In vivo anti-ulcerogenic effect of okra (Abelmoschus esculentus) on ethanol-induced acute gastric mucosal lesions
Context: Okra, Abelmoschus esculentus (L.) (Malvaceae), is a medicinal plant widely used in Turkish traditional medicine for the treatment of various diseases such as ulcers and gastritis. Objective: In the present study, we evaluated the gastroprotective effect of okra against ethanol-induced acute...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Pharmaceutical Biology |
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| Online Access: | http://dx.doi.org/10.1080/13880209.2018.1442481 |
| _version_ | 1811279430803259392 |
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| author | Deniz Ortaç Mustafa Cemek Turan Karaca Mehmet E. Büyükokuroğlu Zafer Ö. Özdemir Ayşe Tuba Kocaman Sadık Göneş |
| author_facet | Deniz Ortaç Mustafa Cemek Turan Karaca Mehmet E. Büyükokuroğlu Zafer Ö. Özdemir Ayşe Tuba Kocaman Sadık Göneş |
| author_sort | Deniz Ortaç |
| collection | DOAJ |
| description | Context: Okra, Abelmoschus esculentus (L.) (Malvaceae), is a medicinal plant widely used in Turkish traditional medicine for the treatment of various diseases such as ulcers and gastritis. Objective: In the present study, we evaluated the gastroprotective effect of okra against ethanol-induced acute gastric mucosal injury in animal models. Materials and methods: Wistar rats were treated with 500, 250 or 100 mg/kg okra; 20 mg/kg famotidine (Fam); and 75 mg/kg quercetin (Que). Following a 60 min period, all the rats were given 1 mL of ethanol (80%). One hour after the administration of ethanol, all groups were sacrificed. Results: At 5000 mg/kg, the extract produced (okra) no signs of toxicity in animals. Okra 500, 250, 100, Fam 20 and Que 75 inhibited ulcer formation by 81.0, 67.5, 67.0, 76.3 and 72.4%, respectively. Okra 500 significantly decreased edema, hemorrhage and inflammation scores compared with the ethanol group (p < 0.05). The oxidant levels decreased significantly in the all study groups compared within ethanol group (p < 0.001). Serum β-carotene and retinol levels significantly increased 40.2 and 45.4% in the okra 500 group. In okra 500, 250 and Fam 20 groups, apoptosis significantly decreased (p < 0.001), while okra 500, 250 and Fam 20 groups showed a higher percentage of cell proliferation compared with the ethanol group (p < 0.001). Discussion and conclusions: Our in vivo data indicate that okra has a gastroprotective effect against ethanol and could reduce the gastric ulcer as seen from biochemical and histopathological results. We suggest that okra could be a possible therapeutic antiulcer agent. |
| first_indexed | 2024-04-13T00:54:31Z |
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| id | doaj.art-9987e4a17bba499aa2f274e52ac0c17f |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-13T00:54:31Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-9987e4a17bba499aa2f274e52ac0c17f2022-12-22T03:09:44ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162018-01-0156116517510.1080/13880209.2018.14424811442481In vivo anti-ulcerogenic effect of okra (Abelmoschus esculentus) on ethanol-induced acute gastric mucosal lesionsDeniz Ortaç0Mustafa Cemek1Turan Karaca2Mehmet E. Büyükokuroğlu3Zafer Ö. Özdemir4Ayşe Tuba Kocaman5Sadık Göneş6Yıldız Technical UniversityYıldız Technical UniversityUniversity of TrakyaSakarya UniversityKırklareli UniversityYıldız Technical UniversityYıldız Technical UniversityContext: Okra, Abelmoschus esculentus (L.) (Malvaceae), is a medicinal plant widely used in Turkish traditional medicine for the treatment of various diseases such as ulcers and gastritis. Objective: In the present study, we evaluated the gastroprotective effect of okra against ethanol-induced acute gastric mucosal injury in animal models. Materials and methods: Wistar rats were treated with 500, 250 or 100 mg/kg okra; 20 mg/kg famotidine (Fam); and 75 mg/kg quercetin (Que). Following a 60 min period, all the rats were given 1 mL of ethanol (80%). One hour after the administration of ethanol, all groups were sacrificed. Results: At 5000 mg/kg, the extract produced (okra) no signs of toxicity in animals. Okra 500, 250, 100, Fam 20 and Que 75 inhibited ulcer formation by 81.0, 67.5, 67.0, 76.3 and 72.4%, respectively. Okra 500 significantly decreased edema, hemorrhage and inflammation scores compared with the ethanol group (p < 0.05). The oxidant levels decreased significantly in the all study groups compared within ethanol group (p < 0.001). Serum β-carotene and retinol levels significantly increased 40.2 and 45.4% in the okra 500 group. In okra 500, 250 and Fam 20 groups, apoptosis significantly decreased (p < 0.001), while okra 500, 250 and Fam 20 groups showed a higher percentage of cell proliferation compared with the ethanol group (p < 0.001). Discussion and conclusions: Our in vivo data indicate that okra has a gastroprotective effect against ethanol and could reduce the gastric ulcer as seen from biochemical and histopathological results. We suggest that okra could be a possible therapeutic antiulcer agent.http://dx.doi.org/10.1080/13880209.2018.1442481gastroprotectionapoptosisgastric ulcerimmunohistochemistryulcer indexulcer inhibition |
| spellingShingle | Deniz Ortaç Mustafa Cemek Turan Karaca Mehmet E. Büyükokuroğlu Zafer Ö. Özdemir Ayşe Tuba Kocaman Sadık Göneş In vivo anti-ulcerogenic effect of okra (Abelmoschus esculentus) on ethanol-induced acute gastric mucosal lesions Pharmaceutical Biology gastroprotection apoptosis gastric ulcer immunohistochemistry ulcer index ulcer inhibition |
| title | In vivo anti-ulcerogenic effect of okra (Abelmoschus esculentus) on ethanol-induced acute gastric mucosal lesions |
| title_full | In vivo anti-ulcerogenic effect of okra (Abelmoschus esculentus) on ethanol-induced acute gastric mucosal lesions |
| title_fullStr | In vivo anti-ulcerogenic effect of okra (Abelmoschus esculentus) on ethanol-induced acute gastric mucosal lesions |
| title_full_unstemmed | In vivo anti-ulcerogenic effect of okra (Abelmoschus esculentus) on ethanol-induced acute gastric mucosal lesions |
| title_short | In vivo anti-ulcerogenic effect of okra (Abelmoschus esculentus) on ethanol-induced acute gastric mucosal lesions |
| title_sort | in vivo anti ulcerogenic effect of okra abelmoschus esculentus on ethanol induced acute gastric mucosal lesions |
| topic | gastroprotection apoptosis gastric ulcer immunohistochemistry ulcer index ulcer inhibition |
| url | http://dx.doi.org/10.1080/13880209.2018.1442481 |
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