Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification
IntroductionAtractylodes lancea is widely distributed in East Asia, ranging from Amur to south-central China. The rhizome of A. lancea is commonly used in traditional Chinese medicine, however, the quality of products varies across different regions with different geochemical characteristics.MethodT...
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1237800/full |
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| author | Chengcai Zhang Hongyang Wang Chaogeng Lyu Yiheng Wang Jiahui Sun Yan Zhang Zengxu Xiang Xiuzhi Guo Yuefeng Wang Ming Qin Sheng Wang Sheng Wang Lanping Guo |
| author_facet | Chengcai Zhang Hongyang Wang Chaogeng Lyu Yiheng Wang Jiahui Sun Yan Zhang Zengxu Xiang Xiuzhi Guo Yuefeng Wang Ming Qin Sheng Wang Sheng Wang Lanping Guo |
| author_sort | Chengcai Zhang |
| collection | DOAJ |
| description | IntroductionAtractylodes lancea is widely distributed in East Asia, ranging from Amur to south-central China. The rhizome of A. lancea is commonly used in traditional Chinese medicine, however, the quality of products varies across different regions with different geochemical characteristics.MethodThis study aimed to identify the chemotypes of A. lancea from different areas and screen for chemical markers by quantifying volatile organic compounds (VOCs) using a targeted metabolomics approach based on GC–MS/MS.ResultsThe A. lancea distributed in Hubei, Anhui, Shaanxi, and a region west of Henan province was classified as the Hubei Chemotype (HBA). HBA is characterized by high content of β-eudesmol and hinesol with lower levels of atractylodin and atractylon. In contrast, the Maoshan Chemotype (MA) from Jiangsu, Shandong, Shanxi, Hebei, Inner Mongolia, and other northern regions, exhibited high levels of atractylodin and atractylon. A total of 15 categories of VOCs metabolites were detected and identified, revealing significant differences in the profiles of terpenoid, heterocyclic compound, ester, and ketone among different areas. Multivariate statistics indicated that 6 compounds and 455 metabolites could serve as candidate markers for differentiating A. lancea obtained from the southern, northern, and Maoshan areas.DiscussionThis comprehensive analysis provides a chemical fingerprint of selected A. lancea. Our results highlight the potential of metabolite profiling combined with chemometrics for authenticating the geographical origin of A. lancea. |
| first_indexed | 2024-03-11T21:15:08Z |
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| language | English |
| last_indexed | 2024-03-11T21:15:08Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-99725dbb3829438facf895deb418e36a2023-09-29T04:52:22ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-09-011410.3389/fpls.2023.12378001237800Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identificationChengcai Zhang0Hongyang Wang1Chaogeng Lyu2Yiheng Wang3Jiahui Sun4Yan Zhang5Zengxu Xiang6Xiuzhi Guo7Yuefeng Wang8Ming Qin9Sheng Wang10Sheng Wang11Lanping Guo12State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaCollege of Horticulture of Nanjing Agricultural University, Nanjing, ChinaState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaDexing Research and Training Center of Chinese Medical Sciences, China Academy of Chinese Medical Science, Dexing, ChinaState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaDexing Research and Training Center of Chinese Medical Sciences, China Academy of Chinese Medical Science, Dexing, ChinaState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaIntroductionAtractylodes lancea is widely distributed in East Asia, ranging from Amur to south-central China. The rhizome of A. lancea is commonly used in traditional Chinese medicine, however, the quality of products varies across different regions with different geochemical characteristics.MethodThis study aimed to identify the chemotypes of A. lancea from different areas and screen for chemical markers by quantifying volatile organic compounds (VOCs) using a targeted metabolomics approach based on GC–MS/MS.ResultsThe A. lancea distributed in Hubei, Anhui, Shaanxi, and a region west of Henan province was classified as the Hubei Chemotype (HBA). HBA is characterized by high content of β-eudesmol and hinesol with lower levels of atractylodin and atractylon. In contrast, the Maoshan Chemotype (MA) from Jiangsu, Shandong, Shanxi, Hebei, Inner Mongolia, and other northern regions, exhibited high levels of atractylodin and atractylon. A total of 15 categories of VOCs metabolites were detected and identified, revealing significant differences in the profiles of terpenoid, heterocyclic compound, ester, and ketone among different areas. Multivariate statistics indicated that 6 compounds and 455 metabolites could serve as candidate markers for differentiating A. lancea obtained from the southern, northern, and Maoshan areas.DiscussionThis comprehensive analysis provides a chemical fingerprint of selected A. lancea. Our results highlight the potential of metabolite profiling combined with chemometrics for authenticating the geographical origin of A. lancea.https://www.frontiersin.org/articles/10.3389/fpls.2023.1237800/fullAtractylodes lanceaGC-MSchemotypeVOCmetabolomic profilingchemical markers |
| spellingShingle | Chengcai Zhang Hongyang Wang Chaogeng Lyu Yiheng Wang Jiahui Sun Yan Zhang Zengxu Xiang Xiuzhi Guo Yuefeng Wang Ming Qin Sheng Wang Sheng Wang Lanping Guo Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification Frontiers in Plant Science Atractylodes lancea GC-MS chemotype VOC metabolomic profiling chemical markers |
| title | Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification |
| title_full | Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification |
| title_fullStr | Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification |
| title_full_unstemmed | Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification |
| title_short | Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification |
| title_sort | authenticating the geographic origins of atractylodes lancea rhizome chemotypes in china through metabolite marker identification |
| topic | Atractylodes lancea GC-MS chemotype VOC metabolomic profiling chemical markers |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1237800/full |
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