Survival and Genome Evolution Signatures of <i>Klebsiella pneumoniae</i> Isolates Originated in Seven Species of Aquatic Animals
<i>Klebsiella pneumoniae</i> can cause life-threatening pneumonia in humans. The bacterium is also the causative agent of nosocomial infection diseases. In our recent research, we reported, for the first time, the presence of <i>K. pneumoniae</i> in fourteen species of aquati...
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MDPI AG
2023-04-01
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| Online Access: | https://www.mdpi.com/1424-2818/15/4/527 |
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| author | Huiqiong Guan Lu Xie Lanming Chen |
| author_facet | Huiqiong Guan Lu Xie Lanming Chen |
| author_sort | Huiqiong Guan |
| collection | DOAJ |
| description | <i>Klebsiella pneumoniae</i> can cause life-threatening pneumonia in humans. The bacterium is also the causative agent of nosocomial infection diseases. In our recent research, we reported, for the first time, the presence of <i>K. pneumoniae</i> in fourteen species of aquatic animals sampled in Shanghai, China. Here, we further investigated the bacterial survival and genome evolution traits. The results revealed that <i>K. pneumoniae</i> isolates (<i>n</i> = 7), recovered from 7 species of commonly consumed aquatic animals, had multiple antibiotic and heavy metal resistance profiles. The isolates were capable of growing vigorously at pH 4.5−7.5 and 0.5−1.0% NaCl in TSB medium at 37 °C. Draft genome sequences of the <i>K. pneumoniae</i> isolates were determined (5,256,522−5,857,823 bp, 56.35–57.81% GC contents), which carried many mobile genetic elements, including genomic islands (<i>n</i> = 87), prophages (<i>n</i> = 14), integrons (<i>n</i> = 4), and insertion sequences (<i>n</i> = 22), indicating possible active horizontal gene transfer during the genome evolution. Meanwhile, numerous strain-specific (<i>n</i> = 199−605) genes, antibiotic resistance (<i>n</i> = 20−35, e.g., β-lactamase) genes, and virulence (<i>n</i> = 43−59, e.g., enterobactin)-related genes, were also identified, demonstrating considerable genome variation in the <i>K. pneumoniae</i> isolates. Overall, the results of this study fill prior gaps in understanding the <i>K. pneumoniae</i> genomes derived from aquatic animals. |
| first_indexed | 2024-03-11T05:06:15Z |
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| spelling | doaj.art-9981224cdadf4b1b825ab74a3b27483c2023-11-17T18:56:53ZengMDPI AGDiversity1424-28182023-04-0115452710.3390/d15040527Survival and Genome Evolution Signatures of <i>Klebsiella pneumoniae</i> Isolates Originated in Seven Species of Aquatic AnimalsHuiqiong Guan0Lu Xie1Lanming Chen2Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Shanghai 201306, ChinaShanghai-MOST Key Laboratory of Health and Disease Genomics, Institute for Genome and Bioinformatics, Shanghai Institute for Biomedical and Pharmaceutical Technologies, Shanghai 200032, ChinaKey Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Shanghai 201306, China<i>Klebsiella pneumoniae</i> can cause life-threatening pneumonia in humans. The bacterium is also the causative agent of nosocomial infection diseases. In our recent research, we reported, for the first time, the presence of <i>K. pneumoniae</i> in fourteen species of aquatic animals sampled in Shanghai, China. Here, we further investigated the bacterial survival and genome evolution traits. The results revealed that <i>K. pneumoniae</i> isolates (<i>n</i> = 7), recovered from 7 species of commonly consumed aquatic animals, had multiple antibiotic and heavy metal resistance profiles. The isolates were capable of growing vigorously at pH 4.5−7.5 and 0.5−1.0% NaCl in TSB medium at 37 °C. Draft genome sequences of the <i>K. pneumoniae</i> isolates were determined (5,256,522−5,857,823 bp, 56.35–57.81% GC contents), which carried many mobile genetic elements, including genomic islands (<i>n</i> = 87), prophages (<i>n</i> = 14), integrons (<i>n</i> = 4), and insertion sequences (<i>n</i> = 22), indicating possible active horizontal gene transfer during the genome evolution. Meanwhile, numerous strain-specific (<i>n</i> = 199−605) genes, antibiotic resistance (<i>n</i> = 20−35, e.g., β-lactamase) genes, and virulence (<i>n</i> = 43−59, e.g., enterobactin)-related genes, were also identified, demonstrating considerable genome variation in the <i>K. pneumoniae</i> isolates. Overall, the results of this study fill prior gaps in understanding the <i>K. pneumoniae</i> genomes derived from aquatic animals.https://www.mdpi.com/1424-2818/15/4/527<i>Klebsiella pneumoniae</i>aquatic animalgenomemobile genetic elementvirulenceantibiotic resistance |
| spellingShingle | Huiqiong Guan Lu Xie Lanming Chen Survival and Genome Evolution Signatures of <i>Klebsiella pneumoniae</i> Isolates Originated in Seven Species of Aquatic Animals Diversity <i>Klebsiella pneumoniae</i> aquatic animal genome mobile genetic element virulence antibiotic resistance |
| title | Survival and Genome Evolution Signatures of <i>Klebsiella pneumoniae</i> Isolates Originated in Seven Species of Aquatic Animals |
| title_full | Survival and Genome Evolution Signatures of <i>Klebsiella pneumoniae</i> Isolates Originated in Seven Species of Aquatic Animals |
| title_fullStr | Survival and Genome Evolution Signatures of <i>Klebsiella pneumoniae</i> Isolates Originated in Seven Species of Aquatic Animals |
| title_full_unstemmed | Survival and Genome Evolution Signatures of <i>Klebsiella pneumoniae</i> Isolates Originated in Seven Species of Aquatic Animals |
| title_short | Survival and Genome Evolution Signatures of <i>Klebsiella pneumoniae</i> Isolates Originated in Seven Species of Aquatic Animals |
| title_sort | survival and genome evolution signatures of i klebsiella pneumoniae i isolates originated in seven species of aquatic animals |
| topic | <i>Klebsiella pneumoniae</i> aquatic animal genome mobile genetic element virulence antibiotic resistance |
| url | https://www.mdpi.com/1424-2818/15/4/527 |
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