Pathological and molecular findings of avian avulavirus type 1 outbreak in pigeons (Columba livia) of southern Brazil
ABSTRACT: The Newcastle disease, caused by avian avulavirus type 1 strains (APMV-1) is an important avian disease involved into high rates of mortality and economic losses. Several outbreaks have been reported over the last 30 years in Columbiformes in different parts of the world, caused by a adapt...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Colégio Brasileiro de Patologia Animal (CBPA)
|
| Series: | Pesquisa Veterinária Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018001202254&lng=en&tlng=en |
| _version_ | 1827191451332640768 |
|---|---|
| author | Suyene O. Souza Gabriela Fredo Priscilla M. Dupont Ronaldo V. Leite-Filho Jens P. Teifke Saulo P. Pavarini Cláudio W. Canal David Driemeier |
| author_facet | Suyene O. Souza Gabriela Fredo Priscilla M. Dupont Ronaldo V. Leite-Filho Jens P. Teifke Saulo P. Pavarini Cláudio W. Canal David Driemeier |
| author_sort | Suyene O. Souza |
| collection | DOAJ |
| description | ABSTRACT: The Newcastle disease, caused by avian avulavirus type 1 strains (APMV-1) is an important avian disease involved into high rates of mortality and economic losses. Several outbreaks have been reported over the last 30 years in Columbiformes in different parts of the world, caused by a adapted variant strain of AAvV-1, called pigeon paramyxovirus type 1 (PPMV-1). A high mortality associated with an outbreak was analyzed in free-living pigeons (Columba livia) in a public square in Porto Alegre in Southern Brazil. A total of 24 pigeons moribund or freshly dead, within five weeks interval were submitted to necropsy, histopathological, immunohistochemical (anti-Newcastle), and RT-PCR followed by sequencing of the amplification products analysis. They presented neurological signs, non-suppurative encephalitis and encephalomyelitis, and mononuclear inflammatory infiltrate in different organs. Immunohistochemical analysis in nine pigeons tissue showed that anti-Newcastle was expressed in brain, kidney, liver and pancreas. The RT-PCR test for the M protein of Newcastle disease virus was positive in six pigeons. The differential diagnosis of Influenza, West Nile, Mycoplasma gallisepticum and Mycoplasma synoviae in all pigeons presented negative results. The sequence of amino acids in the cleavage site region of the F protein was 112RRQKRF117 classifying the strain as virulent. The phylogenetic analysis classified this virus strain into Class II and VI genotype. |
| first_indexed | 2025-03-21T08:25:58Z |
| format | Article |
| id | doaj.art-9988e2eb6af6450c9d65c69dac6a3b82 |
| institution | Directory Open Access Journal |
| issn | 1678-5150 |
| language | English |
| last_indexed | 2025-03-21T08:25:58Z |
| publisher | Colégio Brasileiro de Patologia Animal (CBPA) |
| record_format | Article |
| series | Pesquisa Veterinária Brasileira |
| spelling | doaj.art-9988e2eb6af6450c9d65c69dac6a3b822024-07-12T12:10:26ZengColégio Brasileiro de Patologia Animal (CBPA)Pesquisa Veterinária Brasileira1678-515038122254226110.1590/1678-5150-pvb-5528S0100-736X2018001202254Pathological and molecular findings of avian avulavirus type 1 outbreak in pigeons (Columba livia) of southern BrazilSuyene O. SouzaGabriela FredoPriscilla M. DupontRonaldo V. Leite-FilhoJens P. TeifkeSaulo P. PavariniCláudio W. CanalDavid DriemeierABSTRACT: The Newcastle disease, caused by avian avulavirus type 1 strains (APMV-1) is an important avian disease involved into high rates of mortality and economic losses. Several outbreaks have been reported over the last 30 years in Columbiformes in different parts of the world, caused by a adapted variant strain of AAvV-1, called pigeon paramyxovirus type 1 (PPMV-1). A high mortality associated with an outbreak was analyzed in free-living pigeons (Columba livia) in a public square in Porto Alegre in Southern Brazil. A total of 24 pigeons moribund or freshly dead, within five weeks interval were submitted to necropsy, histopathological, immunohistochemical (anti-Newcastle), and RT-PCR followed by sequencing of the amplification products analysis. They presented neurological signs, non-suppurative encephalitis and encephalomyelitis, and mononuclear inflammatory infiltrate in different organs. Immunohistochemical analysis in nine pigeons tissue showed that anti-Newcastle was expressed in brain, kidney, liver and pancreas. The RT-PCR test for the M protein of Newcastle disease virus was positive in six pigeons. The differential diagnosis of Influenza, West Nile, Mycoplasma gallisepticum and Mycoplasma synoviae in all pigeons presented negative results. The sequence of amino acids in the cleavage site region of the F protein was 112RRQKRF117 classifying the strain as virulent. The phylogenetic analysis classified this virus strain into Class II and VI genotype.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018001202254&lng=en&tlng=enAvulavirus aviário tipo 1Columba liviapombosPPMV-1Doença de NewcastleencefalomieliteColumbiformespatologia |
| spellingShingle | Suyene O. Souza Gabriela Fredo Priscilla M. Dupont Ronaldo V. Leite-Filho Jens P. Teifke Saulo P. Pavarini Cláudio W. Canal David Driemeier Pathological and molecular findings of avian avulavirus type 1 outbreak in pigeons (Columba livia) of southern Brazil Pesquisa Veterinária Brasileira Avulavirus aviário tipo 1 Columba livia pombos PPMV-1 Doença de Newcastle encefalomielite Columbiformes patologia |
| title | Pathological and molecular findings of avian avulavirus type 1 outbreak in pigeons (Columba livia) of southern Brazil |
| title_full | Pathological and molecular findings of avian avulavirus type 1 outbreak in pigeons (Columba livia) of southern Brazil |
| title_fullStr | Pathological and molecular findings of avian avulavirus type 1 outbreak in pigeons (Columba livia) of southern Brazil |
| title_full_unstemmed | Pathological and molecular findings of avian avulavirus type 1 outbreak in pigeons (Columba livia) of southern Brazil |
| title_short | Pathological and molecular findings of avian avulavirus type 1 outbreak in pigeons (Columba livia) of southern Brazil |
| title_sort | pathological and molecular findings of avian avulavirus type 1 outbreak in pigeons columba livia of southern brazil |
| topic | Avulavirus aviário tipo 1 Columba livia pombos PPMV-1 Doença de Newcastle encefalomielite Columbiformes patologia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018001202254&lng=en&tlng=en |
| work_keys_str_mv | AT suyeneosouza pathologicalandmolecularfindingsofavianavulavirustype1outbreakinpigeonscolumbaliviaofsouthernbrazil AT gabrielafredo pathologicalandmolecularfindingsofavianavulavirustype1outbreakinpigeonscolumbaliviaofsouthernbrazil AT priscillamdupont pathologicalandmolecularfindingsofavianavulavirustype1outbreakinpigeonscolumbaliviaofsouthernbrazil AT ronaldovleitefilho pathologicalandmolecularfindingsofavianavulavirustype1outbreakinpigeonscolumbaliviaofsouthernbrazil AT jenspteifke pathologicalandmolecularfindingsofavianavulavirustype1outbreakinpigeonscolumbaliviaofsouthernbrazil AT sauloppavarini pathologicalandmolecularfindingsofavianavulavirustype1outbreakinpigeonscolumbaliviaofsouthernbrazil AT claudiowcanal pathologicalandmolecularfindingsofavianavulavirustype1outbreakinpigeonscolumbaliviaofsouthernbrazil AT daviddriemeier pathologicalandmolecularfindingsofavianavulavirustype1outbreakinpigeonscolumbaliviaofsouthernbrazil |