An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat
EST-SSR markers, derived from the A and B genomes of wheat were used to identify molecular markers associated with yellow rust resistance. For this purpose, bulk segregant analysis was performed using 114 EST-SSR primer pairs. They were screened on the parent genotypes and resistant/susceptible DNA...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2014-03-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201401-0003_an-est-ssr-marker-bu099658-and-its-potential-use-in-breeding-for-yellow-rust-resistance-in-wheat.php |
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| author | Semra HASANCEBI Zafer MERT Fahriye ERTUGRUL Kadir AKAN Yildiz AYDIN Funda SENTURK AKFIRAT Ahu ALTINKUT UNCUOGLU |
| author_facet | Semra HASANCEBI Zafer MERT Fahriye ERTUGRUL Kadir AKAN Yildiz AYDIN Funda SENTURK AKFIRAT Ahu ALTINKUT UNCUOGLU |
| author_sort | Semra HASANCEBI |
| collection | DOAJ |
| description | EST-SSR markers, derived from the A and B genomes of wheat were used to identify molecular markers associated with yellow rust resistance. For this purpose, bulk segregant analysis was performed using 114 EST-SSR primer pairs. They were screened on the parent genotypes and resistant/susceptible DNA pools from the cross between Izgi2001 (resistant male parent) × ES14 (susceptible female parent) at the seedling and adult plant stage. An EST-SSR marker, bu099658, generated the 206 bp DNA fragment that was present in the resistant parent and resistant bulk, but it was not present in the susceptible parent and the susceptible bulk. To investigate its association with Yr genes, 20 individuals of NILs were also amplified with BU099658 and the 206 bp marker fragment was obtained only in Yr1/6 × Avocet S. Additionally, bu099658 was screened on 65 genotypes which possessed different Yr genes/gene combination(s) and Yr1. The results indicate a close linkage of bu099658 with the Yr1 gene. |
| first_indexed | 2024-04-10T08:29:35Z |
| format | Article |
| id | doaj.art-996d5c2fde3e49e790fea136261a2716 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:35Z |
| publishDate | 2014-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-996d5c2fde3e49e790fea136261a27162023-02-23T03:30:25ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252014-03-01501111810.17221/109/2013-CJGPBcjg-201401-0003An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheatSemra HASANCEBI0Zafer MERT1Fahriye ERTUGRUL2Kadir AKAN3Yildiz AYDIN4Funda SENTURK AKFIRAT5Ahu ALTINKUT UNCUOGLU6Genetic Engineering and Biotechnology Institute (GEBI), Marmara Research Center (MRC), Scientific and Technological Research Council of Turkey (TUBITAK), Kocaeli, TurkeyField Crop Research Institute, Ankara, TurkeyAgriculture, Forestry and Veterinary Research Group, Scientific and Technological Research Council of Turkey (TUBITAK), Ankara, TurkeyField Crop Research Institute, Ankara, TurkeyDepartment of Biology, Faculty of Arts and Science, Marmara University, Istanbul, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Science, Gebze Institute of Technology, Kocaeli, TurkeyDepartment of Bioengineering, Faculty of Engineering, Marmara University, Istanbul, TurkeyEST-SSR markers, derived from the A and B genomes of wheat were used to identify molecular markers associated with yellow rust resistance. For this purpose, bulk segregant analysis was performed using 114 EST-SSR primer pairs. They were screened on the parent genotypes and resistant/susceptible DNA pools from the cross between Izgi2001 (resistant male parent) × ES14 (susceptible female parent) at the seedling and adult plant stage. An EST-SSR marker, bu099658, generated the 206 bp DNA fragment that was present in the resistant parent and resistant bulk, but it was not present in the susceptible parent and the susceptible bulk. To investigate its association with Yr genes, 20 individuals of NILs were also amplified with BU099658 and the 206 bp marker fragment was obtained only in Yr1/6 × Avocet S. Additionally, bu099658 was screened on 65 genotypes which possessed different Yr genes/gene combination(s) and Yr1. The results indicate a close linkage of bu099658 with the Yr1 gene.https://cjgpb.agriculturejournals.cz/artkey/cjg-201401-0003_an-est-ssr-marker-bu099658-and-its-potential-use-in-breeding-for-yellow-rust-resistance-in-wheat.phpbulk segregant analysis (bsa)marker-assisted selection (mas)puccinia striiformis f.sp. triticitriticum aestivum l.yr1 |
| spellingShingle | Semra HASANCEBI Zafer MERT Fahriye ERTUGRUL Kadir AKAN Yildiz AYDIN Funda SENTURK AKFIRAT Ahu ALTINKUT UNCUOGLU An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat Czech Journal of Genetics and Plant Breeding bulk segregant analysis (bsa) marker-assisted selection (mas) puccinia striiformis f.sp. tritici triticum aestivum l. yr1 |
| title | An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat |
| title_full | An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat |
| title_fullStr | An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat |
| title_full_unstemmed | An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat |
| title_short | An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat |
| title_sort | est ssr marker bu099658 and its potential use in breeding for yellow rust resistance in wheat |
| topic | bulk segregant analysis (bsa) marker-assisted selection (mas) puccinia striiformis f.sp. tritici triticum aestivum l. yr1 |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201401-0003_an-est-ssr-marker-bu099658-and-its-potential-use-in-breeding-for-yellow-rust-resistance-in-wheat.php |
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