Chitosan nanoparticle and its effect on Neoscytalidium novaehollandiae, the causal agent of mulberry canker in Tehran
Fungal pathogen “Neoscytalidium novaehollandiae” is the causal agent of trunk canker in mulberry trees. Mulberry is considered as most valuable tree for landscaping in Tehran. Here in, for the first time, chitosan nanoparticles (CSNPs) were used to inhibit canker disease causal agent of mulberry. Fo...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024046978 |
| _version_ | 1797235549156671488 |
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| author | Naemeh Mohammadi Naser Safaie Maryam Nikkhah Sajad Moradi |
| author_facet | Naemeh Mohammadi Naser Safaie Maryam Nikkhah Sajad Moradi |
| author_sort | Naemeh Mohammadi |
| collection | DOAJ |
| description | Fungal pathogen “Neoscytalidium novaehollandiae” is the causal agent of trunk canker in mulberry trees. Mulberry is considered as most valuable tree for landscaping in Tehran. Here in, for the first time, chitosan nanoparticles (CSNPs) were used to inhibit canker disease causal agent of mulberry. For this purpose, CSNPs were synthesized with a yield of 86%, and after characterization of the synthesized nanoparticles, the growth inhibition rate of fungus (GI%) was evaluated. The results of in vitro assays showed that the concentration of 1500 ppm significantly (P ≤ 0.05) decreased the radial growth of the fungus in comparison with control. For in vivo experiments, 2-year-old branches from healthy randomly selected mulberry trees in the landscape, were inoculated artificially in the laboratory with mycelial plugs from a 7-day-old culture of fungus. The infected branches were then treated with 500, 1000, and 1500 ppm of CSNPs. The results indicated that the disease severity (DS%) in all the treatments and the control plants increased over time. However, the slope of the changes in DS was less in CSNPs treated compared to control. This effect was concentration dependent so that no disease progress was observed at 1500 ppm of CSNPs. The findings indicate the effectiveness of CSNPs in control of canker disease of mulberry caused by N. novaehollandiae. |
| first_indexed | 2024-04-24T16:49:43Z |
| format | Article |
| id | doaj.art-9988a05f74524a1d89538d5c1c2eaf90 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T16:49:43Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-9988a05f74524a1d89538d5c1c2eaf902024-03-29T05:50:46ZengElsevierHeliyon2405-84402024-04-01107e28666Chitosan nanoparticle and its effect on Neoscytalidium novaehollandiae, the causal agent of mulberry canker in TehranNaemeh Mohammadi0Naser Safaie1Maryam Nikkhah2Sajad Moradi3Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, IranDepartment of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran; Corresponding author.Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, IranNano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, IranFungal pathogen “Neoscytalidium novaehollandiae” is the causal agent of trunk canker in mulberry trees. Mulberry is considered as most valuable tree for landscaping in Tehran. Here in, for the first time, chitosan nanoparticles (CSNPs) were used to inhibit canker disease causal agent of mulberry. For this purpose, CSNPs were synthesized with a yield of 86%, and after characterization of the synthesized nanoparticles, the growth inhibition rate of fungus (GI%) was evaluated. The results of in vitro assays showed that the concentration of 1500 ppm significantly (P ≤ 0.05) decreased the radial growth of the fungus in comparison with control. For in vivo experiments, 2-year-old branches from healthy randomly selected mulberry trees in the landscape, were inoculated artificially in the laboratory with mycelial plugs from a 7-day-old culture of fungus. The infected branches were then treated with 500, 1000, and 1500 ppm of CSNPs. The results indicated that the disease severity (DS%) in all the treatments and the control plants increased over time. However, the slope of the changes in DS was less in CSNPs treated compared to control. This effect was concentration dependent so that no disease progress was observed at 1500 ppm of CSNPs. The findings indicate the effectiveness of CSNPs in control of canker disease of mulberry caused by N. novaehollandiae.http://www.sciencedirect.com/science/article/pii/S2405844024046978BiopolymerLandscape treesNano fungicideNanomaterial |
| spellingShingle | Naemeh Mohammadi Naser Safaie Maryam Nikkhah Sajad Moradi Chitosan nanoparticle and its effect on Neoscytalidium novaehollandiae, the causal agent of mulberry canker in Tehran Heliyon Biopolymer Landscape trees Nano fungicide Nanomaterial |
| title | Chitosan nanoparticle and its effect on Neoscytalidium novaehollandiae, the causal agent of mulberry canker in Tehran |
| title_full | Chitosan nanoparticle and its effect on Neoscytalidium novaehollandiae, the causal agent of mulberry canker in Tehran |
| title_fullStr | Chitosan nanoparticle and its effect on Neoscytalidium novaehollandiae, the causal agent of mulberry canker in Tehran |
| title_full_unstemmed | Chitosan nanoparticle and its effect on Neoscytalidium novaehollandiae, the causal agent of mulberry canker in Tehran |
| title_short | Chitosan nanoparticle and its effect on Neoscytalidium novaehollandiae, the causal agent of mulberry canker in Tehran |
| title_sort | chitosan nanoparticle and its effect on neoscytalidium novaehollandiae the causal agent of mulberry canker in tehran |
| topic | Biopolymer Landscape trees Nano fungicide Nanomaterial |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024046978 |
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