Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiarities
Aphid species Aphis pomi (de Geer, 1773) is oligophagous on pomoideous host plants, whilst Aphis spiraecola Patch, 1914 is a polyphagous species alternating between Spiraea spp., its primary host, and a wide variety of secondary hosts, also including pomoideous species. Despite the biological distin...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2015-04-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-201502-0008_Aphis_pomi_and_Aphis_spiraecola_Hemiptera_Sternorrhyncha_Aphididae_in_Europe_-_new_information_on_their_dis.php |
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| author | Rimantas RAKAUSKAS Jekaterina BAŠILOVA Rasa BERNOTIENĖ |
| author_facet | Rimantas RAKAUSKAS Jekaterina BAŠILOVA Rasa BERNOTIENĖ |
| author_sort | Rimantas RAKAUSKAS |
| collection | DOAJ |
| description | Aphid species Aphis pomi (de Geer, 1773) is oligophagous on pomoideous host plants, whilst Aphis spiraecola Patch, 1914 is a polyphagous species alternating between Spiraea spp., its primary host, and a wide variety of secondary hosts, also including pomoideous species. Despite the biological distinction, these species are difficult to separate using their morphological characters. Partial sequences of mitochondrial COI and nuclear EF-1α genes were analyzed for samples from Central and Eastern Europe, Germany, Bulgaria, Italy, Turkey, China together with available data from GenBank. Interspecific pairwise sample divergences of the COI fragment ranged from 3.1 to 4.3%. One COI haplotype of A. pomi was predominant (n = 24), with a pan European distribution. The most abundant COI haplotype of A. spiraecola (n = 16) occurred in Lithuania, Latvia, Poland, Italy, Turkey and China. Interspecific pairwise sample divergences of the EF-1α fragment ranged from 0.6 to 1.2%. Analyzed partial sequences of EF-1α were identical in A. pomi. The most abundant EF-1α haplotype of A. spiraecola (n = 14) occurred in Lithuania, Poland, Italy, Turkey and China. The length of ultimate rostral segment appeared to be the most reliable morphological character for discrimination between apple and spirea aphid species. It allowed a 100% correct identification of A. pomi (n = 143) and 91.5% of A. spiraecola (n = 94) specimens in the European samples used for the molecular analysis. The existence of A. spiraecola in the Eastern Baltic region of Europe is documented for the first time. |
| first_indexed | 2024-12-14T21:09:51Z |
| format | Article |
| id | doaj.art-996a844455024c54868579bc62747ac8 |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-14T21:09:51Z |
| publishDate | 2015-04-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-996a844455024c54868579bc62747ac82022-12-21T22:47:17ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292015-04-01112227028010.14411/eje.2015.043eje-201502-0008Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiaritiesRimantas RAKAUSKAS0Jekaterina BAŠILOVA1Rasa BERNOTIENĖ2Department of Zoology, Faculty of Natural Sciences, Vilnius University, Čiurlionio str. 21/27, LT 03101 Vilnius, Lithuania; e-mails: rimantas.rakauskas@gf.vu.lt; jekaterina.basilova@gf.vu.lt; rasa.bernotiene@gf.vu.ltDepartment of Zoology, Faculty of Natural Sciences, Vilnius University, Čiurlionio str. 21/27, LT 03101 Vilnius, Lithuania; e-mails: rimantas.rakauskas@gf.vu.lt; jekaterina.basilova@gf.vu.lt; rasa.bernotiene@gf.vu.ltDepartment of Zoology, Faculty of Natural Sciences, Vilnius University, Čiurlionio str. 21/27, LT 03101 Vilnius, Lithuania; e-mails: rimantas.rakauskas@gf.vu.lt; jekaterina.basilova@gf.vu.lt; rasa.bernotiene@gf.vu.ltAphid species Aphis pomi (de Geer, 1773) is oligophagous on pomoideous host plants, whilst Aphis spiraecola Patch, 1914 is a polyphagous species alternating between Spiraea spp., its primary host, and a wide variety of secondary hosts, also including pomoideous species. Despite the biological distinction, these species are difficult to separate using their morphological characters. Partial sequences of mitochondrial COI and nuclear EF-1α genes were analyzed for samples from Central and Eastern Europe, Germany, Bulgaria, Italy, Turkey, China together with available data from GenBank. Interspecific pairwise sample divergences of the COI fragment ranged from 3.1 to 4.3%. One COI haplotype of A. pomi was predominant (n = 24), with a pan European distribution. The most abundant COI haplotype of A. spiraecola (n = 16) occurred in Lithuania, Latvia, Poland, Italy, Turkey and China. Interspecific pairwise sample divergences of the EF-1α fragment ranged from 0.6 to 1.2%. Analyzed partial sequences of EF-1α were identical in A. pomi. The most abundant EF-1α haplotype of A. spiraecola (n = 14) occurred in Lithuania, Poland, Italy, Turkey and China. The length of ultimate rostral segment appeared to be the most reliable morphological character for discrimination between apple and spirea aphid species. It allowed a 100% correct identification of A. pomi (n = 143) and 91.5% of A. spiraecola (n = 94) specimens in the European samples used for the molecular analysis. The existence of A. spiraecola in the Eastern Baltic region of Europe is documented for the first time.https://www.eje.cz/artkey/eje-201502-0008_Aphis_pomi_and_Aphis_spiraecola_Hemiptera_Sternorrhyncha_Aphididae_in_Europe_-_new_information_on_their_dis.phphemipteraaphididaeaphis pomiaphis spiraecolaapple aphidspirea aphidmorphometricsidentificationmitochondrial coinuclear ef-1α |
| spellingShingle | Rimantas RAKAUSKAS Jekaterina BAŠILOVA Rasa BERNOTIENĖ Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiarities European Journal of Entomology hemiptera aphididae aphis pomi aphis spiraecola apple aphid spirea aphid morphometrics identification mitochondrial coi nuclear ef-1α |
| title | Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiarities |
| title_full | Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiarities |
| title_fullStr | Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiarities |
| title_full_unstemmed | Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiarities |
| title_short | Aphis pomi and Aphis spiraecola (Hemiptera: Sternorrhyncha: Aphididae) in Europe - new information on their distribution, molecular and morphological peculiarities |
| title_sort | aphis pomi and aphis spiraecola hemiptera sternorrhyncha aphididae in europe new information on their distribution molecular and morphological peculiarities |
| topic | hemiptera aphididae aphis pomi aphis spiraecola apple aphid spirea aphid morphometrics identification mitochondrial coi nuclear ef-1α |
| url | https://www.eje.cz/artkey/eje-201502-0008_Aphis_pomi_and_Aphis_spiraecola_Hemiptera_Sternorrhyncha_Aphididae_in_Europe_-_new_information_on_their_dis.php |
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