In Vivo Differentiation Potential of Epiblast Stem Cells Revealed by Chimeric Embryo Formation
Chimera formation after blastocyst injection or morula aggregation is the principal functional assay of the developmental potential of mouse embryonic stem cells (ESCs). This property, which demonstrates functional equivalence between ESCs and the preimplantation epiblast, is not shared by epiblast...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-12-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124712003774 |
| _version_ | 1818917890218262528 |
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| author | Yali Huang Rodrigo Osorno Anestis Tsakiridis Valerie Wilson |
| author_facet | Yali Huang Rodrigo Osorno Anestis Tsakiridis Valerie Wilson |
| author_sort | Yali Huang |
| collection | DOAJ |
| description | Chimera formation after blastocyst injection or morula aggregation is the principal functional assay of the developmental potential of mouse embryonic stem cells (ESCs). This property, which demonstrates functional equivalence between ESCs and the preimplantation epiblast, is not shared by epiblast stem cell (EpiSC) lines. Here, we show that EpiSCs derived either from postimplantation embryos or from ESCs in vitro readily generate chimeras when grafted to postimplantation embryos in whole embryo culture. EpiSC derivatives integrate and differentiate to derivatives of all three embryonic germ layers and primordial germ cells. In contrast, grafted ESCs seldom proliferate in postimplantation embryos, and fail to acquire the identity of their host-derived neighbors. EpiSCs do not incorporate efficiently into embryonic day 8.5 embryos, a stage by which pluripotency has been lost. Thus, chimera formation by EpiSCs requires a permissive environment, the postimplantation epiblast, and demonstrates functional equivalence between this cell type and EpiSCs. |
| first_indexed | 2024-12-20T00:41:15Z |
| format | Article |
| id | doaj.art-99667937109745bda795f2a5d4a86acd |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-20T00:41:15Z |
| publishDate | 2012-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-99667937109745bda795f2a5d4a86acd2022-12-21T19:59:35ZengElsevierCell Reports2211-12472012-12-01261571157810.1016/j.celrep.2012.10.022In Vivo Differentiation Potential of Epiblast Stem Cells Revealed by Chimeric Embryo FormationYali Huang0Rodrigo Osorno1Anestis Tsakiridis2Valerie Wilson3MRC Centre for Regenerative Medicine, School of Biological Sciences, SCRM Building, University of Edinburgh, Edinburgh bioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UKMRC Centre for Regenerative Medicine, School of Biological Sciences, SCRM Building, University of Edinburgh, Edinburgh bioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UKMRC Centre for Regenerative Medicine, School of Biological Sciences, SCRM Building, University of Edinburgh, Edinburgh bioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UKMRC Centre for Regenerative Medicine, School of Biological Sciences, SCRM Building, University of Edinburgh, Edinburgh bioQuarter, 5 Little France Drive, Edinburgh EH16 4UU, UKChimera formation after blastocyst injection or morula aggregation is the principal functional assay of the developmental potential of mouse embryonic stem cells (ESCs). This property, which demonstrates functional equivalence between ESCs and the preimplantation epiblast, is not shared by epiblast stem cell (EpiSC) lines. Here, we show that EpiSCs derived either from postimplantation embryos or from ESCs in vitro readily generate chimeras when grafted to postimplantation embryos in whole embryo culture. EpiSC derivatives integrate and differentiate to derivatives of all three embryonic germ layers and primordial germ cells. In contrast, grafted ESCs seldom proliferate in postimplantation embryos, and fail to acquire the identity of their host-derived neighbors. EpiSCs do not incorporate efficiently into embryonic day 8.5 embryos, a stage by which pluripotency has been lost. Thus, chimera formation by EpiSCs requires a permissive environment, the postimplantation epiblast, and demonstrates functional equivalence between this cell type and EpiSCs.http://www.sciencedirect.com/science/article/pii/S2211124712003774 |
| spellingShingle | Yali Huang Rodrigo Osorno Anestis Tsakiridis Valerie Wilson In Vivo Differentiation Potential of Epiblast Stem Cells Revealed by Chimeric Embryo Formation Cell Reports |
| title | In Vivo Differentiation Potential of Epiblast Stem Cells Revealed by Chimeric Embryo Formation |
| title_full | In Vivo Differentiation Potential of Epiblast Stem Cells Revealed by Chimeric Embryo Formation |
| title_fullStr | In Vivo Differentiation Potential of Epiblast Stem Cells Revealed by Chimeric Embryo Formation |
| title_full_unstemmed | In Vivo Differentiation Potential of Epiblast Stem Cells Revealed by Chimeric Embryo Formation |
| title_short | In Vivo Differentiation Potential of Epiblast Stem Cells Revealed by Chimeric Embryo Formation |
| title_sort | in vivo differentiation potential of epiblast stem cells revealed by chimeric embryo formation |
| url | http://www.sciencedirect.com/science/article/pii/S2211124712003774 |
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