The preparation of an infectious full-length cDNA clone of Saffold virus
<p>Abstract</p> <p>The pathogenicity of Saffold virus (SAFV) among humans still remains unclear, although it was identified as a novel human cardiovirus in 2007. In order to encourage the molecular pathogenetic studies of SAFV, we generated an infectious cDNA clone of SAFV type 3 (...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-03-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/8/1/110 |
| _version_ | 1819155834578403328 |
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| author | Okuwa Takako Shimizu Hiroyuki Asif Naeem Hosomi Takushi Himeda Toshiki Muraki Yasushi Ohara Yoshiro |
| author_facet | Okuwa Takako Shimizu Hiroyuki Asif Naeem Hosomi Takushi Himeda Toshiki Muraki Yasushi Ohara Yoshiro |
| author_sort | Okuwa Takako |
| collection | DOAJ |
| description | <p>Abstract</p> <p>The pathogenicity of Saffold virus (SAFV) among humans still remains unclear, although it was identified as a novel human cardiovirus in 2007. In order to encourage the molecular pathogenetic studies of SAFV, we generated an infectious cDNA clone of SAFV type 3 (SAFV-3). The present study demonstrated that the synthesis of the full-length infectious RNA by T7 RNA polymerase was terminated by a homologous sequence motif with the human preproparathyroid hormone (PTH) signal in the SAFV-3 genome. To obtain the infectious RNA using T7 promoter, a variant of T7 RNA polymerase, which fails to recognize the PTH signal, was useful. This study will provide a valuable technical insight into the reverse genetics of SAFV.</p> |
| first_indexed | 2024-12-22T15:43:16Z |
| format | Article |
| id | doaj.art-996c02b58c6040118eb397c2512210fd |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-22T15:43:16Z |
| publishDate | 2011-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-996c02b58c6040118eb397c2512210fd2022-12-21T18:21:06ZengBMCVirology Journal1743-422X2011-03-018111010.1186/1743-422X-8-110The preparation of an infectious full-length cDNA clone of Saffold virusOkuwa TakakoShimizu HiroyukiAsif NaeemHosomi TakushiHimeda ToshikiMuraki YasushiOhara Yoshiro<p>Abstract</p> <p>The pathogenicity of Saffold virus (SAFV) among humans still remains unclear, although it was identified as a novel human cardiovirus in 2007. In order to encourage the molecular pathogenetic studies of SAFV, we generated an infectious cDNA clone of SAFV type 3 (SAFV-3). The present study demonstrated that the synthesis of the full-length infectious RNA by T7 RNA polymerase was terminated by a homologous sequence motif with the human preproparathyroid hormone (PTH) signal in the SAFV-3 genome. To obtain the infectious RNA using T7 promoter, a variant of T7 RNA polymerase, which fails to recognize the PTH signal, was useful. This study will provide a valuable technical insight into the reverse genetics of SAFV.</p>http://www.virologyj.com/content/8/1/110 |
| spellingShingle | Okuwa Takako Shimizu Hiroyuki Asif Naeem Hosomi Takushi Himeda Toshiki Muraki Yasushi Ohara Yoshiro The preparation of an infectious full-length cDNA clone of Saffold virus Virology Journal |
| title | The preparation of an infectious full-length cDNA clone of Saffold virus |
| title_full | The preparation of an infectious full-length cDNA clone of Saffold virus |
| title_fullStr | The preparation of an infectious full-length cDNA clone of Saffold virus |
| title_full_unstemmed | The preparation of an infectious full-length cDNA clone of Saffold virus |
| title_short | The preparation of an infectious full-length cDNA clone of Saffold virus |
| title_sort | preparation of an infectious full length cdna clone of saffold virus |
| url | http://www.virologyj.com/content/8/1/110 |
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