Design of Functional Pluronic-Based Precursors for Tailoring Hydrogel Thermoresponsiveness and Cell-Adhesive Properties
In this study, functional Pluronic F127 precursors were designed and synthesized for the preparation of thermosensitive hydrogels. Using linear Pluronic thioacetate and Pluronic multi-acrylate precursors, F127-based hydrogels were prepared through thioacetate deprotection-mediated Michael-type addit...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/16/7/2749 |
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| author | Giulia Camana Mirko Tavano Min Li Franca Castiglione Filippo Rossi Francesco Cellesi |
| author_facet | Giulia Camana Mirko Tavano Min Li Franca Castiglione Filippo Rossi Francesco Cellesi |
| author_sort | Giulia Camana |
| collection | DOAJ |
| description | In this study, functional Pluronic F127 precursors were designed and synthesized for the preparation of thermosensitive hydrogels. Using linear Pluronic thioacetate and Pluronic multi-acrylate precursors, F127-based hydrogels were prepared through thioacetate deprotection-mediated Michael-type addition. The properties of these gels were compared to those obtained through free radical crosslinking of F127 diacrylate. Temperature was found to have a clear influence on gel swelling as a result of F127 thermoresponsiveness. The macromolecular architecture and functionality of the precursors were also optimized and characterized in terms of gelation kinetics and drug diffusion. In vitro tests were conducted on fibroblasts and endothelial cells to assess their response to cellular adhesion with Pluronic gels that were functionalized with an RGD peptide or pretreated with serum proteins to promote cell adhesion. This study provides a method for creating tailored hydrogels suitable for various biomedical applications, such as soft-tissue engineering, cell encapsulation, wound healing, and sustained delivery of therapeutic molecules. |
| first_indexed | 2024-03-11T05:32:00Z |
| format | Article |
| id | doaj.art-997b46fbc93b4ddbac143e2ea2d75355 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-11T05:32:00Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
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| series | Materials |
| spelling | doaj.art-997b46fbc93b4ddbac143e2ea2d753552023-11-17T17:04:33ZengMDPI AGMaterials1996-19442023-03-01167274910.3390/ma16072749Design of Functional Pluronic-Based Precursors for Tailoring Hydrogel Thermoresponsiveness and Cell-Adhesive PropertiesGiulia Camana0Mirko Tavano1Min Li2Franca Castiglione3Filippo Rossi4Francesco Cellesi5Dipartimento di Chimica, Materiali ed Ingegneria Chimica “G. Natta”, Politecnico di Milano, Via Mancinelli 7, 20131 Milan, ItalyDipartimento di Chimica, Materiali ed Ingegneria Chimica “G. Natta”, Politecnico di Milano, Via Mancinelli 7, 20131 Milan, ItalyRenal Research Laboratory, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Via Pace 9, 20122 Milan, ItalyDipartimento di Chimica, Materiali ed Ingegneria Chimica “G. Natta”, Politecnico di Milano, Via Mancinelli 7, 20131 Milan, ItalyDipartimento di Chimica, Materiali ed Ingegneria Chimica “G. Natta”, Politecnico di Milano, Via Mancinelli 7, 20131 Milan, ItalyDipartimento di Chimica, Materiali ed Ingegneria Chimica “G. Natta”, Politecnico di Milano, Via Mancinelli 7, 20131 Milan, ItalyIn this study, functional Pluronic F127 precursors were designed and synthesized for the preparation of thermosensitive hydrogels. Using linear Pluronic thioacetate and Pluronic multi-acrylate precursors, F127-based hydrogels were prepared through thioacetate deprotection-mediated Michael-type addition. The properties of these gels were compared to those obtained through free radical crosslinking of F127 diacrylate. Temperature was found to have a clear influence on gel swelling as a result of F127 thermoresponsiveness. The macromolecular architecture and functionality of the precursors were also optimized and characterized in terms of gelation kinetics and drug diffusion. In vitro tests were conducted on fibroblasts and endothelial cells to assess their response to cellular adhesion with Pluronic gels that were functionalized with an RGD peptide or pretreated with serum proteins to promote cell adhesion. This study provides a method for creating tailored hydrogels suitable for various biomedical applications, such as soft-tissue engineering, cell encapsulation, wound healing, and sustained delivery of therapeutic molecules.https://www.mdpi.com/1996-1944/16/7/2749PluronicF127gelMichael-type additionthermoresponsivecell adhesion |
| spellingShingle | Giulia Camana Mirko Tavano Min Li Franca Castiglione Filippo Rossi Francesco Cellesi Design of Functional Pluronic-Based Precursors for Tailoring Hydrogel Thermoresponsiveness and Cell-Adhesive Properties Materials Pluronic F127 gel Michael-type addition thermoresponsive cell adhesion |
| title | Design of Functional Pluronic-Based Precursors for Tailoring Hydrogel Thermoresponsiveness and Cell-Adhesive Properties |
| title_full | Design of Functional Pluronic-Based Precursors for Tailoring Hydrogel Thermoresponsiveness and Cell-Adhesive Properties |
| title_fullStr | Design of Functional Pluronic-Based Precursors for Tailoring Hydrogel Thermoresponsiveness and Cell-Adhesive Properties |
| title_full_unstemmed | Design of Functional Pluronic-Based Precursors for Tailoring Hydrogel Thermoresponsiveness and Cell-Adhesive Properties |
| title_short | Design of Functional Pluronic-Based Precursors for Tailoring Hydrogel Thermoresponsiveness and Cell-Adhesive Properties |
| title_sort | design of functional pluronic based precursors for tailoring hydrogel thermoresponsiveness and cell adhesive properties |
| topic | Pluronic F127 gel Michael-type addition thermoresponsive cell adhesion |
| url | https://www.mdpi.com/1996-1944/16/7/2749 |
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