Immunohistochemical localization of d‐β‐aspartic acid in congenital and acquired middle ear cholesteatoma
Abstract Objective/Hypothesis Middle ear cholesteatoma is characterized by abnormal growth of the keratinizing squamous epithelium of the temporal bone. d‐β‐aspartic acid is the major isomer of d‐aspartic acid found in elderly tissue. We assessed the immunoreactivity to k‐β‐aspartic acid of congenit...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-08-01
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| Series: | Laryngoscope Investigative Otolaryngology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/lio2.856 |
| _version_ | 1828358060431638528 |
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| author | Shiori Kitaya Ryoukichi Ikeda Jun Suzuki Hidetoshi Oshima Yuri Nomura Yusuke Kusano Nobuo Ohta Tetsuaki Kawase Kazue Ise Kazuhiro Murakami Yasuhiro Nakamura Hironobu Sasano Yukio Katori |
| author_facet | Shiori Kitaya Ryoukichi Ikeda Jun Suzuki Hidetoshi Oshima Yuri Nomura Yusuke Kusano Nobuo Ohta Tetsuaki Kawase Kazue Ise Kazuhiro Murakami Yasuhiro Nakamura Hironobu Sasano Yukio Katori |
| author_sort | Shiori Kitaya |
| collection | DOAJ |
| description | Abstract Objective/Hypothesis Middle ear cholesteatoma is characterized by abnormal growth of the keratinizing squamous epithelium of the temporal bone. d‐β‐aspartic acid is the major isomer of d‐aspartic acid found in elderly tissue. We assessed the immunoreactivity to k‐β‐aspartic acid of congenital and acquired middle ear cholesteatomas. Study Design Case–control studies. Material and Methods Tissue samples were collected from 21 patients comprising 21 ears with congenital middle ear cholesteatoma and 26 patients comprising 29 ears with acquired type. Their clinical and histopathological features were investigated. We divided the middle ear cholesteatoma samples into three layers: the perimatrix, matrix, and cystic contents. The patterns of immunoreactivity to d‐β‐aspartic acid expression were then assessed immunohistochemically. Results Two patterns of immunoreactivity to d‐β‐aspartic acid were detected in middle ear cholesteatoma: infiltrative and diffuse. In congenital middle ear cholesteatoma, d‐β‐aspartic acid expression was observed throughout all the layers (perimatrix, matrix, and cystic contents), and immunoreactivity to d‐β‐aspartic acid was dramatically strong in all layers. The expression levels of d‐β‐aspartic acid to the cystic content and perimatrix were significantly higher in congenital middle ear cholesteatoma than in the acquired type. Conclusions This study showed the expression levels of d‐β‐aspartic acid in middle ear cholesteatoma to differ significantly between congenital and acquired middle ear cholesteatoma. Our results indicate that overexpression of d‐β‐aspartic acid is likely to be involved in the pathogenesis of cholesteatoma, and we speculate that d‐β‐aspartic acid could be a novel biomarker for, and a therapeutic target in, congenital and acquired middle ear cholesteatoma. Level of Evidence 4 |
| first_indexed | 2024-04-14T03:23:55Z |
| format | Article |
| id | doaj.art-99793fe68d064d34a5df050e648858d0 |
| institution | Directory Open Access Journal |
| issn | 2378-8038 |
| language | English |
| last_indexed | 2024-04-14T03:23:55Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Laryngoscope Investigative Otolaryngology |
| spelling | doaj.art-99793fe68d064d34a5df050e648858d02022-12-22T02:15:14ZengWileyLaryngoscope Investigative Otolaryngology2378-80382022-08-01741155116310.1002/lio2.856Immunohistochemical localization of d‐β‐aspartic acid in congenital and acquired middle ear cholesteatomaShiori Kitaya0Ryoukichi Ikeda1Jun Suzuki2Hidetoshi Oshima3Yuri Nomura4Yusuke Kusano5Nobuo Ohta6Tetsuaki Kawase7Kazue Ise8Kazuhiro Murakami9Yasuhiro Nakamura10Hironobu Sasano11Yukio Katori12Department of Otolaryngology, Head and Neck Surgery Tohoku University Hospital Sendai JapanDepartment of Otolaryngology, Head and Neck Surgery Tohoku University Hospital Sendai JapanDepartment of Otolaryngology, Head and Neck Surgery Tohoku University Hospital Sendai JapanCenter for Otologic Surgery Sen‐en Rifu Hospital Rifu Miyagi JapanCenter for Otologic Surgery Sen‐en Rifu Hospital Rifu Miyagi JapanDepartment of Otolaryngology, Head and Neck Surgery Tohoku University Hospital Sendai JapanDivision of Otolaryngology Tohoku Medical and Pharmaceutical University Hospital Sendai JapanDepartment of Otolaryngology, Head and Neck Surgery Tohoku University Hospital Sendai JapanTechnical Services Division Tohoku Medical and Pharmaceutical University Sendai JapanDivision of Pathology Tohoku Medical and Pharmaceutical University Hospital Sendai JapanDivision of Pathology Tohoku Medical and Pharmaceutical University Hospital Sendai JapanDepartment of Pathology Tohoku University School of Medicine Sendai JapanDepartment of Otolaryngology, Head and Neck Surgery Tohoku University Hospital Sendai JapanAbstract Objective/Hypothesis Middle ear cholesteatoma is characterized by abnormal growth of the keratinizing squamous epithelium of the temporal bone. d‐β‐aspartic acid is the major isomer of d‐aspartic acid found in elderly tissue. We assessed the immunoreactivity to k‐β‐aspartic acid of congenital and acquired middle ear cholesteatomas. Study Design Case–control studies. Material and Methods Tissue samples were collected from 21 patients comprising 21 ears with congenital middle ear cholesteatoma and 26 patients comprising 29 ears with acquired type. Their clinical and histopathological features were investigated. We divided the middle ear cholesteatoma samples into three layers: the perimatrix, matrix, and cystic contents. The patterns of immunoreactivity to d‐β‐aspartic acid expression were then assessed immunohistochemically. Results Two patterns of immunoreactivity to d‐β‐aspartic acid were detected in middle ear cholesteatoma: infiltrative and diffuse. In congenital middle ear cholesteatoma, d‐β‐aspartic acid expression was observed throughout all the layers (perimatrix, matrix, and cystic contents), and immunoreactivity to d‐β‐aspartic acid was dramatically strong in all layers. The expression levels of d‐β‐aspartic acid to the cystic content and perimatrix were significantly higher in congenital middle ear cholesteatoma than in the acquired type. Conclusions This study showed the expression levels of d‐β‐aspartic acid in middle ear cholesteatoma to differ significantly between congenital and acquired middle ear cholesteatoma. Our results indicate that overexpression of d‐β‐aspartic acid is likely to be involved in the pathogenesis of cholesteatoma, and we speculate that d‐β‐aspartic acid could be a novel biomarker for, and a therapeutic target in, congenital and acquired middle ear cholesteatoma. Level of Evidence 4https://doi.org/10.1002/lio2.856acquired middle ear cholesteatomacongenital middle ear cholesteatomad‐β‐aspartic acidmatrixperimatrix |
| spellingShingle | Shiori Kitaya Ryoukichi Ikeda Jun Suzuki Hidetoshi Oshima Yuri Nomura Yusuke Kusano Nobuo Ohta Tetsuaki Kawase Kazue Ise Kazuhiro Murakami Yasuhiro Nakamura Hironobu Sasano Yukio Katori Immunohistochemical localization of d‐β‐aspartic acid in congenital and acquired middle ear cholesteatoma Laryngoscope Investigative Otolaryngology acquired middle ear cholesteatoma congenital middle ear cholesteatoma d‐β‐aspartic acid matrix perimatrix |
| title | Immunohistochemical localization of d‐β‐aspartic acid in congenital and acquired middle ear cholesteatoma |
| title_full | Immunohistochemical localization of d‐β‐aspartic acid in congenital and acquired middle ear cholesteatoma |
| title_fullStr | Immunohistochemical localization of d‐β‐aspartic acid in congenital and acquired middle ear cholesteatoma |
| title_full_unstemmed | Immunohistochemical localization of d‐β‐aspartic acid in congenital and acquired middle ear cholesteatoma |
| title_short | Immunohistochemical localization of d‐β‐aspartic acid in congenital and acquired middle ear cholesteatoma |
| title_sort | immunohistochemical localization of d β aspartic acid in congenital and acquired middle ear cholesteatoma |
| topic | acquired middle ear cholesteatoma congenital middle ear cholesteatoma d‐β‐aspartic acid matrix perimatrix |
| url | https://doi.org/10.1002/lio2.856 |
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