lipid-lowering and anti-lipase properties of powder fractions of Dichrostachys glomerata fruitsNgatchic Metsagang Therese Josiane 0Douanla Nodem Forestine Nikaise 1Ndjantou Elie Baudelaire 2Njintang Yanou Nicolas 3Department of Food Science and Nutrition, National School of Agro-industrial Science, Ngaoundere, P.O. Box 455, University of Ngaoundere, Cameroon https://orcid.org/0000-0002-5385-5518Department of Food Science and Nutrition, National School of Agro-industrial Science, Ngaoundere, P.O. Box 455, University of Ngaoundere, CameroonDepartment of Food Science and Nutrition, National School of Agro-industrial Science, Ngaoundere, P.O. Box 455, University of Ngaoundere, Cameroon https://orcid.org/0000-0002-3699-3652Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere, Ngaoundere, Cameroon https://orcid.org/0000-0002-8949-7150Background: Fruits of Dichrostachys glomerata have in the last ten years benefited from special attention as a lipid-lowering plant. Recent studies show that biological activities of some plants depend on granulometry of their powder particles. Aims and Objective: The purpose of this study was to assess antihyperlipidemic, hypolipidemic, and anti-lipase properties of powder fractions of the fruits of Dichrostachys glomerata. Materials and Methods: The groups of rats on which the antihyperlipidemic test was done were fed with High Fat Diet and supplemented with powder fractions: ≥180μm, 212–180μm, 315–212μm, ≥315 μm and unsieved powder of Dichrostachys glomerata fruits at dose of 250 mg/kg for four weeks. For the hypolipidemic test, the diet was changed to normal diet and the powder fraction: 212 – 180μm, was given to rats for four weeks. Lipase inhibitory activity was determined using olive oil as substrate. Results: The antihyperlipidemic test showed that powder fractions reduced levels of total cholesterol, LDL-Cholesterol and triglycerides, groups taken powder fractions 212 –180µm and <180µm, presented the lowest values. HDL-Cholesterol levels increased, significant increases were observed in groups fed with powder fractions 315–212µm, 212–180µm, <180µm and unsieved powder. Concerning hypolipidemic test, powder fraction 212–180 µm decreased levels of total cholesterol, LDL-Cholesterol and triglycerides. 86.43% of the activity of pancreatic lipase was reduced to a concentration of 25mg/ml with an IC50 of 5mg/ml. Conclusion: Antihyperlipidemic properties of Dichrostachys glomerata fruits depended on powder fractions. Fraction 180-212μm exhibits hypolipidemic effects. The inhibition of pancreatic lipase is one of the mechanisms which could explain lipid- lowering properties of Dichrostachys glomerata fruits.https://www.nepjol.info/index.php/AJMS/article/view/29229dichrostachys glomeratafractions powderantihyperlipidemiahypolipidemiaanti-lipase |
| spellingShingle | Ngatchic Metsagang Therese Josiane Douanla Nodem Forestine Nikaise Ndjantou Elie Baudelaire Njintang Yanou Nicolas Lipid-lowering and anti-lipase properties of powder fractions of Dichrostachys glomerata fruits Asian Journal of Medical Sciences dichrostachys glomerata fractions powder antihyperlipidemia hypolipidemia anti-lipase |
| title | Lipid-lowering and anti-lipase properties of powder fractions of Dichrostachys glomerata fruits |
| title_full | Lipid-lowering and anti-lipase properties of powder fractions of Dichrostachys glomerata fruits |
| title_fullStr | Lipid-lowering and anti-lipase properties of powder fractions of Dichrostachys glomerata fruits |
| title_full_unstemmed | Lipid-lowering and anti-lipase properties of powder fractions of Dichrostachys glomerata fruits |
| title_short | Lipid-lowering and anti-lipase properties of powder fractions of Dichrostachys glomerata fruits |
| title_sort | lipid lowering and anti lipase properties of powder fractions of dichrostachys glomerata fruits |
| topic | dichrostachys glomerata fractions powder antihyperlipidemia hypolipidemia anti-lipase |
| url | https://www.nepjol.info/index.php/AJMS/article/view/29229 |
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