DNAzyme Sensor for the Detection of Ca<sup>2+</sup> Using Resistive Pulse Sensing
DNAzymes are DNA oligonucleotides that can undergo a specific chemical reaction in the presence of a cofactor. Ribonucleases are a specific form of DNAzymes where a tertiary structure undergoes cleavage at a single ribonuclease site. The cleavage is highly specificity to co-factors, which makes them...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/20/20/5877 |
| _version_ | 1797550670798127104 |
|---|---|
| author | Imogen Heaton Mark Platt |
| author_facet | Imogen Heaton Mark Platt |
| author_sort | Imogen Heaton |
| collection | DOAJ |
| description | DNAzymes are DNA oligonucleotides that can undergo a specific chemical reaction in the presence of a cofactor. Ribonucleases are a specific form of DNAzymes where a tertiary structure undergoes cleavage at a single ribonuclease site. The cleavage is highly specificity to co-factors, which makes them excellent sensor recognition elements. Monitoring the change in structure upon cleavage has given rise to many sensing strategies; here we present a simple and rapid method of following the reaction using resistive pulse sensors, RPS. To demonstrate this methodology, we present a sensor for Ca<sup>2+</sup> ions in solution. A nanoparticle was functionalised with a Ca<sup>2+</sup> DNAzyme, and it was possible to follow the cleavage and rearrangement of the DNA as the particles translocate the RPS. The binding of Ca<sup>2+</sup> caused a conformation change in the DNAzyme, which was monitored as a change in translocation speed. A 30 min assay produced a linear response for Ca<sup>2+</sup> between 1–9 μm, and extending the incubation time to 60 min allowed for a concentration as low as 0.3 μm. We demonstrate that the signal is specific to Ca<sup>2+</sup> in the presence of other metal ions, and we can quantify Ca<sup>2+</sup> in tap and pond water samples. |
| first_indexed | 2024-03-10T15:32:40Z |
| format | Article |
| id | doaj.art-9971d46c15554c26b4f14df413ec6819 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T15:32:40Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-9971d46c15554c26b4f14df413ec68192023-11-20T17:29:36ZengMDPI AGSensors1424-82202020-10-012020587710.3390/s20205877DNAzyme Sensor for the Detection of Ca<sup>2+</sup> Using Resistive Pulse SensingImogen Heaton0Mark Platt1Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, UKDepartment of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, UKDNAzymes are DNA oligonucleotides that can undergo a specific chemical reaction in the presence of a cofactor. Ribonucleases are a specific form of DNAzymes where a tertiary structure undergoes cleavage at a single ribonuclease site. The cleavage is highly specificity to co-factors, which makes them excellent sensor recognition elements. Monitoring the change in structure upon cleavage has given rise to many sensing strategies; here we present a simple and rapid method of following the reaction using resistive pulse sensors, RPS. To demonstrate this methodology, we present a sensor for Ca<sup>2+</sup> ions in solution. A nanoparticle was functionalised with a Ca<sup>2+</sup> DNAzyme, and it was possible to follow the cleavage and rearrangement of the DNA as the particles translocate the RPS. The binding of Ca<sup>2+</sup> caused a conformation change in the DNAzyme, which was monitored as a change in translocation speed. A 30 min assay produced a linear response for Ca<sup>2+</sup> between 1–9 μm, and extending the incubation time to 60 min allowed for a concentration as low as 0.3 μm. We demonstrate that the signal is specific to Ca<sup>2+</sup> in the presence of other metal ions, and we can quantify Ca<sup>2+</sup> in tap and pond water samples.https://www.mdpi.com/1424-8220/20/20/5877DNAzymeaptamernanoporeresistive pulse sensormetal ion sensor |
| spellingShingle | Imogen Heaton Mark Platt DNAzyme Sensor for the Detection of Ca<sup>2+</sup> Using Resistive Pulse Sensing Sensors DNAzyme aptamer nanopore resistive pulse sensor metal ion sensor |
| title | DNAzyme Sensor for the Detection of Ca<sup>2+</sup> Using Resistive Pulse Sensing |
| title_full | DNAzyme Sensor for the Detection of Ca<sup>2+</sup> Using Resistive Pulse Sensing |
| title_fullStr | DNAzyme Sensor for the Detection of Ca<sup>2+</sup> Using Resistive Pulse Sensing |
| title_full_unstemmed | DNAzyme Sensor for the Detection of Ca<sup>2+</sup> Using Resistive Pulse Sensing |
| title_short | DNAzyme Sensor for the Detection of Ca<sup>2+</sup> Using Resistive Pulse Sensing |
| title_sort | dnazyme sensor for the detection of ca sup 2 sup using resistive pulse sensing |
| topic | DNAzyme aptamer nanopore resistive pulse sensor metal ion sensor |
| url | https://www.mdpi.com/1424-8220/20/20/5877 |
| work_keys_str_mv | AT imogenheaton dnazymesensorforthedetectionofcasup2supusingresistivepulsesensing AT markplatt dnazymesensorforthedetectionofcasup2supusingresistivepulsesensing |